Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002201
UniProt IDO60733
Primary gene name(s)PLA2G6
Synonym gene name(s)PLPLA9
Protein name85/88 kDa calcium-independent phospholipase A2
Protein functionCatalyzes the release of fatty acids from phospholipids. It has been implicated in normal phospholipid remodeling, nitric oxide-induced or vasopressin-induced arachidonic acid release and in leukotriene and prostaglandin production. May participate in fas mediated apoptosis and in regulating transmembrane ion flux in glucose-stimulated B-cells. Has a role in cardiolipin, CL deacylation. Required for both speed and directionality of monocyte MCP1/CCL2-induced chemotaxis through regulation of F-actin polymerization at the pseudopods.; FUNCTION: Isoform ankyrin-iPLA2-1 and isoform ankyrin-iPLA2-2, which lack the catalytic domain, are probably involved in the negative regulation of iPLA2 activity.
Subcellular locationIsoform LH-iPLA2: Membrane;
Peripheral membrane protein. Note=Recruited to the membrane-enriched pseudopod upon MCP1/CCL2 stimulation in monocytes.;
SUBCELLULAR LOCATION: Isoform SH-iPLA2: Cytoplasm.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O60733
Gene Ontology
(Biological Process)
Complete annatation
antibacterial humoral response [GO:0019731];
cardiolipin biosynthetic process [GO:0032049];
chemotaxis [GO:0006935];
Fc-gamma receptor signaling pathway involved in phagocytosis [GO:0038096];
lipid catabolic process [GO:0016042];
maternal process involved in female pregnancy [GO:0060135];
memory [GO:0007613];
negative regulation of synaptic transmission, glutamatergic [GO:0051967];
phosphatidylcholine acyl-chain remodeling [GO:0036151];
phosphatidylethanolamine acyl-chain remodeling [GO:0036152];
positive regulation of arachidonic acid secretion [GO:0090238];
positive regulation of ceramide biosynthetic process [GO:2000304];
positive regulation of cytosolic calcium ion concentration [GO:0007204];
positive regulation of exocytosis [GO:0045921];
positive regulation of insulin secretion involved in cellular response to glucose stimulus [GO:0035774];
positive regulation of protein kinase C signaling [GO:0090037];
positive regulation of protein phosphorylation [GO:0001934];
positive regulation of release of cytochrome c from mitochondria [GO:0090200];
positive regulation of vasodilation [GO:0045909];
regulation of store-operated calcium channel activity [GO:1901339];
response to endoplasmic reticulum stress [GO:0034976];
urinary bladder smooth muscle contraction [GO:0014832]
Gene Ontology
(Molecular Function)
Complete annatation
calcium-independent phospholipase A2 activity [GO:0047499];
hydrolase activity [GO:0016787];
phospholipase A2 activity [GO:0004623]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
extracellular space [GO:0005615];
membrane [GO:0016020];
microtubule organizing center [GO:0005815];
mitochondrion [GO:0005739]
Protein-protein interaction113986
Phylogenetic treeO60733
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.253629300308320.0001752910971852820.00079006800740926
AZA vs. DISU0.1874221261452420.4654353339898260.925522269888509
AZA vs. IL7-0.4494729191450790.06488641198617540.730420379288739
AZA vs. SAHA-0.1997070356068640.5846710984863250.862266091608479
DISU vs. CD31.427154669719610.0001287042339636060.000707544720192339
DISU vs. IL7-0.6466550970773770.01202189218598190.131041232612927
DISU vs. SAHA-0.3841659561558220.3079400831218960.691958400585968
DMSO vs. AZA0.1208028292173110.5403770157010711
DMSO vs. CD31.36034159386973.19686723626589e-050.000162595560142963
DMSO vs. DISU-0.06929700258649390.779929986339080.971585548411322
DMSO vs. IL7-0.5625603335013980.02085623880575660.297102672049699
DMSO vs. SAHA-0.3249071335115680.370639937792350.718168488360569
HIV vs. Mock in Activation0.2546015391919090.6847270172655190.999983755607037
HIV vs. Mock in Latency0.2394945084085740.2609245007163460.999834320637052
IL7 vs. CD30.8092671735149220.01293283488196530.036752756443413
SAHA vs. CD31.031148824394670.004445322427179650.0129214629242249
SAHA vs. IL70.2466177915425140.5287654951336520.749751770637256
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0356257 0.898621
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB01103 Quinacrine approved yes inhibitor

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp120 activates 9377118
97459299745929

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00564 Glycerophospholipid metabolism - Homo sapiens (human)
hsa00565 Ether lipid metabolism - Homo sapiens (human)
hsa00590 Arachidonic acid metabolism - Homo sapiens (human)
hsa00591 Linoleic acid metabolism - Homo sapiens (human)
hsa00592 alpha-Linolenic acid metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa04014 Ras signaling pathway - Homo sapiens (human)
hsa04270 Vascular smooth muscle contraction - Homo sapiens (human)
hsa04666 Fc gamma R-mediated phagocytosis - Homo sapiens (human)
hsa04750 Inflammatory mediator regulation of TRP channels - Homo sapiens (human)
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