Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002187
UniProt IDP48426
Primary gene name(s)PIP4K2A
Synonym gene name(s)PIP5K2, PIP5K2A
Protein namePhosphatidylinositol 5-phosphate 4-kinase type-2 alpha
Protein functionCatalyzes the phosphorylation of phosphatidylinositol 5-phosphate, PtdIns5P on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate, PtdIns(4,5P2. May exert its function by regulating the levels of PtdIns5P, which functions in the cytosol by increasing AKT activity and in the nucleus signals through ING2. May regulate the pool of cytosolic PtdIns5P in response to the activation of tyrosine phosphorylation. May negatively regulate insulin-stimulated glucose uptake by lowering the levels of PtdIns5P. May be involved in thrombopoiesis, and the terminal maturation of megakaryocytes and regulation of their size. {ECO:0000269|PubMed:18364242}.
Subcellular locationCell membrane {ECO:0000250}. Nucleus {ECO:0000269|PubMed:20583997}. Cytoplasm {ECO:0000269|PubMed:20583997}. Note=May translocate from the cytosol to the cell membrane upon activation of tyrosine phosphorylation. May translocate from the inner to the outer segments of the rod photoreceptor cells in response to light, By similarity. Localization to the nucleus is modulated by the interaction with PIP4K2B. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P48426
Gene Ontology
(Biological Process)
Complete annatation
megakaryocyte development [GO:0035855];
phosphatidylinositol biosynthetic process [GO:0006661];
positive regulation of autophagosome assembly [GO:2000786];
regulation of autophagy [GO:0010506];
regulation of phosphatidylinositol 3-kinase signaling [GO:0014066]
Gene Ontology
(Molecular Function)
Complete annatation
1-phosphatidylinositol-3-phosphate 4-kinase activity [GO:0052811];
1-phosphatidylinositol-4-phosphate 5-kinase activity [GO:0016308];
1-phosphatidylinositol-5-phosphate 4-kinase activity [GO:0016309];
ATP binding [GO:0005524]
Gene Ontology
(Cellular Component)
Complete annatation
autophagosome [GO:0005776];
cytosol [GO:0005829];
nucleus [GO:0005634];
plasma membrane [GO:0005886]
Protein-protein interaction111322
Phylogenetic treeP48426
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.093266791859880.001034922479585250.00375411591150637
AZA vs. DISU-0.1360206114384560.5905203038475660.955158276076142
AZA vs. IL70.0404967076149040.8328405336366340.999311006273513
AZA vs. SAHA-0.1963124596916870.420325749006680.77445379597836
DISU vs. CD30.9458328915484930.009789612746563090.0284332606053357
DISU vs. IL70.1671146909095540.5065181011112580.830244685685791
DISU vs. SAHA-0.05909370194560380.8392235435649260.958177123111137
DMSO vs. AZA-0.04918464424786820.7685041559590471
DMSO vs. CD31.0340518586710.001446919671370120.00471814823575559
DMSO vs. DISU0.08540891871420640.7260024766632130.964942874095702
DMSO vs. IL70.09668774886611830.5899767402700830.909845858619163
DMSO vs. SAHA-0.1540432375310150.5128897847774360.820505404616239
HIV vs. Mock in Activation0.2108265752305120.7350041089227760.999983755607037
HIV vs. Mock in Latency0.02715142772056660.8690247684001330.999834320637052
IL7 vs. CD31.140892883398590.0004707789470604910.00222586294512007
SAHA vs. CD30.8726133760395140.0148979136956850.035939100271166
SAHA vs. IL7-0.2400063017478360.3239947885980020.573766147007317
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.533341 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.949 0.96 1.02 1.096 1.041
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2YBX X-ray 2.5Å A/B=35-405.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00562 Inositol phosphate metabolism - Homo sapiens (human)
hsa04070 Phosphatidylinositol signaling system - Homo sapiens (human)
hsa04810 Regulation of actin cytoskeleton - Homo sapiens (human)