Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002338
UniProt IDQ6P2Q9
Primary gene name(s)PRPF8
Synonym gene name(s)PRPC8
Protein namePre-mRNA-processing-splicing factor 8
Protein functionFunctions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex. Functions as scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site. {ECO:0000269|PubMed:20595234, ECO:0000303|PubMed:15840809}.
Subcellular locationNucleus speckle {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q6P2Q9
Gene Ontology
(Biological Process)
Complete annatation
cellular response to lipopolysaccharide [GO:0071222];
cellular response to tumor necrosis factor [GO:0071356];
mRNA processing [GO:0006397];
mRNA splicing, via spliceosome [GO:0000398];
RNA splicing [GO:0008380];
RNA splicing, via transesterification reactions [GO:0000375];
spliceosomal tri-snRNP complex assembly [GO:0000244]
Gene Ontology
(Molecular Function)
Complete annatation
K63-linked polyubiquitin binding [GO:0070530];
poly(A RNA binding [GO:0044822];
pre-mRNA intronic binding [GO:0097157];
second spliceosomal transesterification activity [GO:0000386];
U1 snRNA binding [GO:0030619];
U2 snRNA binding [GO:0030620];
U5 snRNA binding [GO:0030623];
U6 snRNA binding [GO:0017070]
Gene Ontology
(Cellular Component)
Complete annatation
catalytic step 2 spliceosome [GO:0071013];
membrane [GO:0016020];
nuclear speck [GO:0016607];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
U5 snRNP [GO:0005682]
Protein-protein interaction115842
Phylogenetic treeQ6P2Q9
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.3542857332563310.2787879667979350.394415805651653
AZA vs. DISU0.2013913252977210.4251056364903160.913479265604449
AZA vs. IL70.1188138050703370.5351481532756310.999311006273513
AZA vs. SAHA-0.07527532257714550.7571457882966330.934207711613608
DISU vs. CD3-0.1653995935961250.6478803689061960.749340035363461
DISU vs. IL7-0.09187706898484970.7146233392071550.927165784358565
DISU vs. SAHA-0.2745747990981110.3452400670863360.723056288362726
DMSO vs. AZA0.1107875638596730.506608172273251
DMSO vs. CD3-0.2554101925676820.4240902354687550.538467711532811
DMSO vs. DISU-0.09262701612695980.7034546419936770.95973394047213
DMSO vs. IL70.01545478157510310.931231801570210.98672467966686
DMSO vs. SAHA-0.1918875964183510.4145052407285760.755169345882542
HIV vs. Mock in Activation0.2950467312715020.6358759581275710.999983755607037
HIV vs. Mock in Latency-0.01682813589911460.9184255644256460.999834320637052
IL7 vs. CD3-0.2290653270678680.4753252914374850.60727056431914
SAHA vs. CD3-0.4535073992244260.19968499560990.301770245070316
SAHA vs. IL7-0.1969751489297170.4174748141991760.661795703989733
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.247491 0.0619576
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.995 0.989 0.925 0.877 1.011
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
200000_s_at 1.36 No downregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3E9L X-ray 1.9Å A=1760-2016.
3ENB X-ray 1.8Å A/B=1769-1990.
3JCR EM 7.0Å A=1-2335.
3LRU X-ray 1.8Å A/B=1831-1990.
4JK7 X-ray 1.4Å A/B=1769-1990.
4JK8 X-ray 1.1Å A/B=1769-1990.
4JK9 X-ray 1.5Å A/B=1769-1990.
4JKA X-ray 1.3Å A/B=1769-1990.
4JKB X-ray 1.3Å A/B=1769-1990.
4JKC X-ray 1.5Å A/B=1769-1990.
4JKD X-ray 1.5Å A/B=1769-1990.
4JKE X-ray 1.6Å A/B=1769-1990.
4JKF X-ray 1.9Å A/B=1769-1990.
4JKG X-ray 1.8Å A/B=1769-1990.
4JKH X-ray 1.8Å A/B=1769-1990.
4KIT X-ray 3.6Å C=2064-2335.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Rev interacts with 22174317
Envelope surface glycoprotein gp120 complexes with 23125841
Gag-Pol complexes with 23125841
Pr55(Gag) complexes with 23125841
HIV-1 virus replication enhanced by expression of human gene 18854154
Tat interacts with 19454010
Nef complexes with 23125841

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03040 Spliceosome - Homo sapiens (human)