Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002327
UniProt IDO14744
Primary gene name(s)PRMT5
Synonym gene name(s)HRMT1L5, IBP72, JBP1, SKB1
Protein nameProtein arginine N-methyltransferase 5
Protein functionArginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine, MMA and symmetrical dimethylarginine, sDMA, with a preference for the formation of MMA, PubMed:10531356, PubMed:11152681, PubMed:11747828, PubMed:12411503, PubMed:15737618, PubMed:17709427, PubMed:20159986, PubMed:20810653, PubMed:21258366, PubMed:21917714, PubMed:22269951. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1, SNRPD1 and Sm D3, SNRPD3; such methylation being required for the assembly and biogenesis of snRNP core particles, PubMed:12411503, PubMed:11747828, PubMed:17709427. Methylates SUPT5H and may regulate its transcriptional elongation properties, PubMed:12718890. Mono- and dimethylates arginine residues of myelin basic protein, MBP in vitro. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins, PIWIL1, PIWIL2 and PIWIL4, methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage, By similarity. Methylates RPS10. Attenuates EGF signaling through the MAPK1/MAPK3 pathway acting at 2 levels. First, monomethylates EGFR; this enhances EGFR 'Tyr-1197' phosphorylation and PTPN6 recruitment, eventually leading to reduced SOS1 phosphorylation, PubMed:21917714, PubMed:21258366. Second, methylates RAF1 and probably BRAF, hence destabilizing these 2 signaling proteins and reducing their catalytic activity, PubMed:21917714. Required for induction of E-selectin and VCAM-1, on the endothelial cells surface at sites of inflammation. Methylates HOXA9, PubMed:22269951. Methylates and regulates SRGAP2 which is involved in cell migration and differentiation, PubMed:20810653. Acts as a transcriptional corepressor in CRY1-mediated repression of the core circadian component PER1 by regulating the H4R3 dimethylation at the PER1 promoter, By similarity. Methylates GM130/GOLGA2, regulating Golgi ribbon formation, PubMed:20421892. Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP- and/or TET1-dependent manner, PubMed:25284789. Symmetrically methylates POLR2A, a modification that allows the recruitment to POLR2A of proteins including SMN1/SMN2 and SETX. This is required for resolving RNA-DNA hybrids created by RNA polymerase II, that form R-loop in transcription terminal regions, an important step in proper transcription termination, PubMed:26700805. {ECO:0000250|UniProtKB:Q8CIG8, ECO:0000269|PubMed:10531356, ECO:0000269|PubMed:11152681, ECO:0000269|PubMed:11747828, ECO:0000269|PubMed:12411503, ECO:0000269|PubMed:12718890, ECO:0000269|PubMed:15737618, ECO:0000269|PubMed:17709427, ECO:0000269|PubMed:20159986, ECO:0000269|PubMed:20421892, ECO:0000269|PubMed:20810653, ECO:0000269|PubMed:21258366, ECO:0000269|PubMed:21917714, ECO:0000269|PubMed:22269951, ECO:0000269|PubMed:25284789, ECO:0000269|PubMed:26700805}.
Subcellular locationCytoplasm {ECO:0000269|PubMed:21917714, ECO:0000269|PubMed:22269951}. Nucleus {ECO:0000269|PubMed:18404153, ECO:0000269|PubMed:21917714, ECO:0000269|PubMed:22269951}. Golgi apparatus {ECO:0000269|PubMed:20421892}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O14744
Gene Ontology
(Biological Process)
Complete annatation
cell proliferation [GO:0008283];
circadian regulation of gene expression [GO:0032922];
DNA-templated transcription, termination [GO:0006353];
endothelial cell activation [GO:0042118];
Golgi ribbon formation [GO:0090161];
histone H4-R3 methylation [GO:0043985];
liver regeneration [GO:0097421];
negative regulation of cell differentiation [GO:0045596];
peptidyl-arginine methylation [GO:0018216];
peptidyl-arginine N-methylation [GO:0035246];
positive regulation of adenylate cyclase-inhibiting dopamine receptor signaling pathway [GO:1904992];
positive regulation of oligodendrocyte differentiation [GO:0048714];
regulation of DNA methylation [GO:0044030];
regulation of ERK1 and ERK2 cascade [GO:0070372];
regulation of mitotic nuclear division [GO:0007088];
regulation of signal transduction by p53 class mediator [GO:1901796];
regulation of transcription, DNA-templated [GO:0006355];
spliceosomal snRNP assembly [GO:0000387]
Gene Ontology
(Molecular Function)
Complete annatation
core promoter sequence-specific DNA binding [GO:0001046];
histone-arginine N-methyltransferase activity [GO:0008469];
histone methyltransferase activity, H4-R3 specific [GO:0044020];
methyl-CpG binding [GO:0008327];
methyltransferase activity [GO:0008168];
protein-arginine N-methyltransferase activity [GO:0016274];
protein-arginine omega-N symmetric methyltransferase activity [GO:0035243];
protein heterodimerization activity [GO:0046982];
ribonucleoprotein complex binding [GO:0043021];
transcription corepressor activity [GO:0003714]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
Golgi apparatus [GO:0005794];
histone methyltransferase complex [GO:0035097];
methylosome [GO:0034709];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction115688
Phylogenetic treeO14744
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.949010500518889.02082275544558e-091.06972337191248e-07
AZA vs. DISU-0.4295408527286340.09107544581859210.622680215201834
AZA vs. IL70.3810854280203440.04862401269481120.647245692416997
AZA vs. SAHA-0.004685688969968680.9847298021505510.996858691786281
DISU vs. CD3-2.39089491302063.48809536809824e-107.81271167588602e-09
DISU vs. IL70.8012763422300610.001605582935577440.0372371734674307
DISU vs. SAHA0.4268155183089820.1444032449284660.488507587949925
DMSO vs. AZA0.05945287352811960.7245032089219151
DMSO vs. CD3-1.902045281643168.74980676801584e-099.46273072219373e-08
DMSO vs. DISU0.4867262113913210.04721255312139410.432781736946112
DMSO vs. IL70.3291713012084970.0684616256569250.500028588555303
DMSO vs. SAHA-0.06979129247714570.7679687508730980.933692888384032
HIV vs. Mock in Activation0.05367102798354230.9311770093434490.999983755607037
HIV vs. Mock in Latency-0.03779346326537870.8207094199487840.999834320637052
IL7 vs. CD3-1.561186922290992.02369540747149e-061.83864743079071e-05
SAHA vs. CD3-1.977641672639526.4221821305388e-087.10354926607343e-07
SAHA vs. IL7-0.3883115659803990.1121741369348650.308170209754152
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change -1.326384428
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.546733 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.965 0.972 0.937 0.891 0.927
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4GQB X-ray 2.0Å A=1-637.
4X60 X-ray 2.3Å A=2-637.
4X61 X-ray 2.8Å A=2-637.
4X63 X-ray 3.0Å A=2-637.
5C9Z X-ray 2.3Å A=2-637.
5EMJ X-ray 2.2Å A=2-637.
5EMK X-ray 2.5Å A=2-637.
5EML X-ray 2.3Å A=2-637.
5EMM X-ray 2.3Å A=2-637.
5FA5 X-ray 2.3Å A=1-637.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03013 RNA transport - Homo sapiens (human)