Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002370
UniProt IDP35408
Primary gene name(s)PTGER4
Synonym gene name(s)PTGER2
Protein nameProstaglandin E2 receptor EP4 subtype
Protein functionReceptor for prostaglandin E2, PGE2. The activity of this receptor is mediated by G(s proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.
Subcellular locationCell membrane;
Multi-pass membrane protein.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P35408
Gene Ontology
(Biological Process)
Complete annatation
adenylate cyclase-activating G-protein coupled receptor signaling pathway [GO:0007189];
adenylate cyclase-modulating G-protein coupled receptor signaling pathway [GO:0007188];
bone development [GO:0060348];
cellular response to mechanical stimulus [GO:0071260];
cellular response to prostaglandin E stimulus [GO:0071380];
ERK1 and ERK2 cascade [GO:0070371];
immune response [GO:0006955];
JNK cascade [GO:0007254];
negative regulation of cytokine secretion [GO:0050710];
negative regulation of eosinophil extravasation [GO:2000420];
negative regulation of inflammatory response [GO:0050728];
negative regulation of integrin activation [GO:0033624];
positive regulation of cytokine secretion [GO:0050715];
positive regulation of cytosolic calcium ion concentration [GO:0007204];
positive regulation of inflammatory response [GO:0050729];
regulation of ossification [GO:0030278];
regulation of stress fiber assembly [GO:0051492];
response to lipopolysaccharide [GO:0032496];
response to mechanical stimulus [GO:0009612];
T-helper cell differentiation [GO:0042093]
Gene Ontology
(Molecular Function)
Complete annatation
prostaglandin E receptor activity [GO:0004957]
Gene Ontology
(Cellular Component)
Complete annatation
integral component of membrane [GO:0016021];
neuronal cell body [GO:0043025];
neuron projection terminus [GO:0044306];
nuclear membrane [GO:0031965];
plasma membrane [GO:0005886]
Protein-protein interaction111706
Phylogenetic treeP35408
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.07799619514080.001134141899947320.004058353921179
AZA vs. DISU-0.4729804314309020.06197154698642290.553084132192622
AZA vs. IL7-0.02102727490756280.9127816501626080.999311006273513
AZA vs. SAHA-0.04785413143389970.8441790681508680.963078428313493
DISU vs. CD30.594030779921830.1019255629170840.189191717203807
DISU vs. IL70.4421158359516790.08068119458517840.376437328496306
DISU vs. SAHA0.4273672958570.142975614595460.485968761291876
DMSO vs. AZA-0.1953379186895760.2425380059859091
DMSO vs. CD30.874724341979110.006654573324926140.0175172369631334
DMSO vs. DISU0.2768357273715170.256862230542750.7748026049398
DMSO vs. IL70.1809778129490760.3135866988261580.795175691304346
DMSO vs. SAHA0.141094203321450.5488512904608620.837983770492923
HIV vs. Mock in Activation-0.2683784554945020.6663313723716560.999983755607037
HIV vs. Mock in Latency0.05189496436990.7824700771856750.999834320637052
IL7 vs. CD31.063157791150390.001111113306529690.0046457173007828
SAHA vs. CD31.007112951441110.004751797931741190.0136816191882575
SAHA vs. IL7-0.02909504011587260.9048465922764540.963933394485885
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
0.2958 0.0395

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock 0.824 6.37E-13 4.66E-10
Infected vs. Bystander 0.957 2.56E-14 1.98E-11
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0456983 0.807492
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
204896_s_at 2.09 No downregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00917 Dinoprostone approved yes agonist
DB00929 Misoprostol approved unknown agonist

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
capsid downregulated by 24586238

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04080 Neuroactive ligand-receptor interaction - Homo sapiens (human)
hsa04750 Inflammatory mediator regulation of TRP channels - Homo sapiens (human)
hsa04924 Renin secretion - Homo sapiens (human)
hsa05200 Pathways in cancer - Homo sapiens (human)