Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002206
UniProt IDQ8N3E9
Primary gene name(s)PLCD3
Synonym gene name(s)KIAA1964
Protein name1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-3
Protein functionHydrolyzes the phosphatidylinositol 4,5-bisphosphate, PIP2 to generate 2 second messenger molecules diacylglycerol, DAG and inositol 1,4,5-trisphosphate, IP3. DAG mediates the activation of protein kinase C, PKC, while IP3 releases Ca(2+ from intracellular stores. Essential for trophoblast and placental development. May participate in cytokinesis by hydrolyzing PIP2 at the cleavage furrow.
Subcellular locationMembrane;
Peripheral membrane protein. Cytoplasm. Cleavage furrow. Note=Localizes at the cleavage furrow during cytokinesis.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q8N3E9
Gene Ontology
(Biological Process)
Complete annatation
angiogenesis [GO:0001525];
inositol phosphate metabolic process [GO:0043647];
intracellular signal transduction [GO:0035556];
labyrinthine layer blood vessel development [GO:0060716];
lipid catabolic process [GO:0016042];
regulation of cell proliferation [GO:0042127]
Gene Ontology
(Molecular Function)
Complete annatation
metal ion binding [GO:0046872];
phosphatidylinositol phospholipase C activity [GO:0004435];
signal transducer activity [GO:0004871]
Gene Ontology
(Cellular Component)
Complete annatation
cleavage furrow [GO:0032154];
cytosol [GO:0005829];
plasma membrane [GO:0005886]
Protein-protein interaction125222
Phylogenetic treeQ8N3E9
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.1606657848061070.6391909011707390.736986326686458
AZA vs. DISU0.7474951864651710.005789605447914180.198798433990566
AZA vs. IL7-0.3333105984274450.1373894051931530.900378480207128
AZA vs. SAHA0.881950380096270.0008031309059195470.0178297426742728
DISU vs. CD30.5723756243607660.1276100143459230.225800370495415
DISU vs. IL7-1.089233152823076.00151568054264e-050.00350909075906982
DISU vs. SAHA0.137229789875120.6489711918107440.891575615632762
DMSO vs. AZA0.0255906741606250.8985023232588261
DMSO vs. CD3-0.1501253192166970.6527141151777320.741452129780767
DMSO vs. DISU-0.7244960091235360.005453722832378440.158663326416878
DMSO vs. IL7-0.3516777081270680.09509756672040510.566191081481085
DMSO vs. SAHA0.8516228193752460.0007692047290616120.014958302544113
HIV vs. Mock in Activation0.462242250745180.4751093852969990.999983755607037
HIV vs. Mock in Latency-0.06476089155394560.8203179160167840.999834320637052
IL7 vs. CD3-0.4898313781909410.1482002306366950.256761158205391
SAHA vs. CD30.696865044093240.05666689047263720.109891910876675
SAHA vs. IL71.212476679015334.56666289072594e-060.00016389970574953
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.374812 0.113578
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat activates 7589147
Envelope surface glycoprotein gp120 inactivates 1979339

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00562 Inositol phosphate metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa04020 Calcium signaling pathway - Homo sapiens (human)
hsa04070 Phosphatidylinositol signaling system - Homo sapiens (human)
hsa04919 Thyroid hormone signaling pathway - Homo sapiens (human)
hsa04933 AGE-RAGE signaling pathway in diabetic complications - Homo sapiens (human)