Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002168
UniProt IDP46019
Primary gene name(s)PHKA2
Synonym gene name(s)PHKLA, PYK
Protein namePhosphorylase b kinase regulatory subunit alpha, liver isoform
Protein functionPhosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin.
Subcellular locationCell membrane {ECO:0000305};
Lipid-anchor {ECO:0000305};
Cytoplasmic side {ECO:0000305}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P46019
Gene Ontology
(Biological Process)
Complete annatation
carbohydrate metabolic process [GO:0005975];
cellular protein modification process [GO:0006464];
generation of precursor metabolites and energy [GO:0006091];
glycogen catabolic process [GO:0005980]
Gene Ontology
(Molecular Function)
Complete annatation
phosphorylase kinase activity [GO:0004689]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
phosphorylase kinase complex [GO:0005964];
plasma membrane [GO:0005886]
Protein-protein interaction111274
Phylogenetic treeP46019
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.462356618731860.1605152218648750.2568243549838
AZA vs. DISU0.2333736344500910.3603553356594190.893849883358823
AZA vs. IL7-0.1502519736211350.4381813932727860.999311006273513
AZA vs. SAHA0.0567594287617330.8167555597877960.95358789468215
DISU vs. CD30.6824721799765210.06491157210566820.133071812582734
DISU vs. IL7-0.3929167215455890.122437814745410.458343694384667
DISU vs. SAHA-0.1741988459739670.5531945202796370.85273546232325
DMSO vs. AZA0.09511390289986820.5739672674740081
DMSO vs. CD30.5445341088497350.09058351521134080.157634360247066
DMSO vs. DISU-0.1403590773382470.568541567505120.928771969897253
DMSO vs. IL7-0.237936423273310.1893813161931340.700391485748232
DMSO vs. SAHA-0.04365436027512330.8536039428714930.961832482196079
HIV vs. Mock in Activation0.1878576650258930.7631848640263480.999983755607037
HIV vs. Mock in Latency0.06949807997502060.6760803230417730.999834320637052
IL7 vs. CD30.3179860574457290.3248503304835770.462944033364337
SAHA vs. CD30.4955516807094250.1628842956392270.256925511333803
SAHA vs. IL70.2039986215395210.4042533236048180.650551361163556
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.51473 0.000580925
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.059 0.918 1.034 1.071 1.042
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
retropepsin cleaves 1872871

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04020 Calcium signaling pathway - Homo sapiens (human)
hsa04910 Insulin signaling pathway - Homo sapiens (human)
hsa04922 Glucagon signaling pathway - Homo sapiens (human)