Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002282
UniProt IDQ13356
Primary gene name(s)PPIL2
Synonym gene name(s)unknown
Protein namePeptidyl-prolyl cis-trans isomerase-like 2
Protein functionMay catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides thereby assisting the folding of proteins, By similarity. May also function as a chaperone, playing a role in transport to the cell membrane of BSG for instance, PubMed:15946952. May also have a protein ubiquitin ligase activity acting as an E3 ubiquitin protein ligase or as an ubiquitin-ubiquitin ligase promoting elongation of ubiquitin chains on substrates. By mediating 'Lys-48'-linked polyubiquitination of proteins could target them for proteasomal degradation, PubMed:11435423. {ECO:0000250|UniProtKB:Q08752, ECO:0000269|PubMed:11435423, ECO:0000269|PubMed:15946952, ECO:0000303|PubMed:8660300}.
Subcellular locationNucleus {ECO:0000269|PubMed:11435423, ECO:0000269|PubMed:8660300}. Note=May also localize to the cytoplasm and the cell membrane. {ECO:0000269|PubMed:15946952}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q13356
Gene Ontology
(Biological Process)
Complete annatation
leukocyte migration [GO:0050900];
protein folding [GO:0006457];
protein localization to plasma membrane [GO:0072659];
protein polyubiquitination [GO:0000209]
Gene Ontology
(Molecular Function)
Complete annatation
ligase activity [GO:0016874];
peptidyl-prolyl cis-trans isomerase activity [GO:0003755];
ubiquitin protein ligase activity [GO:0061630];
ubiquitin-ubiquitin ligase activity [GO:0034450]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
Golgi lumen [GO:0005796];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
plasma membrane [GO:0005886]
Protein-protein interaction117260
Phylogenetic treeQ13356
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.6226724607455330.05808889338110550.113016756271781
AZA vs. DISU-0.05973606327787380.8141592705311740.983626273983733
AZA vs. IL7-0.01190286547088780.9506493954633330.999311006273513
AZA vs. SAHA-0.3985159116084960.1035909994449930.408288863693314
DISU vs. CD30.5496408511480210.1330666586088310.233210883835544
DISU vs. IL70.03836517944132520.8796695090209920.976996113795713
DISU vs. SAHA-0.3358334591692350.2557864301950550.634824464852208
DMSO vs. AZA0.1081783462715950.5186926189947091
DMSO vs. CD30.7178483635754730.0254428980873080.0547309233926361
DMSO vs. DISU0.1657927154931180.4988234453713360.907526896115356
DMSO vs. IL7-0.1125769607525420.5313660918965720.89596889695991
DMSO vs. SAHA-0.5112918681781950.03082672935668970.187410100094664
HIV vs. Mock in Activation0.209367987751390.736541143768980.999983755607037
HIV vs. Mock in Latency0.03071020507915080.852423610080710.999834320637052
IL7 vs. CD30.6162248245866440.05582886047629730.120353034033071
SAHA vs. CD30.2010530431656340.5694853455478670.671266652906246
SAHA vs. IL7-0.3889457601880880.1111014544306480.306617232531598
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.0164416 0.951525
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.894 1.118 0.841 0.693 1.057
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1ZKC X-ray 1.6Å A/B=280-457.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04120 Ubiquitin mediated proteolysis - Homo sapiens (human)