Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002362
UniProt IDQ8WXF1
Primary gene name(s)PSPC1
Synonym gene name(s)PSP1
Protein nameParaspeckle component 1
Protein functionRegulates, cooperatively with NONO and SFPQ, androgen receptor-mediated gene transcription activity in Sertoli cell line, By similarity. Binds to poly(A, poly(G and poly(U RNA homopolymers. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer, By similarity. Together with NONO, required for the formation of nuclear paraspeckles. {ECO:0000250, ECO:0000269|PubMed:22416126}.
Subcellular locationNucleus, nucleolus. Nucleus matrix {ECO:0000250}. Cytoplasm {ECO:0000250}. Nucleus speckle. Note=In punctate subnuclear structures often located adjacent to splicing speckles, called paraspeckles. Colocalizes with NONO and SFPQ in paraspeckles and perinucleolar caps in an RNA-dependent manner. May cycles between paraspeckles and nucleolus. In telophase, when daughter nuclei form, localizes to perinucleolar caps.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q8WXF1
Gene Ontology
(Biological Process)
Complete annatation
mRNA splicing, via spliceosome [GO:0000398];
negative regulation of transcription, DNA-templated [GO:0045892];
regulation of circadian rhythm [GO:0042752];
regulation of transcription, DNA-templated [GO:0006355];
rhythmic process [GO:0048511];
transcription, DNA-templated [GO:0006351]
Gene Ontology
(Molecular Function)
Complete annatation
core promoter binding [GO:0001047];
nucleotide binding [GO:0000166];
poly(A RNA binding [GO:0044822];
transcription regulatory region sequence-specific DNA binding [GO:0000976]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
nuclear matrix [GO:0016363];
nuclear speck [GO:0016607];
nucleolus [GO:0005730];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
paraspeckles [GO:0042382]
Protein-protein interaction120558
Phylogenetic treeQ8WXF1
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.102117426831530.0008797593272800160.00324516215877739
AZA vs. DISU-0.02998294329753060.9058596404788020.994169411479968
AZA vs. IL70.05984692661005310.756807681566770.999311006273513
AZA vs. SAHA-0.5138798586505640.03663450834945030.224302516309604
DISU vs. CD3-1.145268761006380.001800337696475360.00685656655802668
DISU vs. IL70.0804474905621860.7499388350181170.939560944033259
DISU vs. SAHA-0.4820452122335610.09997291830585490.404339262293689
DMSO vs. AZA0.01411701406474230.9333562209743061
DMSO vs. CD3-1.097867530133330.0006870270309309310.00246078512148704
DMSO vs. DISU0.04283670078553320.8609404274753130.984640346119887
DMSO vs. IL70.05264283995858070.7709052628344080.953428432386045
DMSO vs. SAHA-0.534620492774990.02439168917188710.161656352591629
HIV vs. Mock in Activation0.1741811921033480.7794745475338260.999983755607037
HIV vs. Mock in Latency0.06083985137893490.7141888869286320.999834320637052
IL7 vs. CD3-1.035565420190370.001419883596965540.0057454779750501
SAHA vs. CD3-1.639961378624935.97787360412383e-063.99851554434736e-05
SAHA vs. IL7-0.5766841127527720.01881989859242360.0966579732136946
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change -1.284925789
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.225854 0.109886
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.05 0.882 0.818 0.846 0.855
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3SDE X-ray 1.9Å A=61-320.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Rev interacts with 22174317

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found