Protein Information (annotations from UniProt)
| Database ID | HIV0002173 |
| UniProt ID | Q9NRD5 |
| Primary gene name(s) | PICK1 |
| Synonym gene name(s) | PRKCABP |
| Protein name | PRKCA-binding protein |
| Protein function | Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate ASIC1/ASIC3 channel. Regulates actin polymerization by inhibiting the actin-nucleating activity of the Arp2/3 complex; the function is competetive with nucleation promoting factors and is linked to neuronal morphology regulation and AMPA receptor, AMPAR endocytosis. Via interaction with the Arp2/3 complex involved in regulation of synaptic plasicity of excitatory synapses and required for spine shrinkage during long-term depression, LTD. Involved in regulation of astrocyte morphology, antagonistic to Arp2/3 complex activator WASL/N-WASP function. {ECO:0000269|PubMed:20403402}. |
| Subcellular location | Cytoplasm, perinuclear region. Membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Membrane {ECO:0000250}; Lipid-anchor {ECO:0000250}. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density {ECO:0000250}. Cell junction, synapse, synaptosome {ECO:0000250}. Cytoplasm, cytoskeleton {ECO:0000250}. Note=Also membrane-associated, present at excitatory synapses. {ECO:0000250}. |
| ECO code | Click here for more information. |
| Amino acid sequence FASTA format: Q9NRD5 |
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| Gene Ontology (Biological Process) Complete annatation | cellular response to decreased oxygen levels [GO:0036294]; cellular response to glucose starvation [GO:0042149]; dendritic spine maintenance [GO:0097062]; dendritic spine organization [GO:0097061]; DNA methylation involved in embryo development [GO:0043045]; DNA methylation involved in gamete generation [GO:0043046]; glial cell development [GO:0021782]; long term synaptic depression [GO:0060292]; monoamine transport [GO:0015844]; negative regulation of Arp2/3 complex-mediated actin nucleation [GO:0034316]; neuronal ion channel clustering [GO:0045161]; positive regulation of receptor internalization [GO:0002092]; protein kinase C-activating G-protein coupled receptor signaling pathway [GO:0007205]; protein phosphorylation [GO:0006468]; receptor clustering [GO:0043113]; regulation of Arp2/3 complex-mediated actin nucleation [GO:0034315]; retrograde vesicle-mediated transport, Golgi to ER [GO:0006890] |
| Gene Ontology (Molecular Function) Complete annatation | actin filament binding [GO:0051015]; Arp2/3 complex binding [GO:0071933]; ATPase activity [GO:0016887]; enzyme binding [GO:0019899]; G-protein coupled receptor binding [GO:0001664]; identical protein binding [GO:0042802]; metal ion binding [GO:0046872]; protein C-terminus binding [GO:0008022]; protein domain specific binding [GO:0019904]; protein kinase C binding [GO:0005080]; receptor binding [GO:0005102] |
| Gene Ontology (Cellular Component) Complete annatation | cell junction [GO:0030054]; cytoplasm [GO:0005737]; cytoskeleton [GO:0005856]; endocytic vesicle membrane [GO:0030666]; Golgi apparatus [GO:0005794]; neuron projection [GO:0043005]; perinuclear region of cytoplasm [GO:0048471]; plasma membrane [GO:0005886]; postsynaptic density [GO:0014069]; postsynaptic membrane [GO:0045211]; presynaptic membrane [GO:0042734]; synapse [GO:0045202] |
| Protein-protein interaction | 114849 |
| Phylogenetic tree | Q9NRD5 |
| HIV replication factor status |
Zhou et al., Cell. Host. Microbe., 2008 unknown Brass et al., Science, 2008 unknown Smith et al., J. Immunol, 2010 unknown |
| Interferon-stimulated gene status |
Lu et al., J. Virol., 2011 Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown; Schoggins JW and Rice CM, Curr. Opin. Virol., 2011 Targeted viruses: unknown Viral life cycle: unknown Mechanism related to antiviral activity: unknown |
| Anti-viral restriction factor |
Liu et al., Retrovirology, 2011 unknown (Triplicates) |
Gene Expression Profile top
Up-regulated; 
Down-regulated
For brief introduction to each study, please go to the help page.
