Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002154
UniProt IDQ99623
Primary gene name(s)PHB2
Synonym gene name(s)BAP, REA
Protein nameProhibitin-2
Protein functionActs as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases, By similarity. Functions as an estrogen receptor, ER-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. Competes with NCOA1 for modulation of ER transcriptional activity. Probably involved in regulating mitochondrial respiration activity and in aging. {ECO:0000250|UniProtKB:O35129, ECO:0000269|PubMed:10359819, ECO:0000303|PubMed:11302691}.
Subcellular locationMitochondrion inner membrane {ECO:0000250|UniProtKB:O35129}. Cytoplasm {ECO:0000269|PubMed:19496786}. Nucleus {ECO:0000269|PubMed:19496786}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q99623
Gene Ontology
(Biological Process)
Complete annatation
mammary gland alveolus development [GO:0060749];
mammary gland branching involved in thelarche [GO:0060744];
mitochondrion organization [GO:0007005];
negative regulation of apoptotic process [GO:0043066];
negative regulation of intracellular estrogen receptor signaling pathway [GO:0033147];
negative regulation of mammary gland epithelial cell proliferation [GO:0033600];
negative regulation of sequence-specific DNA binding transcription factor activity [GO:0043433];
negative regulation of transcription, DNA-templated [GO:0045892];
positive regulation of cell cycle G1/S phase transition [GO:1902808];
positive regulation of ERK1 and ERK2 cascade [GO:0070374];
positive regulation of exit from mitosis [GO:0031536];
positive regulation of sequence-specific DNA binding transcription factor activity [GO:0051091];
protein import into nucleus, translocation [GO:0000060];
protein stabilization [GO:0050821];
regulation of branching involved in mammary gland duct morphogenesis [GO:0060762];
sister chromatid cohesion [GO:0007062];
transcription, DNA-templated [GO:0006351]
Gene Ontology
(Molecular Function)
Complete annatation
amide binding [GO:0033218];
estrogen receptor binding [GO:0030331];
protein C-terminus binding [GO:0008022];
protein N-terminus binding [GO:0047485]
Gene Ontology
(Cellular Component)
Complete annatation
cell periphery [GO:0071944];
cell surface [GO:0009986];
cytoplasm [GO:0005737];
extracellular exosome [GO:0070062];
mitochondrial inner membrane [GO:0005743];
mitochondrial outer membrane [GO:0005741];
mitochondrion [GO:0005739];
nuclear matrix [GO:0016363];
nucleus [GO:0005634];
protein complex [GO:0043234]
Protein-protein interaction116459
Phylogenetic treeQ99623
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.270388218750090.0001298372047265910.000609017679171468
AZA vs. DISU-0.1642169149293130.5157667693207540.938437432475966
AZA vs. IL70.02651730862585910.8901782602004520.999311006273513
AZA vs. SAHA0.280953169799280.2490018311516590.619540077896255
DISU vs. CD3-1.447299174461148.88503558964526e-050.000511389944552549
DISU vs. IL70.1815543019985490.4706299880918570.808333949012732
DISU vs. SAHA0.4470662596552370.1251636037399320.454868741732462
DMSO vs. AZA-0.05878551859108530.7252042893928361
DMSO vs. CD3-1.343177873613253.53863159184842e-050.000178054391887764
DMSO vs. DISU0.1028032794101170.6731364727743580.95498935710817
DMSO vs. IL70.09295838308308440.6047996420068080.913200494209438
DMSO vs. SAHA0.3342640489299730.1559726741122710.476393680446396
HIV vs. Mock in Activation-0.115571160590880.8529645991720870.999983755607037
HIV vs. Mock in Latency-0.1783017826172070.2789817608174160.999834320637052
IL7 vs. CD3-1.236611510393460.0001476949758687510.000821323241870804
SAHA vs. CD3-1.013837843074470.004420203013415060.0128620311666663
SAHA vs. IL70.2513465248225910.3018334200621490.550561105277932
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.216461 0.117174
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.026 0.926 1.017 1.031 0.858
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp160; precursor binds 19906925
Envelope surface glycoprotein gp120 complexes with 23125841
Gag-Pol complexes with 23125841
Envelope transmembrane glycoprotein gp41 interacts with 22190034
Pr55(Gag) complexes with 23125841
Nef complexes with 23125841

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found