Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002305
UniProt IDP50897
Primary gene name(s)PPT1
Synonym gene name(s)CLN1, PPT
Protein namePalmitoyl-protein thioesterase 1
Protein functionRemoves thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons, PubMed:8816748. {ECO:0000269|PubMed:8816748}.
Subcellular locationLysosome {ECO:0000269|PubMed:19941651}. Secreted {ECO:0000250|UniProtKB:P45478}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P50897
Gene Ontology
(Biological Process)
Complete annatation
adult locomotory behavior [GO:0008344];
associative learning [GO:0008306];
brain development [GO:0007420];
cellular protein catabolic process [GO:0044257];
chemical synaptic transmission [GO:0007268];
cofactor metabolic process [GO:0051186];
cofactor transport [GO:0051181];
grooming behavior [GO:0007625];
lipid catabolic process [GO:0016042];
long-chain fatty-acyl-CoA biosynthetic process [GO:0035338];
lysosomal lumen acidification [GO:0007042];
membrane raft organization [GO:0031579];
negative regulation of apoptotic process [GO:0043066];
negative regulation of cell growth [GO:0030308];
negative regulation of neuron apoptotic process [GO:0043524];
nervous system development [GO:0007399];
neuron development [GO:0048666];
neurotransmitter secretion [GO:0007269];
pinocytosis [GO:0006907];
positive regulation of pinocytosis [GO:0048549];
positive regulation of receptor-mediated endocytosis [GO:0048260];
protein catabolic process [GO:0030163];
protein depalmitoylation [GO:0002084];
protein transport [GO:0015031];
receptor-mediated endocytosis [GO:0006898];
regulation of phospholipase A2 activity [GO:0032429];
regulation of synapse structure or activity [GO:0050803];
response to stimulus [GO:0050896];
sphingolipid catabolic process [GO:0030149];
visual perception [GO:0007601]
Gene Ontology
(Molecular Function)
Complete annatation
palmitoyl-(protein hydrolase activity [GO:0008474];
palmitoyl-CoA hydrolase activity [GO:0016290]
Gene Ontology
(Cellular Component)
Complete annatation
axon [GO:0030424];
cytosol [GO:0005829];
dendrite [GO:0030425];
extracellular exosome [GO:0070062];
extracellular region [GO:0005576];
extracellular space [GO:0005615];
Golgi apparatus [GO:0005794];
lysosomal lumen [GO:0043202];
lysosome [GO:0005764];
membrane [GO:0016020];
membrane raft [GO:0045121];
neuronal cell body [GO:0043025];
nucleus [GO:0005634];
synaptic vesicle [GO:0008021]
Protein-protein interaction111530
Phylogenetic treeP50897
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.1389885535093130.6719741391018930.762650167510823
AZA vs. DISU-0.03138628369453720.9012454162202620.993937521279788
AZA vs. IL70.103420893896410.5897104191709840.999311006273513
AZA vs. SAHA-0.6158543082414470.01202259468231160.109768983792347
DISU vs. CD30.09496301211746750.7953187856675330.85958524235695
DISU vs. IL70.1256155680107160.6186968423193810.884732963191489
DISU vs. SAHA-0.5830221197383480.04814403117052430.273287643604886
DMSO vs. AZA-0.04470108697431530.7889305312474791
DMSO vs. CD30.08239711816845920.7969391288108940.856829001965182
DMSO vs. DISU-0.01528789710929830.9500707130142050.993171867725032
DMSO vs. IL70.1553474546415890.3863956443053750.837514642401658
DMSO vs. SAHA-0.5775376665505230.01468464916203160.115326913768097
HIV vs. Mock in Activation0.02165887819424140.972191452195910.999983755607037
HIV vs. Mock in Latency0.03919359259057190.8117544435041530.999834320637052
IL7 vs. CD30.2500579025528250.4366022346758040.57146778869797
SAHA vs. CD3-0.5010567197375540.156422169199590.248894740770545
SAHA vs. IL7-0.7223377094997280.003152611282474680.0275173494337136
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
-0.3002 0.04738

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.292663 0.0253201
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.999 1.073 1.145 1.022 1.141
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
200975_at 1.41 No downregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB02035 1-Hexadecylsulfonyl Fluoride experimental unknown unknown
DB03796 Palmitic Acid experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3GRO X-ray 2.5Å A/B=22-306.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00062 Fatty acid elongation - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa01212 Fatty acid metabolism - Homo sapiens (human)
hsa04142 Lysosome - Homo sapiens (human)