Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002383
UniProt IDQ12913
Primary gene name(s)PTPRJ
Synonym gene name(s)DEP1
Protein nameReceptor-type tyrosine-protein phosphatase eta
Protein functionTyrosine phosphatase which dephosphorylates or contributes to the dephosphorylation of CTNND1, FLT3, PDGFRB, MET, RET, variant MEN2A, KDR, LYN, SRC, MAPK1, MAPK3, EGFR, TJP1, OCLN, PIK3R1 and PIK3R2. Plays a role in cell adhesion, migration, proliferation and differentiation. Involved in vascular development. Regulator of macrophage adhesion and spreading. Positively affects cell-matrix adhesion. Positive regulator of platelet activation and thrombosis. Negative regulator of cell proliferation. Negative regulator of PDGF-stimulated cell migration; through dephosphorylation of PDGFR. Positive regulator of endothelial cell survival, as well as of VEGF-induced SRC and AKT activation; through KDR dephosphorylation. Negative regulator of EGFR signaling pathway; through EGFR dephosphorylation. Enhances the barrier function of epithelial junctions during reassembly. Negatively regulates T-cell receptor, TCR signaling. Upon T-cell TCR activation, it is up-regulated and excluded from the immunological synapses, while upon T-cell-antigen presenting cells, APC disengagement, it is no longer excluded and can dephosphorylate PLCG1 and LAT to down-regulate prolongation of signaling. {ECO:0000269|PubMed:10821867, ECO:0000269|PubMed:11259588, ECO:0000269|PubMed:12062403, ECO:0000269|PubMed:12370829, ECO:0000269|PubMed:12475979, ECO:0000269|PubMed:12913111, ECO:0000269|PubMed:14709717, ECO:0000269|PubMed:16682945, ECO:0000269|PubMed:16778204, ECO:0000269|PubMed:18348712, ECO:0000269|PubMed:18936167, ECO:0000269|PubMed:19332538, ECO:0000269|PubMed:19494114, ECO:0000269|PubMed:19836242, ECO:0000269|PubMed:19922411, ECO:0000269|PubMed:21091576, ECO:0000269|PubMed:21262971, ECO:0000269|PubMed:9531590, ECO:0000269|PubMed:9780142}.
Subcellular locationCell membrane;
Single-pass type I membrane protein. Cell projection, ruffle membrane {ECO:0000250}. Cell junction. Note=After T-cell stimulation, it is temporarily excluded from immunological synapses.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q12913
Gene Ontology
(Biological Process)
Complete annatation
contact inhibition [GO:0060242];
negative regulation of cell growth [GO:0030308];
negative regulation of cell migration [GO:0030336];
negative regulation of cell proliferation [GO:0008285];
negative regulation of epidermal growth factor receptor signaling pathway [GO:0042059];
negative regulation of MAP kinase activity [GO:0043407];
negative regulation of platelet-derived growth factor receptor signaling pathway [GO:0010642];
negative regulation of protein kinase B signaling [GO:0051898];
negative regulation of T cell receptor signaling pathway [GO:0050860];
negative regulation of vascular permeability [GO:0043116];
peptidyl-tyrosine dephosphorylation [GO:0035335];
platelet-derived growth factor receptor signaling pathway [GO:0048008];
positive chemotaxis [GO:0050918];
positive regulation of cell adhesion [GO:0045785];
positive regulation of focal adhesion assembly [GO:0051894];
positive regulation of protein kinase B signaling [GO:0051897];
regulation of cell adhesion [GO:0030155];
T cell receptor signaling pathway [GO:0050852]
Gene Ontology
(Molecular Function)
Complete annatation
beta-catenin binding [GO:0008013];
delta-catenin binding [GO:0070097];
gamma-catenin binding [GO:0045295];
mitogen-activated protein kinase binding [GO:0051019];
phosphatase activity [GO:0016791];
platelet-derived growth factor receptor binding [GO:0005161];
protein kinase binding [GO:0019901];
protein tyrosine phosphatase activity [GO:0004725]
Gene Ontology
(Cellular Component)
Complete annatation
cell-cell junction [GO:0005911];
cell surface [GO:0009986];
extracellular exosome [GO:0070062];
immunological synapse [GO:0001772];
integral component of plasma membrane [GO:0005887];
plasma membrane [GO:0005886];
ruffle membrane [GO:0032587]
Protein-protein interaction111759
Phylogenetic treeQ12913
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.2358400456047440.5906364748832040.696835455924825
AZA vs. DISU-0.4622794067087990.06819318248600240.571321267815228
AZA vs. IL7-0.1905522881371610.3223308833357220.999311006273513
AZA vs. SAHA-0.3815461967701750.1184743126625040.437982537528305
DISU vs. CD3-0.7094457339087250.1265358383080120.224347356699009
DISU vs. IL70.2620195696065560.2988025886520020.682729500927337
DISU vs. SAHA0.08255846047224110.7769489864165970.938027571439921
DMSO vs. AZA-0.04412147628568960.7924815324259561
DMSO vs. CD3-0.288441290803970.5210734116730840.627545842741627
DMSO vs. DISU0.4171031463795570.08790126495777330.546031353220793
DMSO vs. IL7-0.1396170984433250.4380189719162790.861465884101488
DMSO vs. SAHA-0.3442948889048890.1447247421349690.457647207032338
HIV vs. Mock in Activation0.6392016785388960.4933427234532280.999983755607037
HIV vs. Mock in Latency0.2384501281670520.1488057120803740.999834320637052
IL7 vs. CD3-0.4205301688772240.3381223117198890.47608758566594
SAHA vs. CD3-0.641079647436760.1431715939170540.23219807902461
SAHA vs. IL7-0.1936697253515720.4267997359810870.669188602057303
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.522585 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2CFV X-ray 2.5Å A=1019-1311.
2DLE NMR - A=366-456.
2NZ6 X-ray 2.3Å A=1019-1311.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat upregulates 20139419
HIV-1 virus replication enhanced by expression of human gene 18854154

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04520 Adherens junction - Homo sapiens (human)