Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002235
UniProt IDQ9NVS9
Primary gene name(s)PNPO
Synonym gene name(s)unknown
Protein namePyridoxine-5'-phosphate oxidase
Protein functionCatalyzes the oxidation of either pyridoxine 5'-phosphate, PNP or pyridoxamine 5'-phosphate, PMP into pyridoxal 5'-phosphate, PLP. {ECO:0000269|PubMed:12824491}.
Subcellular locationunknown
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9NVS9
Gene Ontology
(Biological Process)
Complete annatation
pyridoxal phosphate biosynthetic process [GO:0042823];
pyridoxine biosynthetic process [GO:0008615];
vitamin B6 metabolic process [GO:0042816]
Gene Ontology
(Molecular Function)
Complete annatation
FMN binding [GO:0010181];
pyridoxamine-phosphate oxidase activity [GO:0004733]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
extracellular exosome [GO:0070062];
nucleoplasm [GO:0005654]
Protein-protein interaction120463
Phylogenetic treeQ9NVS9
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.268010327470.0001452060054435430.000670715572799597
AZA vs. DISU-0.2405489881170150.3480854038427290.885725755828038
AZA vs. IL70.3159616667051960.1077299681618170.855877773288426
AZA vs. SAHA0.02847111713984830.9083996252979130.980039727734343
DISU vs. CD3-1.520033423773024.22772577703956e-050.000266870763664549
DISU vs. IL70.5469868736744790.03244033012820790.233977977519382
DISU vs. SAHA0.2703898194488080.3579979817238520.734744671289963
DMSO vs. AZA-0.0526654768088990.7605933304002921
DMSO vs. CD3-1.3320274560014.46657625542413e-050.000219396711079115
DMSO vs. DISU0.18606150414350.4516904481786720.888532369154407
DMSO vs. IL70.3757584146605240.04114073543004690.410998400667964
DMSO vs. SAHA0.07487212978983140.754114726957210.928079025112503
HIV vs. Mock in Activation0.06314612819664310.9192109323404320.999983755607037
HIV vs. Mock in Latency-0.07123142616628360.6779414593632690.999834320637052
IL7 vs. CD3-0.9451839894642010.00375808781054210.0132133560924425
SAHA vs. CD3-1.264056690796190.0004372361833111520.00174547000860902
SAHA vs. IL7-0.2903231156952930.2402181085900710.482153879825261
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0242991 0.905684
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.043 1.103 1.239 1.293 1.201
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00114 Pyridoxal Phosphate nutraceutical unknown cofactor
DB03247 Riboflavin Monophosphate experimental unknown unknown
DB03345 Beta-Mercaptoethanol experimental unknown unknown
DB02209 Pyridoxine-5&,39;-Phosphate experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1NRG X-ray 1.9Å A=1-261.
3HY8 X-ray 2.5Å A=1-261.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00750 Vitamin B6 metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)