Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002309
UniProt IDP30048
Primary gene name(s)PRDX3
Synonym gene name(s)AOP1
Protein nameThioredoxin-dependent peroxide reductase, mitochondrial
Protein functionInvolved in redox regulation of the cell. Protects radical-sensitive enzymes from oxidative damage by a radical-generating system. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol. {ECO:0000269|PubMed:12492477}.
Subcellular locationMitochondrion.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P30048
Gene Ontology
(Biological Process)
Complete annatation
cell redox homeostasis [GO:0045454];
cellular response to oxidative stress [GO:0034599];
cellular response to reactive oxygen species [GO:0034614];
hydrogen peroxide catabolic process [GO:0042744];
maternal placenta development [GO:0001893];
mitochondrion organization [GO:0007005];
myeloid cell differentiation [GO:0030099];
negative regulation of apoptotic process [GO:0043066];
negative regulation of kinase activity [GO:0033673];
peptidyl-cysteine oxidation [GO:0018171];
positive regulation of cell proliferation [GO:0008284];
positive regulation of NF-kappaB transcription factor activity [GO:0051092];
regulation of mitochondrial membrane potential [GO:0051881];
response to hydrogen peroxide [GO:0042542];
response to lipopolysaccharide [GO:0032496];
response to oxidative stress [GO:0006979];
response to reactive oxygen species [GO:0000302]
Gene Ontology
(Molecular Function)
Complete annatation
alkyl hydroperoxide reductase activity [GO:0008785];
cysteine-type endopeptidase inhibitor activity involved in apoptotic process [GO:0043027];
kinase binding [GO:0019900];
protein C-terminus binding [GO:0008022];
protein kinase binding [GO:0019901];
thioredoxin peroxidase activity [GO:0008379]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
early endosome [GO:0005769];
extracellular exosome [GO:0070062];
mitochondrial matrix [GO:0005759];
mitochondrion [GO:0005739];
myelin sheath [GO:0043209]
Protein-protein interaction116136
Phylogenetic treeP30048
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.366702928885194.85100962669405e-050.000254488051143518
AZA vs. DISU-0.09423813279999210.7096174949088650.972881012378556
AZA vs. IL70.324475534076860.09193777013133550.818689240833796
AZA vs. SAHA-0.5307819263723490.03066783799845420.201872048029999
DISU vs. CD3-1.473377552179088.59406010773611e-050.000496919302125102
DISU vs. IL70.4095107630179110.1050439539879150.426892774636895
DISU vs. SAHA-0.4351492697475520.1387222506558730.478556119891135
DMSO vs. AZA-0.07227889577259570.666637981674811
DMSO vs. CD3-1.450442069028141.085298576875e-056.20258841449998e-05
DMSO vs. DISU0.02005072941765170.9345102253862640.991196107391884
DMSO vs. IL70.4039891074575770.02501896044059460.321809580752529
DMSO vs. SAHA-0.4647977800201820.05024747811488730.253254808864779
HIV vs. Mock in Activation-0.1750750726062770.7781989739658990.999983755607037
HIV vs. Mock in Latency0.01932534434636420.9070885189208870.999834320637052
IL7 vs. CD3-1.034474522976370.001524953939314950.00610183985327696
SAHA vs. CD3-1.92182697081851.49439304264476e-071.49202724558981e-06
SAHA vs. IL7-0.857974901983960.0004766506758177020.00665897338950514
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.109525 0.484724
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
0.144 0.001

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.07 0.974 1.075 1.14 0.959
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
0.6 0.0007 -0.07 0.94 -0.1 0.8843 Cell growth and proliferation at 4 hpi
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
201619_at 1.61 Yes upregulated in CD4+ cells
201619_at 2.74 Yes upregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
5JCG X-ray 2.8Å A/B/C/D/E/F/G/H/I=62-256.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp120 upregulates 17676665
HIV-1 virus replication enhanced by expression of human gene 19460752

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found