Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002162
UniProt IDQ96BD5
Primary gene name(s)PHF21A
Synonym gene name(s)BHC80, KIAA1696
Protein namePHD finger protein 21A
Protein functionComponent of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it may act as a scaffold. Inhibits KDM1A-mediated demethylation of 'Lys-4' of histone H3 in vitro, suggesting a role in demethylation regulation. {ECO:0000269|PubMed:16140033}.
Subcellular locationNucleus {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q96BD5
Gene Ontology
(Biological Process)
Complete annatation
blood coagulation [GO:0007596];
negative regulation of transcription from RNA polymerase II promoter [GO:0000122];
regulation of transcription, DNA-templated [GO:0006355];
transcription, DNA-templated [GO:0006351]
Gene Ontology
(Molecular Function)
Complete annatation
chromatin binding [GO:0003682];
histone binding [GO:0042393];
histone deacetylase activity [GO:0004407];
RNA polymerase II regulatory region sequence-specific DNA binding [GO:0000977];
transcription cofactor activity [GO:0003712];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
DNA repair complex [GO:1990391];
histone deacetylase complex [GO:0000118];
nucleoplasm [GO:0005654]
Protein-protein interaction119468
Phylogenetic treeQ96BD5
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.5846525594404550.07558214786422070.139741674755831
AZA vs. DISU0.2464341048209520.3314696457254030.879368785579854
AZA vs. IL7-0.2734407376343880.1576512210843770.922406965798721
AZA vs. SAHA0.3280272417399120.1801909282401460.541580588230571
DISU vs. CD30.8182756439256510.0249214789070260.0615644662478712
DISU vs. IL7-0.5293090348592980.03645617396249710.249694149243364
DISU vs. SAHA0.083570439222020.7743445009020780.937083566082913
DMSO vs. AZA0.02998669074512460.8588319155335011
DMSO vs. CD30.6038813841424730.06016053516918150.11246041225012
DMSO vs. DISU-0.218039254993980.372783996315550.852611143492993
DMSO vs. IL7-0.296325210755180.1016134064786350.58045551446208
DMSO vs. SAHA0.2916558038716570.2172756670319420.560808627819558
HIV vs. Mock in Activation0.2062060530610.7407217960259290.999983755607037
HIV vs. Mock in Latency0.08184917577940980.6208208961209140.999834320637052
IL7 vs. CD30.3175957047218360.3238873752427360.461956321634534
SAHA vs. CD30.8882260967393690.01261556355747240.0311819817633441
SAHA vs. IL70.5980770803227640.01457884808263630.0813630344308256
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.341704 0.00945105
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2PUY X-ray 1.4Å A/B=486-543.
2YQL NMR - A=487-535.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found