Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002391
UniProt IDQ9NQS3
Primary gene name(s)NECTIN3
Synonym gene name(s)PRR3, PVRL3
Protein nameNectin-3
Protein functionPlays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with NECTIN2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body. {ECO:0000269|PubMed:16216929}.
Subcellular locationCell membrane {ECO:0000305};
Single-pass membrane protein {ECO:0000305}. Cell junction, synapse, postsynaptic cell membrane {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9NQS3
Gene Ontology
(Biological Process)
Complete annatation
adherens junction organization [GO:0034332];
cell recognition [GO:0008037];
fertilization [GO:0009566];
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules [GO:0007157];
homophilic cell adhesion via plasma membrane adhesion molecules [GO:0007156];
lens morphogenesis in camera-type eye [GO:0002089];
protein localization to cell junction [GO:1902414];
retina morphogenesis in camera-type eye [GO:0060042];
single organismal cell-cell adhesion [GO:0016337];
spermatid development [GO:0007286]
Gene Ontology
(Molecular Function)
Complete annatation
Gene Ontology
(Cellular Component)
Complete annatation
Protein-protein interaction117441
Phylogenetic treeQ9NQS3
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.4871284670415090.1391048177485890.22868540718878
AZA vs. DISU0.2539709571051610.3195016040417120.870594587976108
AZA vs. IL7-0.4156083291628290.03335518163347980.566104966957544
AZA vs. SAHA0.6740359069613750.006306925919755790.0720777871981795
DISU vs. CD30.7289986344638860.04645502256423770.101826815491249
DISU vs. IL7-0.6782312948973270.007834395367684930.0997960526825774
DISU vs. SAHA0.4204216184020980.1548809520036320.506446263321052
DMSO vs. AZA-0.1542409200079040.3656557504322911
DMSO vs. CD30.3222340734985510.3157943524927010.42813552198547
DMSO vs. DISU-0.4098787545680590.09631877779390520.561988951844643
DMSO vs. IL7-0.2542633324047390.1642834112134780.67147816640446
DMSO vs. SAHA0.8205217003587190.000595083614759240.0123324077450763
HIV vs. Mock in Activation0.0735056135824470.9073788263831070.999983755607037
HIV vs. Mock in Latency0.1508252152617480.368857070653070.999834320637052
IL7 vs. CD30.08013496534320810.8038648482753140.871952709992358
SAHA vs. CD31.135206699783570.001569477646901190.00527515500956726
SAHA vs. IL71.084837435778031.25814102198696e-050.000368973108276876
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock 0.84 2.31E-06 5.39E-05
Infected vs. Bystander 0.845 2.13E-06 3.19E-05
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.0322247 0.903082
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
-0.023 0.01

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4FOM X-ray 3.9Å A=58-359.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat downregulates 23898208

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04514 Cell adhesion molecules (CAMs) - Homo sapiens (human)
hsa04520 Adherens junction - Homo sapiens (human)