Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002144
UniProt IDQ96HS1
Primary gene name(s)PGAM5
Synonym gene name(s)unknown
Protein nameSerine/threonine-protein phosphatase PGAM5, mitochondrial
Protein functionDisplays phosphatase activity for serine/threonine residues, and, dephosphorylates and activates MAP3K5 kinase. Has apparently no phosphoglycerate mutase activity. May be regulator of mitochondrial dynamics. Substrate for a KEAP1-dependent ubiquitin ligase complex. Contributes to the repression of NFE2L2-dependent gene expression. Acts as a central mediator for programmed necrosis induced by TNF, by reactive oxygen species and by calcium ionophore. {ECO:0000269|PubMed:18387606, ECO:0000269|PubMed:19590015, ECO:0000269|PubMed:22265414}.
Subcellular locationMitochondrion outer membrane {ECO:0000269|PubMed:18387606};
Single-pass membrane protein {ECO:0000269|PubMed:18387606}. Note=Isoform 2 overexpression results in the formation of disconnected punctuate mitochondria distributed throughout the cytoplasm. Isoform 1 overexpression results in the clustering of mitochondria around the nucleus.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q96HS1
Gene Ontology
(Biological Process)
Complete annatation
necroptotic process [GO:0070266]
Gene Ontology
(Molecular Function)
Complete annatation
GTPase activator activity [GO:0005096];
phosphatase activity [GO:0016791];
protein complex binding [GO:0032403];
receptor signaling protein serine/threonine phosphatase activity [GO:0009400]
Gene Ontology
(Cellular Component)
Complete annatation
integral component of membrane [GO:0016021];
mitochondrial outer membrane [GO:0005741];
mitochondrion [GO:0005739]
Protein-protein interaction128154
Phylogenetic treeQ96HS1
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.419918588482252.09481573635317e-050.000120049668358612
AZA vs. DISU-0.04806662585421130.8499427187705290.988692756671169
AZA vs. IL70.2475745200655210.2012224242726930.965934195269828
AZA vs. SAHA-0.2811143947763130.2521255851335090.6223253758478
DISU vs. CD3-1.479757325603096.33759291230884e-050.000380306033280826
DISU vs. IL70.2863997812804790.2576645649380050.643196646390717
DISU vs. SAHA-0.2314674197953470.4287062813616520.777431119390744
DMSO vs. AZA0.03914812733472550.8172188468856571
DMSO vs. CD3-1.393631593924191.87155496735869e-050.000101093494382426
DMSO vs. DISU0.0850264029723230.728543379106530.965539418092991
DMSO vs. IL70.2159728699509420.2331974047759070.741355029737196
DMSO vs. SAHA-0.3258461797948780.1695041545717560.497677926104274
HIV vs. Mock in Activation-0.05079644453301740.9349114362689640.999983755607037
HIV vs. Mock in Latency-0.1224656292339330.4641726524100060.999834320637052
IL7 vs. CD3-1.165340442831390.0003411302181438590.00168419776550357
SAHA vs. CD3-1.725330709490672.02377322322533e-061.5341178306844e-05
SAHA vs. IL7-0.5318207341441920.03010458944762320.131041461545906
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
-0.421 0.02104

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.24285 0.117135
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.987 0.947 0.965 0.88 0.904
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3MXO X-ray 1.7Å A/B=90-289.
3O0T X-ray 1.9Å A/B=90-289.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04668 TNF signaling pathway - Homo sapiens (human)