Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002173
UniProt IDQ9NRD5
Primary gene name(s)PICK1
Synonym gene name(s)PRKCABP
Protein namePRKCA-binding protein
Protein functionProbable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate ASIC1/ASIC3 channel. Regulates actin polymerization by inhibiting the actin-nucleating activity of the Arp2/3 complex; the function is competetive with nucleation promoting factors and is linked to neuronal morphology regulation and AMPA receptor, AMPAR endocytosis. Via interaction with the Arp2/3 complex involved in regulation of synaptic plasicity of excitatory synapses and required for spine shrinkage during long-term depression, LTD. Involved in regulation of astrocyte morphology, antagonistic to Arp2/3 complex activator WASL/N-WASP function. {ECO:0000269|PubMed:20403402}.
Subcellular locationCytoplasm, perinuclear region. Membrane {ECO:0000250};
Peripheral membrane protein {ECO:0000250}. Membrane {ECO:0000250};
Lipid-anchor {ECO:0000250}. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density {ECO:0000250}. Cell junction, synapse, synaptosome {ECO:0000250}. Cytoplasm, cytoskeleton {ECO:0000250}. Note=Also membrane-associated, present at excitatory synapses. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9NRD5
Gene Ontology
(Biological Process)
Complete annatation
cellular response to decreased oxygen levels [GO:0036294];
cellular response to glucose starvation [GO:0042149];
dendritic spine maintenance [GO:0097062];
dendritic spine organization [GO:0097061];
DNA methylation involved in embryo development [GO:0043045];
DNA methylation involved in gamete generation [GO:0043046];
glial cell development [GO:0021782];
long term synaptic depression [GO:0060292];
monoamine transport [GO:0015844];
negative regulation of Arp2/3 complex-mediated actin nucleation [GO:0034316];
neuronal ion channel clustering [GO:0045161];
positive regulation of receptor internalization [GO:0002092];
protein kinase C-activating G-protein coupled receptor signaling pathway [GO:0007205];
protein phosphorylation [GO:0006468];
receptor clustering [GO:0043113];
regulation of Arp2/3 complex-mediated actin nucleation [GO:0034315];
retrograde vesicle-mediated transport, Golgi to ER [GO:0006890]
Gene Ontology
(Molecular Function)
Complete annatation
actin filament binding [GO:0051015];
Arp2/3 complex binding [GO:0071933];
ATPase activity [GO:0016887];
enzyme binding [GO:0019899];
G-protein coupled receptor binding [GO:0001664];
identical protein binding [GO:0042802];
metal ion binding [GO:0046872];
protein C-terminus binding [GO:0008022];
protein domain specific binding [GO:0019904];
protein kinase C binding [GO:0005080];
receptor binding [GO:0005102]
Gene Ontology
(Cellular Component)
Complete annatation
cell junction [GO:0030054];
cytoplasm [GO:0005737];
cytoskeleton [GO:0005856];
endocytic vesicle membrane [GO:0030666];
Golgi apparatus [GO:0005794];
neuron projection [GO:0043005];
perinuclear region of cytoplasm [GO:0048471];
plasma membrane [GO:0005886];
postsynaptic density [GO:0014069];
postsynaptic membrane [GO:0045211];
presynaptic membrane [GO:0042734];
synapse [GO:0045202]
Protein-protein interaction114849
Phylogenetic treeQ9NRD5
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.02820139417080730.9319927019977080.956293417149531
AZA vs. DISU-0.1659341102713750.5560459647571070.948689448488927
AZA vs. IL7-0.1291587643148610.5584579530980360.999311006273513
AZA vs. SAHA-1.456185706777660.04805168830651290.263969077817921
DISU vs. CD3-0.1497244871281520.6818212781743060.775915291197304
DISU vs. IL70.02587631328179930.9281462924338280.986449898367171
DISU vs. SAHA-1.286495044782680.09946945647966780.403853496391453
DMSO vs. AZA0.03585995722586480.8560665193619661
DMSO vs. CD30.05494335017886150.8646996725219870.907341406041912
DMSO vs. DISU0.2001001715985580.461492081543470.892965804867364
DMSO vs. IL7-0.157869913778970.4476510561743090.868701134765033
DMSO vs. SAHA-1.497041867841910.04158047520692780.227462556023088
HIV vs. Mock in Activation0.215248653907160.732167304604060.999983755607037
HIV vs. Mock in Latency0.1534326379277530.3697097548068940.999834320637052
IL7 vs. CD3-0.09725786625445740.7645903471999290.843741877395456
SAHA vs. CD3-1.449883470888370.04844012526468330.0964068525803985
SAHA vs. IL7-1.327718910421660.06751793874744650.224226791953956
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.15183 0.458239
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2GZV X-ray 1.1Å A=19-105.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found