Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002181
UniProt IDQ6ZUJ8
Primary gene name(s)PIK3AP1
Synonym gene name(s)BCAP
Protein namePhosphoinositide 3-kinase adapter protein 1
Protein functionSignaling adapter that contributes to B-cell development by linking B-cell receptor, BCR signaling to the phosphoinositide 3-kinase, PI3K-Akt signaling pathway. Has a complementary role to the BCR coreceptor CD19, coupling BCR and PI3K activation by providing a docking site for the PI3K subunit PIK3R1. Alternatively, links Toll-like receptor, TLR signaling to PI3K activation, a process preventing excessive inflammatory cytokine production. Also involved in the activation of PI3K in natural killer cells. May be involved in the survival of mature B-cells via activation of REL. {ECO:0000269|PubMed:15893754}.
Subcellular locationCytoplasm {ECO:0000250}. Cell membrane {ECO:0000250};
Peripheral membrane protein {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q6ZUJ8
Gene Ontology
(Biological Process)
Complete annatation
positive regulation of phosphatidylinositol 3-kinase signaling [GO:0014068];
regulation of inflammatory response [GO:0050727];
toll-like receptor 2 signaling pathway [GO:0034134];
toll-like receptor 4 signaling pathway [GO:0034142];
toll-like receptor 7 signaling pathway [GO:0034154];
toll-like receptor 9 signaling pathway [GO:0034162]
Gene Ontology
(Molecular Function)
Complete annatation
phosphatidylinositol 3-kinase regulatory subunit binding [GO:0036312]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
membrane [GO:0016020];
plasma membrane [GO:0005886]
Protein-protein interaction125621
Phylogenetic treeQ6ZUJ8
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.8321176204006930.01487785032448650.0365269522871251
AZA vs. DISU0.02584444300399330.9377446400290240.995602387927702
AZA vs. IL7-0.5742566836062260.08599049090170390.800246832419973
AZA vs. SAHA-0.425287400861660.3019658482507290.673946283170194
DISU vs. CD3-0.8210738734559370.02936613904084440.0703312964572114
DISU vs. IL7-0.6063129237201830.066603451515870.342489022142181
DISU vs. SAHA-0.4528162578253530.2655704705749960.643827393498775
DMSO vs. AZA-0.01095941429303060.9751525200315311
DMSO vs. CD3-0.8641236761256420.009549370712836240.0239529253727735
DMSO vs. DISU-0.04075832086745490.9054435148522510.988283279918411
DMSO vs. IL7-0.554504493912620.1120130354462690.600069832747868
DMSO vs. SAHA-0.4209828141717880.3177143860248520.670251051481039
HIV vs. Mock in Activation0.09414734479616480.8821741245653630.999983755607037
HIV vs. Mock in Latency-0.4337842476001890.1093793636664570.999834320637052
IL7 vs. CD3-1.396980185838524.30395716509713e-050.000278063181664863
SAHA vs. CD3-1.284218729018470.0006635215972158330.0025104871880599
SAHA vs. IL70.1394028671155240.7326942612223460.87823101889835
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock 0.79 1.82E-10 2.61E-08
Infected vs. Bystander 0.833 6.37E-11 6.16E-09
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 1.07464 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04151 PI3K-Akt signaling pathway - Homo sapiens (human)
hsa04662 B cell receptor signaling pathway - Homo sapiens (human)
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