Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002380
UniProt IDP08575
Primary gene name(s)PTPRC
Synonym gene name(s)CD45
Protein nameReceptor-type tyrosine-protein phosphatase C
Protein functionProtein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity, By similarity. {ECO:0000250, ECO:0000269|PubMed:11909961, ECO:0000269|PubMed:2845400}.
Subcellular locationMembrane {ECO:0000269|PubMed:12676959};
Single-pass type I membrane protein {ECO:0000269|PubMed:12676959}. Membrane raft {ECO:0000269|PubMed:12676959}. Note=Colocalized with DPP4 in membrane rafts.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P08575
Gene Ontology
(Biological Process)
Complete annatation
B cell proliferation [GO:0042100];
B cell receptor signaling pathway [GO:0050853];
bone marrow development [GO:0048539];
cell cycle phase transition [GO:0044770];
cell surface receptor signaling pathway [GO:0007166];
defense response to virus [GO:0051607];
dephosphorylation [GO:0016311];
hematopoietic progenitor cell differentiation [GO:0002244];
immunoglobulin biosynthetic process [GO:0002378];
negative regulation of cell adhesion involved in substrate-bound cell migration [GO:0006933];
negative regulation of cytokine-mediated signaling pathway [GO:0001960];
negative regulation of protein kinase activity [GO:0006469];
negative regulation of T cell mediated cytotoxicity [GO:0001915];
positive regulation of antigen receptor-mediated signaling pathway [GO:0050857];
positive regulation of B cell proliferation [GO:0030890];
positive regulation of hematopoietic stem cell migration [GO:2000473];
positive regulation of protein kinase activity [GO:0045860];
positive regulation of stem cell proliferation [GO:2000648];
positive regulation of T cell proliferation [GO:0042102];
protein dephosphorylation [GO:0006470];
regulation of cell cycle [GO:0051726];
release of sequestered calcium ion into cytosol [GO:0051209];
stem cell development [GO:0048864];
T cell differentiation [GO:0030217];
T cell receptor signaling pathway [GO:0050852]
Gene Ontology
(Molecular Function)
Complete annatation
protein kinase binding [GO:0019901];
protein tyrosine phosphatase activity [GO:0004725];
transmembrane receptor protein tyrosine phosphatase activity [GO:0005001]
Gene Ontology
(Cellular Component)
Complete annatation
cell surface [GO:0009986];
external side of plasma membrane [GO:0009897];
extracellular exosome [GO:0070062];
focal adhesion [GO:0005925];
integral component of plasma membrane [GO:0005887];
intracellular [GO:0005622];
membrane [GO:0016020];
membrane raft [GO:0045121];
plasma membrane [GO:0005886]
Protein-protein interaction111752
Phylogenetic treeP08575
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-2.090571170269661.15120024624105e-091.63842210683145e-08
AZA vs. DISU-0.07111025622305060.7780216105902730.976444104025318
AZA vs. IL7-0.1284578007225080.5022492593204610.999311006273513
AZA vs. SAHA-0.3573007918408360.1446834861295030.484384314018191
DISU vs. CD32.006707214669991.08893463690585e-071.34106215005872e-06
DISU vs. IL7-0.06636823886719350.791574310871390.952725938130142
DISU vs. SAHA-0.2850246417076660.3288298278545090.710382093644071
DMSO vs. AZA-0.004432839704838960.9787621959254851
DMSO vs. CD32.074542025643515.98851856992155e-108.06229063948777e-09
DMSO vs. DISU0.06481278783292130.7899435082754640.973837440160022
DMSO vs. IL7-0.1168291488115880.5140045947576050.887822730765496
DMSO vs. SAHA-0.3596279774969850.1286298271537150.429269292522949
HIV vs. Mock in Activation-0.216178963233930.7289762017704610.999983755607037
HIV vs. Mock in Latency0.1055304424309320.520160045699570.999834320637052
IL7 vs. CD31.970246799943263.81093478996064e-096.14355295070339e-08
SAHA vs. CD31.708646465577562.45535574039391e-061.81739739723655e-05
SAHA vs. IL7-0.2324940249400080.3399905194375080.589692450811298
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.58044 0.000839432
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.98 1.429 1.677 1.804 1.708
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1YGR X-ray 2.9Å A/B=622-1231.
1YGU X-ray 2.9Å A/B=622-1231.
5FMV X-ray 2.9Å A/B=223-571.
5FN6 X-ray 3.3Å A=223-479.
5FN7 X-ray 2.3Å A/B=223-392.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp120 downregulates 24162774
Envelope surface glycoprotein gp120 interacts with 11804554
Envelope surface glycoprotein gp120 modulated by 16524887
Envelope surface glycoprotein gp120 regulated by 16524887
Nef upregulates 20156100
Tat interacts with 22937208

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04514 Cell adhesion molecules (CAMs) - Homo sapiens (human)
hsa04660 T cell receptor signaling pathway - Homo sapiens (human)
hsa04666 Fc gamma R-mediated phagocytosis - Homo sapiens (human)
hsa05340 Primary immunodeficiency - Homo sapiens (human)