Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002253
UniProt IDP19387
Primary gene name(s)POLR2C
Synonym gene name(s)unknown
Protein nameDNA-directed RNA polymerase II subunit RPB3
Protein functionDNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft, By similarity. {ECO:0000250, ECO:0000269|PubMed:9852112}.
Subcellular locationNucleus {ECO:0000269|PubMed:9852112}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P19387
Gene Ontology
(Biological Process)
Complete annatation
7-methylguanosine mRNA capping [GO:0006370];
fibroblast growth factor receptor signaling pathway [GO:0008543];
gene expression [GO:0010467];
gene silencing by RNA [GO:0031047];
mRNA splicing, via spliceosome [GO:0000398];
positive regulation of viral transcription [GO:0050434];
snRNA transcription from RNA polymerase II promoter [GO:0042795];
somatic stem cell population maintenance [GO:0035019];
transcription-coupled nucleotide-excision repair [GO:0006283];
transcription elongation from RNA polymerase II promoter [GO:0006368];
transcription from RNA polymerase II promoter [GO:0006366];
transcription initiation from RNA polymerase II promoter [GO:0006367]
Gene Ontology
(Molecular Function)
Complete annatation
DNA binding [GO:0003677];
DNA-directed RNA polymerase activity [GO:0003899]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
DNA-directed RNA polymerase II, core complex [GO:0005665];
microtubule cytoskeleton [GO:0015630];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction111428
Phylogenetic treeP19387
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.9191370534884370.005341978900309190.0153098128894201
AZA vs. DISU0.2463844595161890.3309143304457350.879082798222567
AZA vs. IL70.01360475414741990.9437045767263240.999311006273513
AZA vs. SAHA-0.4239979888727310.08334796212423180.360306045615334
DISU vs. CD3-0.6852817784267310.05952791396592570.124248653437048
DISU vs. IL7-0.2422141843764030.3372837091781610.71731864783599
DISU vs. SAHA-0.6683304884525690.02242504115755540.174410886165765
DMSO vs. AZA-0.04932518664488990.7691996375529761
DMSO vs. CD3-0.9801272821014370.002376919699801140.00723546075520391
DMSO vs. DISU-0.2976553901364640.2234608622850180.736085960261148
DMSO vs. IL70.07024599021862820.6967209558994210.937788846060647
DMSO vs. SAHA-0.3803498786394780.1078375628728830.389181012944737
HIV vs. Mock in Activation0.05389040776061990.9309435299856350.999983755607037
HIV vs. Mock in Latency0.08145367774430530.6227588986829570.999834320637052
IL7 vs. CD3-0.8989734570323790.005468157537040550.0180590575275662
SAHA vs. CD3-1.366795890074850.0001423776348541410.000653558013174505
SAHA vs. IL7-0.4401522003733220.07174089363381870.233568516336109
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.0841132 0.625981
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.02 1.042 0.905 0.876 1.091
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
5IY6 EM 7.2Å C=1-275.
5IY7 EM 8.6Å C=1-275.
5IY8 EM 7.9Å C=1-275.
5IY9 EM 6.3Å C=1-275.
5IYA EM 5.4Å C=1-275.
5IYB EM 3.9Å C=1-275.
5IYC EM 3.9Å C=1-275.
5IYD EM 3.9Å C=1-275.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00230 Purine metabolism - Homo sapiens (human)
hsa00240 Pyrimidine metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa03020 RNA polymerase - Homo sapiens (human)
hsa05016 Huntington's disease - Homo sapiens (human)
hsa05169 Epstein-Barr virus infection - Homo sapiens (human)
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