Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002388
UniProt IDQ8TB72
Primary gene name(s)PUM2
Synonym gene name(s)KIAA0235, PUMH2
Protein namePumilio homolog 2
Protein functionSequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element, PRE, 5'-UGUANAUA-3', that is related to the Nanos Response Element, NRE, , PubMed:21397187. Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation, PubMed:22955276. Also mediates deadenylation-independent repression by promoting accessibility of miRNAs, PubMed:18776931, PubMed:22345517. Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3'-UTR and facilitating miRNA regulation, PubMed:22345517. Plays a role in cytoplasmic sensing of viral infection, PubMed:25340845. Represses a program of genes necessary to maintain genomic stability such as key mitotic, DNA repair and DNA replication factors. Its ability to repress those target mRNAs is regulated by the lncRNA NORAD, non-coding RNA activated by DNA damage which, due to its high abundance and multitude of PUMILIO binding sites, is able to sequester a significant fraction of PUM1 and PUM2 in the cytoplasm, PubMed:26724866. May regulate DCUN1D3 mRNA levels, PubMed:25349211. May support proliferation and self-renewal of stem cells. {ECO:0000269|PubMed:18776931, ECO:0000269|PubMed:21397187, ECO:0000269|PubMed:22345517, ECO:0000269|PubMed:22955276, ECO:0000269|PubMed:25340845, ECO:0000269|PubMed:25349211, ECO:0000269|PubMed:26724866}.
Subcellular locationCytoplasm {ECO:0000305|PubMed:19168546}. Cytoplasmic granule {ECO:0000269|PubMed:25340845}. Cytoplasm, perinuclear region {ECO:0000269|PubMed:19168546}. Note=The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules. Colocalizes with NANOS1 and SNAPIN in the perinuclear region of germ cells. {ECO:0000269|PubMed:25340845}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q8TB72
Gene Ontology
(Biological Process)
Complete annatation
positive regulation of gene silencing by miRNA [GO:2000637];
positive regulation of RIG-I signaling pathway [GO:1900246];
posttranscriptional regulation of gene expression [GO:0010608];
regulation of chromosome segregation [GO:0051983];
regulation of gene silencing by miRNA [GO:0060964];
regulation of mRNA stability [GO:0043488];
stress granule assembly [GO:0034063]
Gene Ontology
(Molecular Function)
Complete annatation
mRNA 3'-UTR binding [GO:0003730];
poly(A RNA binding [GO:0044822];
RNA binding [GO:0003723]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytoplasmic stress granule [GO:0010494];
nuclear membrane [GO:0031965];
perinuclear region of cytoplasm [GO:0048471]
Protein-protein interaction116949
Phylogenetic treeQ8TB72
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.1148968642851390.7251522306471410.80606935764538
AZA vs. DISU0.1105842891498750.6625455648650260.961528862271377
AZA vs. IL70.05156553280597790.787865988805980.999311006273513
AZA vs. SAHA-0.2340009975499490.3370648613304270.709111126590025
DISU vs. CD3-0.01669248038718340.9633164690900650.976860575517231
DISU vs. IL7-0.06791516049366770.7876255783332440.951780007484862
DISU vs. SAHA-0.3440001537312960.2377165275435750.616406497457998
DMSO vs. AZA-0.05142758888121720.7579152191765361
DMSO vs. CD3-0.1782533666418770.5765409730229740.676131851050219
DMSO vs. DISU-0.1640026273550380.502077943946220.909085019256221
DMSO vs. IL70.1102957843198540.5382805383620410.896696737481162
DMSO vs. SAHA-0.1896452749940230.4205616413694420.75998615072888
HIV vs. Mock in Activation0.02400776294912560.9692589967755680.999983755607037
HIV vs. Mock in Latency0.08531050019132530.6037852904381470.999834320637052
IL7 vs. CD3-0.05493747583557770.8639295407189880.913120395993079
SAHA vs. CD3-0.3743370951145810.2893656535967120.402616600471097
SAHA vs. IL7-0.2897542967779930.2335900906258730.474035679334196
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.206911 0.135775
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.977 0.907 0.843 0.728 0.916
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3Q0Q X-ray 2.0Å A=706-1056.
3Q0R X-ray 2.0Å A=706-1056.
3Q0S X-ray 2.0Å A=706-1056.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
retropepsin interacts with 22190034

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found
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