Database ID | HIV0002173 |
UniProt ID | Q9NRD5 |
Primary gene name(s) | PICK1 |
Synonym gene name(s) | PRKCABP |
Protein name | PRKCA-binding protein |
Protein function | Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate ASIC1/ASIC3 channel. Regulates actin polymerization by inhibiting the actin-nucleating activity of the Arp2/3 complex; the function is competetive with nucleation promoting factors and is linked to neuronal morphology regulation and AMPA receptor, AMPAR endocytosis. Via interaction with the Arp2/3 complex involved in regulation of synaptic plasicity of excitatory synapses and required for spine shrinkage during long-term depression, LTD. Involved in regulation of astrocyte morphology, antagonistic to Arp2/3 complex activator WASL/N-WASP function. {ECO:0000269|PubMed:20403402}. |
Subcellular location | Cytoplasm, perinuclear region. Membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Membrane {ECO:0000250}; Lipid-anchor {ECO:0000250}. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density {ECO:0000250}. Cell junction, synapse, synaptosome {ECO:0000250}. Cytoplasm, cytoskeleton {ECO:0000250}. Note=Also membrane-associated, present at excitatory synapses. {ECO:0000250}. |
ECO code | Click here for more information. |
Amino acid sequence FASTA format: Q9NRD5 |
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Gene Ontology (Biological Process) Complete annatation | cellular response to decreased oxygen levels [GO:0036294]; cellular response to glucose starvation [GO:0042149]; dendritic spine maintenance [GO:0097062]; dendritic spine organization [GO:0097061]; DNA methylation involved in embryo development [GO:0043045]; DNA methylation involved in gamete generation [GO:0043046]; glial cell development [GO:0021782]; long term synaptic depression [GO:0060292]; monoamine transport [GO:0015844]; negative regulation of Arp2/3 complex-mediated actin nucleation [GO:0034316]; neuronal ion channel clustering [GO:0045161]; positive regulation of receptor internalization [GO:0002092]; protein kinase C-activating G-protein coupled receptor signaling pathway [GO:0007205]; protein phosphorylation [GO:0006468]; receptor clustering [GO:0043113]; regulation of Arp2/3 complex-mediated actin nucleation [GO:0034315]; retrograde vesicle-mediated transport, Golgi to ER [GO:0006890] |
Gene Ontology (Molecular Function) Complete annatation | actin filament binding [GO:0051015]; Arp2/3 complex binding [GO:0071933]; ATPase activity [GO:0016887]; enzyme binding [GO:0019899]; G-protein coupled receptor binding [GO:0001664]; identical protein binding [GO:0042802]; metal ion binding [GO:0046872]; protein C-terminus binding [GO:0008022]; protein domain specific binding [GO:0019904]; protein kinase C binding [GO:0005080]; receptor binding [GO:0005102] |
Gene Ontology (Cellular Component) Complete annatation | cell junction [GO:0030054]; cytoplasm [GO:0005737]; cytoskeleton [GO:0005856]; endocytic vesicle membrane [GO:0030666]; Golgi apparatus [GO:0005794]; neuron projection [GO:0043005]; perinuclear region of cytoplasm [GO:0048471]; plasma membrane [GO:0005886]; postsynaptic density [GO:0014069]; postsynaptic membrane [GO:0045211]; presynaptic membrane [GO:0042734]; synapse [GO:0045202] |
Protein-protein interaction | 114849 |
Phylogenetic tree | Q9NRD5 |
HIV replication factor status |
Zhou et al., Cell. Host. Microbe., 2008 unknown Brass et al., Science, 2008 unknown Smith et al., J. Immunol, 2010 unknown |
Interferon-stimulated gene status |
Lu et al., J. Virol., 2011 Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown; Schoggins JW and Rice CM, Curr. Opin. Virol., 2011 Targeted viruses: unknown Viral life cycle: unknown Mechanism related to antiviral activity: unknown |
Anti-viral restriction factor |
Liu et al., Retrovirology, 2011 unknown (Triplicates) |
Up-regulated;
Down-regulated
For brief introduction to each study, please go to the help page.
