Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002352
UniProt IDP60900
Primary gene name(s)PSMA6
Synonym gene name(s)PROS27
Protein nameProteasome subunit alpha type-6
Protein functionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Subcellular locationCytoplasm. Nucleus. Cytoplasm, P-body {ECO:0000250}. Note=Colocalizes with TRIM5 in the cytoplasmic bodies. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P60900
Gene Ontology
(Biological Process)
Complete annatation
anaphase-promoting complex-dependent catabolic process [GO:0031145];
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent [GO:0002479];
Fc-epsilon receptor signaling pathway [GO:0038095];
MAPK cascade [GO:0000165];
negative regulation of canonical Wnt signaling pathway [GO:0090090];
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [GO:0051436];
NIK/NF-kappaB signaling [GO:0038061];
positive regulation of canonical Wnt signaling pathway [GO:0090263];
positive regulation of NF-kappaB transcription factor activity [GO:0051092];
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition [GO:0051437];
proteasome-mediated ubiquitin-dependent protein catabolic process [GO:0043161];
protein polyubiquitination [GO:0000209];
proteolysis involved in cellular protein catabolic process [GO:0051603];
regulation of cellular amino acid metabolic process [GO:0006521];
regulation of inflammatory response [GO:0050727];
regulation of mRNA stability [GO:0043488];
stimulatory C-type lectin receptor signaling pathway [GO:0002223];
T cell receptor signaling pathway [GO:0050852];
tumor necrosis factor-mediated signaling pathway [GO:0033209];
Wnt signaling pathway, planar cell polarity pathway [GO:0060071]
Gene Ontology
(Molecular Function)
Complete annatation
endopeptidase activity [GO:0004175];
NF-kappaB binding [GO:0051059];
purine ribonucleoside triphosphate binding [GO:0035639];
RNA binding [GO:0003723];
threonine-type endopeptidase activity [GO:0004298]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytoplasmic mRNA processing body [GO:0000932];
cytosol [GO:0005829];
extracellular exosome [GO:0070062];
myofibril [GO:0030016];
nuclear matrix [GO:0016363];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
polysome [GO:0005844];
proteasome complex [GO:0000502];
proteasome core complex [GO:0005839];
proteasome core complex, alpha-subunit complex [GO:0019773];
sarcomere [GO:0030017]
Protein-protein interaction111660
Phylogenetic treeP60900
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD32.169360097783922.25826579658417e-103.68361277988275e-09
AZA vs. DISU0.4087347776308450.1087612221789140.653579050610225
AZA vs. IL70.4332900443300260.02488863090897510.505422034167827
AZA vs. SAHA-0.08485951197598460.7289435612355570.924705766491882
DISU vs. CD3-1.774759111952682.56590642311316e-062.21780237511513e-05
DISU vs. IL70.01576506802430490.9503674503198080.991194708338029
DISU vs. SAHA-0.4919775170459610.09256217675157210.388872466110566
DMSO vs. AZA-0.0958264898970650.5698062550000571
DMSO vs. CD3-2.276477194847231.14465104061878e-112.09783064804403e-10
DMSO vs. DISU-0.5066176407740320.0393569750783210.398866336139429
DMSO vs. IL70.5363654683839340.003026095539071410.106559075697104
DMSO vs. SAHA0.003889251655620160.9868830564683380.9961149026161
HIV vs. Mock in Activation-0.2481113472461160.6898291706528490.999983755607037
HIV vs. Mock in Latency-0.04810795736668530.7725199971020190.999834320637052
IL7 vs. CD3-1.727287513916631.73572567430114e-072.01560809620501e-06
SAHA vs. CD3-2.278962448074199.04467167828216e-101.51112197551787e-08
SAHA vs. IL7-0.5213771453414550.0332336413478320.139660972867917
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
1.7 1.56E-05 1.3 0.005064337 1.4 0.008637005
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.118434 0.563337
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.017 0.964 1.078 1.087 0.971
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
208805_at 1.6 No upregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB08515 (3AR,6R,6AS)-6-((S)-((S)-CYCLOHEX-2-ENYL)(HYDROXY)METHYL)-6A-METHYL-4-OXO-HEXAHYDRO-2H-FURO[3,2-C]PYRROLE-6-CARBALDEHYDE experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4R3O X-ray 2.6Å A/O=2-245.
4R67 X-ray 2.8Å A/O/c/q=2-245.
5A0Q EM 3.5Å A/O=1-246.
5GJQ EM 4.5Å B/h=1-246.
5GJR EM 3.5Å B/h=1-246.
5L4G EM 4.0Å A/N=1-246.
5LE5 X-ray 1.8Å G/U=1-246.
5LEX X-ray 2.2Å G/U=1-246.
5LEY X-ray 1.9Å G/U=1-246.
5LEZ X-ray 2.1Å G/U=1-246.
5LF0 X-ray 2.4Å G/U=1-246.
5LF1 X-ray 2.0Å G/U=1-246.
5LF3 X-ray 2.1Å G/U=1-246.
5LF4 X-ray 1.9Å G/U=1-246.
5LF6 X-ray 2.0Å G/U=1-246.
5LF7 X-ray 2.0Å G/U=1-246.
5T0C EM 3.8Å AG/BG=2-246.
5T0G EM 4.4Å G=2-246.
5T0H EM 6.8Å G=2-246.
5T0I EM 8.0Å G=2-246.
5T0J EM 8.0Å G=2-246.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03050 Proteasome - Homo sapiens (human)