Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002325
UniProt IDQ99873
Primary gene name(s)PRMT1
Synonym gene name(s)HMT2, HRMT1L2, IR1B4
Protein nameProtein arginine N-methyltransferase 1
Protein functionArginine methyltransferase that methylates, mono and asymmetric dimethylation the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4', H4R3me1 and H4R3me2a, respectively, a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma, IFN-gamma signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor, ER-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Methylates FOXO1 and retains it in the nucleus increasing its transcriptional activity. Methylates CHTOP and this methylation is critical for its 5-hydroxymethylcytosine, 5hmC-binding activity, PubMed:25284789. Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP- and/or TET1-dependent manner, PubMed:25284789. {ECO:0000269|PubMed:11387442, ECO:0000269|PubMed:11448779, ECO:0000269|PubMed:12718890, ECO:0000269|PubMed:18320585, ECO:0000269|PubMed:18657504, ECO:0000269|PubMed:18773938, ECO:0000269|PubMed:19124016, ECO:0000269|PubMed:19136629, ECO:0000269|PubMed:19405910, ECO:0000269|PubMed:20442406, ECO:0000269|PubMed:25284789}.
Subcellular locationNucleus {ECO:0000269|PubMed:19136629}. Nucleus, nucleoplasm {ECO:0000250}. Cytoplasm, cytosol {ECO:0000250}. Note=Mostly found in the cytoplasm. Colocalizes with CHTOP within the nucleus. Low levels detected also in the chromatin fraction, By similarity. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q99873
Gene Ontology
(Biological Process)
Complete annatation
cell surface receptor signaling pathway [GO:0007166];
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest [GO:0006977];
histone H4-R3 methylation [GO:0043985];
histone methylation [GO:0016571];
negative regulation of megakaryocyte differentiation [GO:0045653];
neuron projection development [GO:0031175];
peptidyl-arginine methylation [GO:0018216];
positive regulation of erythrocyte differentiation [GO:0045648];
positive regulation of hemoglobin biosynthetic process [GO:0046985];
positive regulation of p38MAPK cascade [GO:1900745];
protein methylation [GO:0006479];
regulation of transcription, DNA-templated [GO:0006355]
Gene Ontology
(Molecular Function)
Complete annatation
enzyme binding [GO:0019899];
histone methyltransferase activity [GO:0042054];
histone methyltransferase activity, H4-R3 specific [GO:0044020];
identical protein binding [GO:0042802];
methyl-CpG binding [GO:0008327];
methyltransferase activity [GO:0008168];
mitogen-activated protein kinase p38 binding [GO:0048273];
N-methyltransferase activity [GO:0008170];
poly(A RNA binding [GO:0044822];
protein-arginine N-methyltransferase activity [GO:0016274];
protein-arginine omega-N asymmetric methyltransferase activity [GO:0035242];
protein methyltransferase activity [GO:0008276]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
methylosome [GO:0034709];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction109512
Phylogenetic treeQ99873
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD32.24032946997835.79877257322892e-111.06066869203527e-09
AZA vs. DISU-0.1890708135378050.4551297416869290.921997150117399
AZA vs. IL70.4202284062052950.02916595921952090.54046606875835
AZA vs. SAHA0.2645828499242180.2785863955337540.652061239758866
DISU vs. CD3-2.441442294143531.61009872101658e-103.79228564384437e-09
DISU vs. IL70.6000765866549830.01759750060752470.165491155120671
DISU vs. SAHA0.45569202184770.1188475478138970.442844558472533
DMSO vs. AZA0.1032525424906390.5384713754883131
DMSO vs. CD3-2.151878737298541.05156106045001e-101.64570680378486e-09
DMSO vs. DISU0.289462767677550.2358676438908150.752917897359581
DMSO vs. IL70.3248832956757210.0711674817714590.510394761039365
DMSO vs. SAHA0.1565316686086230.507108470612730.818074429013708
HIV vs. Mock in Activation0.02531041458326970.9675339785050450.999983755607037
HIV vs. Mock in Latency-0.1236825976106180.4545911002709710.999834320637052
IL7 vs. CD3-1.813321380569294.18717229777599e-085.58524318976792e-07
SAHA vs. CD3-1.999416893323064.62284603930385e-085.2857579523755e-07
SAHA vs. IL7-0.1581962135733270.5159470382315090.740109569118881
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change -1.381383696
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.357865 0.0779518
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.956 1.135 1.233 1.167 1.209
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB01752 S-Adenosyl-L-Homocysteine experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
nucleocapsid interacts with 22190034
Pr55(Gag) interacts with 22190034

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04068 FoxO signaling pathway - Homo sapiens (human)
hsa04922 Glucagon signaling pathway - Homo sapiens (human)
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