Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002415
UniProt IDP51149
Primary gene name(s)RAB7A
Synonym gene name(s)RAB7
Protein nameRas-related protein Rab-7a
Protein functionKey regulator in endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Plays a central role, not only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient-transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes, or vacuoles and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses. Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function, e.g. Salmonella and sometimes by excluding RAB7A function, e.g. Mycobacterium. In concert with RAC1, plays a role in regulating the formation of RBs, ruffled borders in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA, PubMed:11179213, PubMed:12944476, PubMed:14617358, PubMed:20028791, PubMed:21255211. Regulates the endocytic trafficking of the EGF-EGFR complex by regulating its lysosomal degradation. Involved in the ADRB2-stimulated lipolysis through lipophagy, a cytosolic lipase-independent autophagic pathway, By similarity. Required for the exosomal release of SDCBP, CD63 and syndecan, PubMed:22660413. {ECO:0000250|UniProtKB:P51150, ECO:0000269|PubMed:11179213, ECO:0000269|PubMed:12944476, ECO:0000269|PubMed:14617358, ECO:0000269|PubMed:20028791, ECO:0000269|PubMed:22660413}.
Subcellular locationCytoplasmic vesicle, phagosome membrane {ECO:0000269|PubMed:12944476, ECO:0000269|PubMed:21255211};
Peripheral membrane protein {ECO:0000305};
Cytoplasmic side {ECO:0000305}. Late endosome membrane {ECO:0000269|PubMed:12944476, ECO:0000269|PubMed:14617358, ECO:0000269|PubMed:16176980, ECO:0000269|PubMed:20028791};
Peripheral membrane protein {ECO:0000305};
Cytoplasmic side {ECO:0000305}. Lysosome membrane {ECO:0000269|PubMed:12944476, ECO:0000269|PubMed:20028791};
Peripheral membrane protein {ECO:0000305};
Cytoplasmic side {ECO:0000305}. Melanosome membrane {ECO:0000269|PubMed:12643545, ECO:0000269|PubMed:17081065};
Peripheral membrane protein {ECO:0000305};
Cytoplasmic side {ECO:0000305}. Cytoplasmic vesicle, autophagosome membrane {ECO:0000269|PubMed:20028791};
Peripheral membrane protein {ECO:0000305};
Cytoplasmic side {ECO:0000305}. Lipid droplet {ECO:0000250|UniProtKB:P51150}. Endosome membrane {ECO:0000269|PubMed:22431521}. Note=Colocalizes with OSBPL1A at the late endosome, PubMed:16176980. Found in the ruffled border, a late endosomal-like compartment in the plasma membrane of bone-resorbing osteoclasts. Recruited to phagosomes containing S.aureus or Mycobacterium, PubMed:21255211. Lipid droplet localization is increased upon ADRB2 stimulation, By similarity. {ECO:0000250|UniProtKB:P51150, ECO:0000269|PubMed:16176980, ECO:0000269|PubMed:21255211}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P51149
Gene Ontology
(Biological Process)
Complete annatation
antigen processing and presentation of exogenous peptide antigen via MHC class II [GO:0019886];
bone resorption [GO:0045453];
early endosome to late endosome transport [GO:0045022];
endocytosis [GO:0006897];
endosome to lysosome transport [GO:0008333];
epidermal growth factor catabolic process [GO:0007174];
lipophagy [GO:0061724];
multi-organism intracellular transport [GO:1902583];
negative regulation of exosomal secretion [GO:1903542];
negative regulation of intralumenal vesicle formation [GO:1905366];
phagosome acidification [GO:0090383];
phagosome-lysosome fusion [GO:0090385];
phagosome maturation [GO:0090382];
positive regulation of exosomal secretion [GO:1903543];
positive regulation of protein catabolic process [GO:0045732];
positive regulation of viral process [GO:0048524];
protein targeting to lysosome [GO:0006622];
protein to membrane docking [GO:0022615];
protein transport [GO:0015031];
regulation of autophagosome assembly [GO:2000785];
retrograde transport, endosome to Golgi [GO:0042147];
small GTPase mediated signal transduction [GO:0007264];
viral release from host cell [GO:0019076]
Gene Ontology
(Molecular Function)
Complete annatation
GDP binding [GO:0019003];
GTPase activity [GO:0003924];
GTP binding [GO:0005525]
Gene Ontology
(Cellular Component)
Complete annatation
alveolar lamellar body [GO:0097208];
autophagosome membrane [GO:0000421];
cytosol [GO:0005829];
extracellular exosome [GO:0070062];
extrinsic component of lysosome membrane [GO:0032419];
Golgi apparatus [GO:0005794];
intracellular membrane-bounded organelle [GO:0043231];
late endosome [GO:0005770];
late endosome membrane [GO:0031902];
lipid particle [GO:0005811];
lysosomal membrane [GO:0005765];
lysosome [GO:0005764];
melanosome membrane [GO:0033162];
phagocytic vesicle [GO:0045335];
phagocytic vesicle membrane [GO:0030670];
pre-autophagosomal structure membrane [GO:0034045];
terminal bouton [GO:0043195];
vacuolar membrane [GO:0005774]
Protein-protein interaction113624
Phylogenetic treeP51149
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.358631791444070.2730917118268390.388214171519385
AZA vs. DISU0.09740211671458360.6996486528469030.969609310121983
AZA vs. IL70.03355131410659470.8610958445254910.999311006273513
AZA vs. SAHA-0.1133364296421170.6416202616986690.889277659511377
DISU vs. CD3-0.2740177476879760.4490752606899060.579618159058108
DISU vs. IL7-0.07325561505910780.770731462094590.94598742099013
DISU vs. SAHA-0.2085394726311250.4739075549183230.806461404494033
DMSO vs. AZA-0.02771061330294480.8681834503928821
DMSO vs. CD3-0.3974320559829880.2137457710992290.315384920823691
DMSO vs. DISU-0.1269449348330910.6021975542726130.939010728390408
DMSO vs. IL70.068496887266080.7024804631313920.938777844529559
DMSO vs. SAHA-0.09160307626846230.6970753223970820.908046523297791
HIV vs. Mock in Activation0.01583725586144370.9796633729082970.999983755607037
HIV vs. Mock in Latency-0.008963835226997390.9565877359698060.999834320637052
IL7 vs. CD3-0.3184115758088910.32110153618690.459068904006938
SAHA vs. CD3-0.4956417465401760.1610996206113620.254643516112149
SAHA vs. IL7-0.1494816245888350.5388912283393650.755425458599485
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.0953114 0.565017
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.014 0.961 0.983 0.988 0.834
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB04315 Guanosine-5&,39;-Diphosphate experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1T91 X-ray 1.9Å A/B/C/D=1-207.
1YHN X-ray 3.0Å A=1-207.
3LAW X-ray 2.8Å A/B/C/D/E=1-207.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
reverse transcriptase interacts with 24920821
Nef interacts with 19620308
capsid regulated by 24173214
Vif regulated by 24173214
Pr55(Gag) co-localizes with 19451649
Vpr upregulates 23874603
Pr55(Gag) regulated by 24173214
Nef requires 25496667
Envelope surface glycoprotein gp160; precursor regulated by 22072966

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04144 Endocytosis - Homo sapiens (human)
hsa04145 Phagosome - Homo sapiens (human)
hsa05132 Salmonella infection - Homo sapiens (human)
hsa05146 Amoebiasis - Homo sapiens (human)
hsa05152 Tuberculosis - Homo sapiens (human)