Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002257
UniProt IDQ9Y535
Primary gene name(s)POLR3H
Synonym gene name(s)KIAA1665, RPC8
Protein nameDNA-directed RNA polymerase III subunit RPC8
Protein functionDNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs, EBERs induce type I interferon and NF- Kappa-B through the RIG-I pathway, By similarity. {ECO:0000250, ECO:0000269|PubMed:19609254, ECO:0000269|PubMed:19631370}.
Subcellular locationNucleus.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9Y535
Gene Ontology
(Biological Process)
Complete annatation
defense response to virus [GO:0051607];
innate immune response [GO:0045087];
nucleobase-containing compound metabolic process [GO:0006139];
positive regulation of type I interferon production [GO:0032481];
transcription from RNA polymerase III promoter [GO:0006383];
transcription initiation from RNA polymerase III promoter [GO:0006384]
Gene Ontology
(Molecular Function)
Complete annatation
DNA binding [GO:0003677];
DNA-directed RNA polymerase activity [GO:0003899]
Gene Ontology
(Cellular Component)
Complete annatation
centrosome [GO:0005813];
cytosol [GO:0005829];
DNA-directed RNA polymerase III complex [GO:0005666];
intracellular membrane-bounded organelle [GO:0043231];
nucleoplasm [GO:0005654]
Protein-protein interaction128147
Phylogenetic treeQ9Y535
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.9114856361890060.00592471741126810.0167348839622625
AZA vs. DISU-0.2326325848744420.3598594400361030.893791859591363
AZA vs. IL70.2867164848082490.1373459429638760.900378480207128
AZA vs. SAHA-0.5326732481492410.03088171960650750.202885138315811
DISU vs. CD3-1.156145982595290.001628257487798160.00628697576103213
DISU vs. IL70.5099764317095920.04439034409517910.278644082356966
DISU vs. SAHA-0.2978528479113660.3154033104364730.698111806665897
DMSO vs. AZA0.06829186324038670.6852643383289471
DMSO vs. CD3-0.8555605972478030.008349203284081290.0213694779943399
DMSO vs. DISU0.2988280411405610.224147412904790.736728792251948
DMSO vs. IL70.2260718046134780.210196342031480.719751499176064
DMSO vs. SAHA-0.6060638343142590.01082316408909730.0948348290620849
HIV vs. Mock in Activation0.01235386439090570.9841690373379090.999983755607037
HIV vs. Mock in Latency-0.01508733338456840.9276770569840540.999834320637052
IL7 vs. CD3-0.6185228227914730.05641647258723430.12134210777788
SAHA vs. CD3-1.467262065507916.35016462376958e-050.000321346477099421
SAHA vs. IL7-0.8218873395782080.00085627249784670.0103924731340911
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.181408 0.296629
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat activates 1403646

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00230 Purine metabolism - Homo sapiens (human)
hsa00240 Pyrimidine metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa03020 RNA polymerase - Homo sapiens (human)
hsa04623 Cytosolic DNA-sensing pathway - Homo sapiens (human)
hsa05169 Epstein-Barr virus infection - Homo sapiens (human)