Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002138
UniProt IDP50542
Primary gene name(s)PEX5
Synonym gene name(s)PXR1
Protein namePeroxisomal targeting signal 1 receptor
Protein functionBinds to the C-terminal PTS1-type tripeptide peroxisomal targeting signal, SKL-type and plays an essential role in peroxisomal protein import. {ECO:0000269|PubMed:7706321, ECO:0000269|PubMed:7719337, ECO:0000269|PubMed:7790377}.
Subcellular locationCytoplasm. Peroxisome membrane;
Peripheral membrane protein. Note=Its distribution appears to be dynamic. It is probably a cycling receptor found mainly in the cytoplasm and as well associated to the peroxisomal membrane through a docking factor, PEX13.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P50542
Gene Ontology
(Biological Process)
Complete annatation
negative regulation of protein homotetramerization [GO:1901094];
protein import into peroxisome matrix [GO:0016558];
protein import into peroxisome matrix, docking [GO:0016560];
protein import into peroxisome matrix, translocation [GO:0016561];
protein import into peroxisome membrane [GO:0045046];
protein targeting to peroxisome [GO:0006625];
protein tetramerization [GO:0051262]
Gene Ontology
(Molecular Function)
Complete annatation
enzyme binding [GO:0019899];
peroxisome matrix targeting signal-1 binding [GO:0005052];
peroxisome targeting sequence binding [GO:0000268];
protein C-terminus binding [GO:0008022];
protein N-terminus binding [GO:0047485];
small GTPase binding [GO:0031267]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
intracellular [GO:0005622];
membrane [GO:0016020];
peroxisomal matrix [GO:0005782];
peroxisomal membrane [GO:0005778];
peroxisome [GO:0005777];
protein complex [GO:0043234]
Protein-protein interaction111788
Phylogenetic treeP50542
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.9983311760263440.0913808965616910.163515715656869
AZA vs. DISU-0.1222893278536450.6318751205825270.960057478314726
AZA vs. IL70.1539944568657570.4281343297445290.999311006273513
AZA vs. SAHA0.3357807230871820.1758890616561180.535275320388815
DISU vs. CD3-1.132971417315780.06182601982829290.128105212768617
DISU vs. IL70.2669191742460510.2943399826165060.678373952199046
DISU vs. SAHA0.4602141276087130.120279189788650.445059579991345
DMSO vs. AZA0.06351467632500950.7088449361638931
DMSO vs. CD3-0.9475296839611680.1064195865291580.179732182854458
DMSO vs. DISU0.1836833614782880.4552989785608650.890202310783654
DMSO vs. IL70.09808480431967920.5894725867209590.909845858619163
DMSO vs. SAHA0.2669569073854560.2617943840884530.611231551106784
HIV vs. Mock in Activation0.06195392878169710.9601234866427370.999983755607037
HIV vs. Mock in Latency-0.03894147834860980.8165814906056630.999834320637052
IL7 vs. CD3-0.8385223569982450.1536857616848350.263959148344977
SAHA vs. CD3-0.6865778519068480.255810566608370.366040315738734
SAHA vs. IL70.1793541867307350.4876019967200290.718767113099718
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.182769 0.227286
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.797 0.904 0.847 0.89 0.997
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1FCH X-ray 2.2Å A/B=272-639.
2C0L X-ray 2.3Å A=335-639.
2C0M X-ray 2.5Å A/B/C/F=321-639.
2J9Q X-ray 2.6Å A/B=315-639.
2W84 NMR - B=108-127.
3R9A X-ray 2.3Å B/D=315-639.
4BXU NMR - B=57-71.
4KXK X-ray 2.9Å B/D=315-639.
4KYO X-ray 2.2Å B/D=315-639.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04146 Peroxisome - Homo sapiens (human)