Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002148
UniProt IDP00558
Primary gene name(s)PGK1
Synonym gene name(s)PGKA
Protein namePhosphoglycerate kinase 1
Protein functionIn addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein, primer recognition protein, PubMed:2324090. May play a role in sperm motility, PubMed:26677959. {ECO:0000269|PubMed:2324090, ECO:0000269|PubMed:26677959}.
Subcellular locationCytoplasm.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P00558
Gene Ontology
(Biological Process)
Complete annatation
canonical glycolysis [GO:0061621];
epithelial cell differentiation [GO:0030855];
gluconeogenesis [GO:0006094];
phosphorylation [GO:0016310]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
phosphoglycerate kinase activity [GO:0004618]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
extracellular exosome [GO:0070062];
membrane [GO:0016020];
membrane raft [GO:0045121]
Protein-protein interaction111251
Phylogenetic treeP00558
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.8625226183549110.009000241237560270.0238823302698078
AZA vs. DISU-0.1188587293528880.6388274426438540.960057478314726
AZA vs. IL70.4427299931438090.02099429693531980.464059243345433
AZA vs. SAHA0.2209428787322970.3661379742801280.733233101034296
DISU vs. CD3-0.994161479056040.007247242598705790.0221824046881221
DISU vs. IL70.5524632756847820.02889223684894660.218869583164759
DISU vs. SAHA0.3417613536569030.2477479614604850.625638325598352
DMSO vs. AZA0.006653767433663240.9681520424382361
DMSO vs. CD3-0.8678290187802220.007121266687462490.0185930472524476
DMSO vs. DISU0.1235124814345640.6131158433386870.939289650701039
DMSO vs. IL70.4434287929000660.01343154537603340.244246738894697
DMSO vs. SAHA0.2082804310663730.3780492092180740.725713874936389
HIV vs. Mock in Activation0.004353323352444530.994408372213480.999983755607037
HIV vs. Mock in Latency0.03403791669974120.9106495893800870.999834320637052
IL7 vs. CD3-0.4125822124571580.200241555562930.322737485778448
SAHA vs. CD3-0.665621673664820.06441738598043820.122201662477996
SAHA vs. IL7-0.2243796935032890.3579363047655320.608564387326374
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.00517493 0.979919
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.057 0.857 0.87 0.861 0.722
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB03909 Adenosine-5&,39;-[Beta, Gamma-Methylene]Triphosphate experimental unknown unknown
DB04510 3-Phosphoglyceric Acid experimental unknown unknown
DB00709 Lamivudine approved, investigational unknown substrate

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2WZB X-ray 1.4Å A=2-417.
2WZC X-ray 1.5Å A=2-417.
2WZD X-ray 1.5Å A=1-417.
2X13 X-ray 1.7Å A=2-417.
2X14 X-ray 1.9Å A=2-417.
2X15 X-ray 2.1Å A=2-417.
2XE6 X-ray 1.7Å A=1-417.
2XE7 X-ray 2.2Å A=1-417.
2XE8 X-ray 1.7Å A=1-417.
2Y3I X-ray 2.9Å A/D=1-416.
2YBE X-ray 2.0Å A=1-417.
2ZGV X-ray 2.0Å A=1-417.
3C39 X-ray 1.8Å A/B=1-417.
3C3A X-ray 2.3Å A/B=1-417.
3C3B X-ray 1.8Å A/B=1-417.
3C3C X-ray 2.4Å A/B=1-417.
3ZOZ X-ray 1.9Å A=1-417.
4AXX X-ray 1.7Å A=1-417.
4O33 X-ray 2.1Å A=1-417.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat upregulates 23166591

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00010 Glycolysis / Gluconeogenesis - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa01200 Carbon metabolism - Homo sapiens (human)
hsa01230 Biosynthesis of amino acids - Homo sapiens (human)
hsa04066 HIF-1 signaling pathway - Homo sapiens (human)