Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002311
UniProt IDP48147
Primary gene name(s)PREP
Synonym gene name(s)PEP
Protein nameProlyl endopeptidase
Protein functionCleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long.
Subcellular locationCytoplasm.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P48147
Gene Ontology
(Biological Process)
Complete annatation
proteolysis [GO:0006508]
Gene Ontology
(Molecular Function)
Complete annatation
serine-type endopeptidase activity [GO:0004252];
serine-type exopeptidase activity [GO:0070008];
serine-type peptidase activity [GO:0008236]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
membrane [GO:0016020];
nucleus [GO:0005634]
Protein-protein interaction111541
Phylogenetic treeP48147
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.8348288771678830.01130680332536740.0289527930207165
AZA vs. DISU-0.1889362707964690.4566203537335860.921997150117399
AZA vs. IL70.211916977584810.2728118919907310.999311006273513
AZA vs. SAHA-0.03573288430890560.8838604310213070.974760742725938
DISU vs. CD3-1.035472997920250.004703796724128130.0153840381469966
DISU vs. IL70.3915948457188940.1212580911136460.455930760316363
DISU vs. SAHA0.1546867153315140.5963510823847350.87077972440623
DMSO vs. AZA0.005019631366333340.9762716882662291
DMSO vs. CD3-0.8407200034485170.009025000913914230.0228276760859835
DMSO vs. DISU0.1922301050508460.4320078513377050.881718877024027
DMSO vs. IL70.2141708968548240.2358675931911640.742358431281946
DMSO vs. SAHA-0.04724839637005750.8416078658362930.957490159735697
HIV vs. Mock in Activation0.02275047742605840.9708154558646070.999983755607037
HIV vs. Mock in Latency0.0004421865185729250.9978761607565080.999834320637052
IL7 vs. CD3-0.6158356579518410.05621130109272190.121004418933247
SAHA vs. CD3-0.8949252975640830.01190769007155010.0297494843133721
SAHA vs. IL7-0.2507512562819390.3047372372034620.5535135695391
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change -1.220422172
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.418932 0.00221059
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.043 0.938 0.973 0.966 0.971
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
204117_at 1.38 No upregulated in CD8+ cells
37950_at 1.34 No upregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB01684 1-Hydroxy-1-Thio-Glycerol experimental unknown unknown
DB03382 S-Oxy Cysteine experimental unknown unknown
DB03864 Monothioglycerol experimental unknown unknown
DB07148 (6S)-1-chloro-3-[(4-fluorobenzyl)oxy]-6-(pyrrolidin-1-ylcarbonyl)pyrrolo[1,2-a]pyrazin-4(6H)-one experimental unknown unknown
DB08738 1-{3-oxo-3-[(2S)-2-(pyrrolidin-1-ylcarbonyl)pyrrolidin-1-yl]propyl}-3-phenylquinoxalin-2(1H)-one experimental unknown unknown
DB08739 2-{3-[(2S)-4,4-difluoro-2-(pyrrolidin-1-ylcarbonyl)pyrrolidin-1-yl]-3-oxopropyl}-isoindole-1,3(2H)-dione experimental unknown unknown
DB03535 Z-Pro-Prolinal experimental unknown unknown
DB00107 Oxytocin approved, vet_approved unknown substrate

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3DDU X-ray 1.5Å A=2-710.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04614 Renin-angiotensin system - Homo sapiens (human)