Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002169
UniProt IDQ9Y5J5
Primary gene name(s)PHLDA3
Synonym gene name(s)TIH1
Protein namePleckstrin homology-like domain family A member 3
Protein functionp53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its direct transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May act as a tumor suppressor. {ECO:0000269|PubMed:19203586}.
Subcellular locationCytoplasm {ECO:0000269|PubMed:19203586}. Membrane {ECO:0000269|PubMed:19203586};
Peripheral membrane protein {ECO:0000269|PubMed:19203586}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9Y5J5
Gene Ontology
(Biological Process)
Complete annatation
anatomical structure morphogenesis [GO:0009653];
intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator [GO:0042771];
negative regulation of protein kinase B signaling [GO:0051898];
positive regulation of apoptotic process [GO:0043065]
Gene Ontology
(Molecular Function)
Complete annatation
phosphatidylinositol-3,4,5-trisphosphate binding [GO:0005547];
phosphatidylinositol-3,4-bisphosphate binding [GO:0043325];
phosphatidylinositol-3,5-bisphosphate binding [GO:0080025];
phosphatidylinositol-3-phosphate binding [GO:0032266];
phosphatidylinositol-4,5-bisphosphate binding [GO:0005546];
phosphatidylinositol-5-phosphate binding [GO:0010314]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
extracellular exosome [GO:0070062];
plasma membrane [GO:0005886]
Protein-protein interaction117145
Phylogenetic treeQ9Y5J5
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.6855023683868030.0385490272071930.0802677248053182
AZA vs. DISU-0.7082812609711790.03164261787078330.419877483964953
AZA vs. IL7-0.388457981334040.2064489633691770.970014097526061
AZA vs. SAHA-1.773358053663070.0004934001265657350.0127792328313606
DISU vs. CD3-0.03388382233983530.9258050510413660.95151740516509
DISU vs. IL70.3094896745020870.3648055949868690.742265399436774
DISU vs. SAHA-1.06167618027870.03993464373567370.245154792562256
DMSO vs. AZA-0.6143173146857060.00743207216544851
DMSO vs. CD30.06115016142493010.8499446204497750.896793367913983
DMSO vs. DISU0.09259697335680650.7388893140051970.967837575700161
DMSO vs. IL70.2328537681564120.3552055359212810.819978686610572
DMSO vs. SAHA-1.162810133252560.01336199277257750.108853293554333
HIV vs. Mock in Activation0.12689269040390.8395088813359810.999983755607037
HIV vs. Mock in Latency-0.14739661135330.5840737024076240.999834320637052
IL7 vs. CD30.3012662458319520.3509824042151510.48808541182166
SAHA vs. CD3-1.108932630650870.02298479502570730.0517882399824298
SAHA vs. IL7-1.385051173482430.006149827013496760.0448033506366621
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
0.9398 0.005824

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change 2.2210982
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 1.13025 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found