Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002328
UniProt IDQ96LA8
Primary gene name(s)PRMT6
Synonym gene name(s)HRMT1L6
Protein nameProtein arginine N-methyltransferase 6
Protein functionArginine methyltransferase that can catalyze the formation of both omega-N monomethylarginine, MMA and asymmetrical dimethylarginine, aDMA, with a strong preference for the formation of aDMA. Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates. Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a. H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 'Lys-4', H3K4me2 and H3K4me3. Acts as a transcriptional repressor of various genes such as HOXA2, THBS1 and TP53. Repression of TP53 blocks cellular senescence, By similarity. Also methylates histone H2A and H4 'Arg-3', H2AR3me and H4R3me, respectively. Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta, POLB, leading to the stimulation of its polymerase activity by enhancing DNA binding and processivity. Methylates HMGA1. Regulates alternative splicing events. Acts as a transcriptional coactivator of a number of steroid hormone receptors including ESR1, ESR2, PGR and NR3C1. Promotes fasting-induced transcriptional activation of the gluconeogenic program through methylation of the CRTC2 transcription coactivator. May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7, NC. {ECO:0000250, ECO:0000269|PubMed:11724789, ECO:0000269|PubMed:16157300, ECO:0000269|PubMed:16159886, ECO:0000269|PubMed:16600869, ECO:0000269|PubMed:17267505, ECO:0000269|PubMed:17898714, ECO:0000269|PubMed:18077460, ECO:0000269|PubMed:18079182, ECO:0000269|PubMed:19405910, ECO:0000269|PubMed:19509293, ECO:0000269|PubMed:20047962}.
Subcellular locationNucleus {ECO:0000269|PubMed:11724789}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q96LA8
Gene Ontology
(Biological Process)
Complete annatation
base-excision repair [GO:0006284];
cell growth [GO:0016049];
cellular senescence [GO:0090398];
histone H3-R2 methylation [GO:0034970];
histone methylation [GO:0016571];
negative regulation of transcription, DNA-templated [GO:0045892];
negative regulation of transcription from RNA polymerase II promoter [GO:0000122];
peptidyl-arginine methylation, to asymmetrical-dimethyl arginine [GO:0019919];
transcription, DNA-templated [GO:0006351];
viral process [GO:0016032]
Gene Ontology
(Molecular Function)
Complete annatation
chromatin binding [GO:0003682];
histone-arginine N-methyltransferase activity [GO:0008469];
histone binding [GO:0042393];
histone methyltransferase activity [GO:0042054];
histone methyltransferase activity, H2A-R3 specific [GO:0070612];
histone methyltransferase activity, H3-R2 specific [GO:0070611];
histone methyltransferase activity, H4-R3 specific [GO:0044020];
protein-arginine N-methyltransferase activity [GO:0016274];
protein-arginine omega-N asymmetric methyltransferase activity [GO:0035242];
protein-arginine omega-N monomethyltransferase activity [GO:0035241]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction120469
Phylogenetic treeQ96LA8
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.8914427363607330.007161397132916970.019642689278858
AZA vs. DISU-0.7932702029206580.002165482939011980.117427545646417
AZA vs. IL70.1462722954038920.4549678940130820.999311006273513
AZA vs. SAHA-0.06153226343529830.8032137249228490.948942600854645
DISU vs. CD3-1.696877973243276.51386967487344e-065.10343406855729e-05
DISU vs. IL70.9301183342024810.0003052452770149210.0121734723571427
DISU vs. SAHA0.7327868030409860.01355640232072060.126638573484536
DMSO vs. AZA-0.04270734284760690.8039434228895981
DMSO vs. CD3-0.9457834468437970.003526312429049660.0102161339891602
DMSO vs. DISU0.7485289718969930.002664979340846530.112182230912171
DMSO vs. IL70.1962374106600260.2843227017789330.774064325587485
DMSO vs. SAHA-0.02546948769602110.9149777242873020.979615288900962
HIV vs. Mock in Activation0.06118650505110610.9217694774059890.999983755607037
HIV vs. Mock in Latency-0.1027176757617490.5442983601237950.999834320637052
IL7 vs. CD3-0.7374248810705950.02300022516389340.0585216735137349
SAHA vs. CD3-0.9775019516711890.0063055135146860.0173877563890792
SAHA vs. IL7-0.21147680936190.3903130143695770.63938039324136
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.040604 0.830816
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.928 0.954 0.89 0.876 0.982
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4HC4 X-ray 1.9Å A=1-375.
4QPP X-ray 2.5Å A/B/C=1-375.
4QQK X-ray 1.8Å A=1-375.
4Y2H X-ray 2.3Å A/B=27-375.
4Y30 X-ray 2.1Å A/B=25-375.
5E8R X-ray 2.5Å A/B=1-375.
5EGS X-ray 2.1Å A/B/C/D=1-375.
5HZM X-ray 2.0Å A=1-375.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat methylated by 15596808
Tat upregulates 19726520
Rev methylated by 17176473
nucleocapsid methylated by 17415034
Tat interacts with 23800116
Envelope surface glycoprotein gp160; precursor methylated by 17169163

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found