(1). Mohammadi et al., PLoS Pathog., 2014 Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model DMSO: Dimethyl suloxyde (negative control) - 0.0033% final SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies IL7: Interleukin-7 based stimulation DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM |
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Experimental Condition | Log2 Fold Change | P value | Adjusted P value |
AZA vs. CD3 | -0.0282013941708073 | 0.931992701997708 | 0.956293417149531 |
AZA vs. DISU | -0.165934110271375 | 0.556045964757107 | 0.948689448488927 |
AZA vs. IL7 | -0.129158764314861 | 0.558457953098036 | 0.999311006273513 |
AZA vs. SAHA | -1.45618570677766 | 0.0480516883065129 | 0.263969077817921 |
DISU vs. CD3 | -0.149724487128152 | 0.681821278174306 | 0.775915291197304 |
DISU vs. IL7 | 0.0258763132817993 | 0.928146292433828 | 0.986449898367171 |
DISU vs. SAHA | -1.28649504478268 | 0.0994694564796678 | 0.403853496391453 |
DMSO vs. AZA | 0.0358599572258648 | 0.856066519361966 | 1 |
DMSO vs. CD3 | 0.0549433501788615 | 0.864699672521987 | 0.907341406041912 |
DMSO vs. DISU | 0.200100171598558 | 0.46149208154347 | 0.892965804867364 |
DMSO vs. IL7 | -0.15786991377897 | 0.447651056174309 | 0.868701134765033 |
DMSO vs. SAHA | -1.49704186784191 | 0.0415804752069278 | 0.227462556023088 |
HIV vs. Mock in Activation | 0.21524865390716 | 0.73216730460406 | 0.999983755607037 |
HIV vs. Mock in Latency | 0.153432637927753 | 0.369709754806894 | 0.999834320637052 |
IL7 vs. CD3 | -0.0972578662544574 | 0.764590347199929 | 0.843741877395456 |
SAHA vs. CD3 | -1.44988347088837 | 0.0484401252646833 | 0.0964068525803985 |
SAHA vs. IL7 | -1.32771891042166 | 0.0675179387474465 | 0.224226791953956 |
(2). Iglesias-Ussel et al., J. Virol., 2013 Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model |
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Log2 Fold Change | P Value | ||
unknown | unknown |
(1). Imbeault et al., PloS Pathog., 2012 Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells |
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Experiment type | Log2 Fold Change | P Value | Adjusted P Value | ||
Infected vs. Mock | unknown | unknown | unknown | ||
Infected vs. Bystander | unknown | unknown | unknown | ||
(2). Lefebvre et al., J. Virol., 2011 Transcriptome analysis of T-cell line (Sup T1) |
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Log2 Fold Change | unknown | ||||
(3). Li et al., J. Immunol., 2013 Lymphatic tissue |
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Acute Fold Change | Acute P Value | Asymt Fold Change | Asypt P Value | AIDS Fold Change | AIDS P Value |
unknown | unknown | unknown | unknown | unknown | unknown |
(4). Chang et al., MBio., 2011 Transcriptome analysis of T-cell line (Sup T1) Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation |
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Up-regulated (True) | TRUE | ||||
(5). Sherrill-Mix et al., BMC Retrovirol., 2015 Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based |
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Test Status | Log2 Fold Change | P Value | |||
OK | 0.15183 | 0.458239 | |||
(6). Rotger et al., PLoS Pathog., 2010 Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient (Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach) |
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Log2 Fold Change | P Value | ||||
unknown | unknown |
(1). Greenwood et al., Elife, 2016 Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset |
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6 h | 24 h | 48 h | 72 h | RTi | ||
unknown | unknown | unknown | unknown | unknown | ||
(2). Navare et al., Virology, 2012 SUP-T1 cell line |
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FC-4hpi | P-value | FC-8hpi | P-value | FC-20hpi | P-value | Category |
unknown | unknown | unknown | unknown | unknown | unknown | unknown |
(3). Hyrcza et al., J. Virolo., 2007 Primary human CD4+ and CD8+ T Cells |
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Affymetrix Prob ID | Fold Change | In CD8? | Category | |||
unknown | unknown | unknown | unknown |
PDB Accession | Method | Resolution | Chain | Structure Preview |
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2GZV | X-ray | 1.1Å | A=19-105. | ![]() |
Pathway Accession Number | Description | not found |
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