Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002200
UniProt IDQ8NCC3
Primary gene name(s)PLA2G15
Synonym gene name(s)LYPLA3
Protein nameGroup XV phospholipase A2
Protein functionHas transacylase and calcium-independent phospholipase A2 activity, PubMed:20410020, PubMed:23958596. Catalyzes the formation of 1-O-acyl-N-acetylsphingosine and the concomitant release of a lyso-phospholipid, PubMed:11790796, PubMed:25727495. Has high activity with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, POPC and 1,2-dioleoyl-sn-glycero-3-phosphocholine, DOPC, catalyzing the transfer of oleic acid to N-acetyl-sphingosine. Required for normal phospholipid degradation in alveolar and peritoneal macrophages and in spleen, By similarity. May have weak lysophospholipase activity, PubMed:10092508. {ECO:0000250|UniProtKB:Q8VEB4, ECO:0000269|PubMed:10092508, ECO:0000269|PubMed:11790796, ECO:0000269|PubMed:20410020, ECO:0000269|PubMed:23958596, ECO:0000269|PubMed:25727495}.
Subcellular locationLysosome {ECO:0000269|PubMed:23958596}. Secreted {ECO:0000269|PubMed:10092508, ECO:0000269|PubMed:20410020}. Membrane {ECO:0000269|PubMed:25727495};
Peripheral membrane protein {ECO:0000269|PubMed:25727495}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q8NCC3
Gene Ontology
(Biological Process)
Complete annatation
ceramide metabolic process [GO:0006672];
fatty acid catabolic process [GO:0009062];
glycerophospholipid metabolic process [GO:0006650];
phosphatidylcholine catabolic process [GO:0034638];
phosphatidylethanolamine catabolic process [GO:0046338]
Gene Ontology
(Molecular Function)
Complete annatation
calcium-independent phospholipase A2 activity [GO:0047499];
lysophospholipase activity [GO:0004622];
O-acyltransferase activity [GO:0008374];
phospholipid binding [GO:0005543]
Gene Ontology
(Cellular Component)
Complete annatation
extracellular exosome [GO:0070062];
extracellular space [GO:0005615];
lysosome [GO:0005764];
membrane [GO:0016020];
mitochondrion [GO:0005739]
Protein-protein interaction117181
Phylogenetic treeQ8NCC3
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.1953853534746260.5541538274956070.665241967672756
AZA vs. DISU0.04174870120822620.870761372309530.991631204917382
AZA vs. IL70.1400027668276660.4780441486721270.999311006273513
AZA vs. SAHA0.1733890504177010.4858586806030210.811914714447307
DISU vs. CD30.2254492100228010.5366862148386230.656976449770781
DISU vs. IL70.08787521185409490.7306401686688290.932850179574094
DISU vs. SAHA0.1344663997916270.6479642160314320.891335222691649
DMSO vs. AZA-0.0569073520137650.7436791164041071
DMSO vs. CD30.1259421499850760.6960512668573530.77691430208239
DMSO vs. DISU-0.1008851743985090.6835843232983310.957324272823274
DMSO vs. IL70.2044420949394550.2687452601435150.767539423873874
DMSO vs. SAHA0.2262494569619070.3465583283186940.697792994801741
HIV vs. Mock in Activation0.05539754129925560.9296652319900470.999983755607037
HIV vs. Mock in Latency0.05546640698193210.7476540055735220.999834320637052
IL7 vs. CD30.3397946048780190.2942577391117180.432167280355418
SAHA vs. CD30.3458426713272030.3336713248047620.448886526049613
SAHA vs. IL70.03224490971871130.8966468347662340.960143187185462
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.173859 0.292556
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4X90 X-ray 1.8Å A/B/C/D=34-412.
4X91 X-ray 2.3Å A/B/C/D=34-412.
4X92 X-ray 3.0Å A=34-412.
4X93 X-ray 2.6Å A/B=34-412.
4X94 X-ray 2.7Å A=34-412.
4X95 X-ray 3.0Å A/B=34-412.
4X97 X-ray 2.6Å A/B/C/D=34-412.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00564 Glycerophospholipid metabolism - Homo sapiens (human)
hsa04142 Lysosome - Homo sapiens (human)