Gene expression during HIV latency
| (1). Mohammadi et al., PLoS Pathog., 2014 Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model DMSO: Dimethyl suloxyde (negative control) - 0.0033% final SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies IL7: Interleukin-7 based stimulation DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM |
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| Experimental Condition | Log2 Fold Change | P value | Adjusted P value |
| AZA vs. CD3 | -0.0282013941708073 | 0.931992701997708 | 0.956293417149531 |
| AZA vs. DISU | -0.165934110271375 | 0.556045964757107 | 0.948689448488927 |
| AZA vs. IL7 | -0.129158764314861 | 0.558457953098036 | 0.999311006273513 |
| AZA vs. SAHA | -1.45618570677766 | 0.0480516883065129 | 0.263969077817921 |
| DISU vs. CD3 | -0.149724487128152 | 0.681821278174306 | 0.775915291197304 |
| DISU vs. IL7 | 0.0258763132817993 | 0.928146292433828 | 0.986449898367171 |
| DISU vs. SAHA | -1.28649504478268 | 0.0994694564796678 | 0.403853496391453 |
| DMSO vs. AZA | 0.0358599572258648 | 0.856066519361966 | 1 |
| DMSO vs. CD3 | 0.0549433501788615 | 0.864699672521987 | 0.907341406041912 |
| DMSO vs. DISU | 0.200100171598558 | 0.46149208154347 | 0.892965804867364 |
| DMSO vs. IL7 | -0.15786991377897 | 0.447651056174309 | 0.868701134765033 |
| DMSO vs. SAHA | -1.49704186784191 | 0.0415804752069278 | 0.227462556023088 |
| HIV vs. Mock in Activation | 0.21524865390716 | 0.73216730460406 | 0.999983755607037 |
| HIV vs. Mock in Latency | 0.153432637927753 | 0.369709754806894 | 0.999834320637052 |
| IL7 vs. CD3 | -0.0972578662544574 | 0.764590347199929 | 0.843741877395456 |
| SAHA vs. CD3 | -1.44988347088837 | 0.0484401252646833 | 0.0964068525803985 |
| SAHA vs. IL7 | -1.32771891042166 | 0.0675179387474465 | 0.224226791953956 |
| (2). Iglesias-Ussel et al., J. Virol., 2013 Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model |
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| Log2 Fold Change | P Value | ||
| unknown | unknown | ||
Gene expression during HIV infection and replication
| (1). Imbeault et al., PloS Pathog., 2012 Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells |
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| Experiment type | Log2 Fold Change | P Value | Adjusted P Value | ||
| Infected vs. Mock | unknown | unknown | unknown | ||
| Infected vs. Bystander | unknown | unknown | unknown | ||
| (2). Lefebvre et al., J. Virol., 2011 Transcriptome analysis of T-cell line (Sup T1) |
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| Log2 Fold Change | unknown | ||||
| (3). Li et al., J. Immunol., 2013 Lymphatic tissue |
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| Acute Fold Change | Acute P Value | Asymt Fold Change | Asypt P Value | AIDS Fold Change | AIDS P Value |
| unknown | unknown | unknown | unknown | unknown | unknown |
| (4). Chang et al., MBio., 2011 Transcriptome analysis of T-cell line (Sup T1) Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation |
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| Up-regulated (True) | TRUE | ||||
| (5). Sherrill-Mix et al., BMC Retrovirol., 2015 Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based |
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| Test Status | Log2 Fold Change | P Value | |||
| OK | 0.15183 | 0.458239 | |||
| (6). Rotger et al., PLoS Pathog., 2010 Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient (Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach) |
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| Log2 Fold Change | P Value | ||||
| unknown | unknown | ||||
Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV
| (1). Greenwood et al., Elife, 2016 Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset |
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| 6 h | 24 h | 48 h | 72 h | RTi | ||
| unknown | unknown | unknown | unknown | unknown | ||
| (2). Navare et al., Virology, 2012 SUP-T1 cell line |
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| FC-4hpi | P-value | FC-8hpi | P-value | FC-20hpi | P-value | Category |
| unknown | unknown | unknown | unknown | unknown | unknown | unknown |
| (3). Hyrcza et al., J. Virolo., 2007 Primary human CD4+ and CD8+ T Cells |
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| Affymetrix Prob ID | Fold Change | In CD8? | Category | |||
| unknown | unknown | unknown | unknown | |||
Protein Overview top
Drug-protein Interaction (annotations from DrugBank) top
Protein Secondary Structure (annotations from PDB) top
| PDB Accession | Method | Resolution | Chain | Structure Preview |
|---|---|---|---|---|
| 2GZV | X-ray | 1.1Å | A=19-105. |  |
HIV-1 Interaction (annotations from NCBI HIV-1 Interaction Database) top
Metabolic/Signalling Pathway (annotations from KEGG database) top
| Pathway Accession Number | Description | not found |
|---|