Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002394
UniProt IDP49023
Primary gene name(s)PXN
Synonym gene name(s)unknown
Protein namePaxillin
Protein functionCytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix, focal adhesion.
Subcellular locationCytoplasm, cytoskeleton {ECO:0000269|PubMed:20489202}. Cell junction, focal adhesion {ECO:0000269|PubMed:20489202, ECO:0000269|PubMed:23128389}. Cytoplasm, cell cortex {ECO:0000250|UniProtKB:Q8VI36}. Note=Colocalizes with integrins at the cell periphery. Colocalize with PXN to membrane ruffles and the leading edge of migrating cells, PubMed:23128389. {ECO:0000250, ECO:0000269|PubMed:23128389}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P49023
Gene Ontology
(Biological Process)
Complete annatation
cell adhesion [GO:0007155];
cell-matrix adhesion [GO:0007160];
cellular response to reactive oxygen species [GO:0034614];
epidermal growth factor receptor signaling pathway [GO:0007173];
growth hormone receptor signaling pathway [GO:0060396];
muscle contraction [GO:0006936];
signal complex assembly [GO:0007172];
signal transduction [GO:0007165];
transforming growth factor beta receptor signaling pathway [GO:0007179];
vascular endothelial growth factor receptor signaling pathway [GO:0048010]
Gene Ontology
(Molecular Function)
Complete annatation
beta-catenin binding [GO:0008013];
vinculin binding [GO:0017166];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
cell cortex [GO:0005938];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
focal adhesion [GO:0005925];
lamellipodium [GO:0030027];
microtubule associated complex [GO:0005875];
nucleoplasm [GO:0005654];
plasma membrane [GO:0005886];
stress fiber [GO:0001725]
Protein-protein interaction111787
Phylogenetic treeP49023
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-2.095278715778817.49121653598195e-101.10979166027249e-08
AZA vs. DISU-0.1759245721610960.4866306030440920.931429104131977
AZA vs. IL70.01959809188068030.9186907256949950.999311006273513
AZA vs. SAHA-0.6793634725634110.005519398169531290.0662943490099718
DISU vs. CD31.906731073442043.6110840029302e-073.87426905766333e-06
DISU vs. IL70.186182104311740.4615727365026610.802603102529058
DISU vs. SAHA-0.5008302567709490.08737772424380420.377198671009049
DMSO vs. AZA0.06869717390339550.6808308249429931
DMSO vs. CD32.150736199068351.07734821064298e-101.68189127392088e-09
DMSO vs. DISU0.2423103883765530.3212908574495710.822419732079662
DMSO vs. IL7-0.04147451957327120.8171390350026930.962956477091318
DMSO vs. SAHA-0.752882878710370.001466144044136470.0234318736894812
HIV vs. Mock in Activation0.1860427324768510.7650966075870730.999983755607037
HIV vs. Mock in Latency0.04022322860145510.8068870344404730.999834320637052
IL7 vs. CD32.120882823076092.34945618515781e-104.65965172090574e-09
SAHA vs. CD31.392781811760770.0001086760047330770.000518766991234549
SAHA vs. IL7-0.7015474750348860.004077761518217370.0332260416895182
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.145227 0.325277
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1KKY Model - A=142-157.
1KL0 Model - B=142-157.
1OW6 X-ray 2.3Å D/F=262-274.
1OW7 X-ray 2.6Å D/E/F=262-274.
1OW8 X-ray 2.8Å D/F=141-153.
2K2R NMR - B=3-12.
2O9V X-ray 1.6Å B=45-54.
2VZD X-ray 2.1Å C/D=1-20.
2VZG X-ray 1.8Å A=141-160.
2VZI X-ray 2.2Å A=262-315.
3GM1 X-ray 2.9Å C/D/E/F=262-274.
3PY7 X-ray 2.2Å A=1-10.
3RQE X-ray 2.8Å E=2-15.
3RQF X-ray 2.7Å E=141-153.
3RQG X-ray 2.5Å E=262-274.
3U3F X-ray 3.1Å E/F/G/H/I/J=261-277.
4EDN X-ray 2.9Å K/L/M/N/O/P/Q=1-20.
4R32 X-ray 3.7Å B/C=139-160.
4XGZ X-ray 2.5Å a/c/e/g/h/j/m/o/q/s/u/w=141-159.
4XH2 X-ray 2.0Å a/c/e/g/h/j=261-275.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Nef induces phosphorylation of 23317503
Tat recruits 22362758
matrix interacts with 22017400
Tat induces phosphorylation of 9621077
Envelope surface glycoprotein gp120 activates 23119100

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04062 Chemokine signaling pathway - Homo sapiens (human)
hsa04370 VEGF signaling pathway - Homo sapiens (human)
hsa04510 Focal adhesion - Homo sapiens (human)
hsa04670 Leukocyte transendothelial migration - Homo sapiens (human)
hsa04810 Regulation of actin cytoskeleton - Homo sapiens (human)
hsa05100 Bacterial invasion of epithelial cells - Homo sapiens (human)
hsa05203 Viral carcinogenesis - Homo sapiens (human)
hsa05205 Proteoglycans in cancer - Homo sapiens (human)