Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002132
UniProt IDQ96GD0
Primary gene name(s)PDXP
Synonym gene name(s)CIN, PLP, PLPP
Protein namePyridoxal phosphate phosphatase
Protein functionProtein serine phosphatase that dephosphorylates 'Ser-3' in cofilin and probably also dephosphorylates phospho-serine residues in DSTN. Regulates cofilin-dependent actin cytoskeleton reorganization. Required for normal progress through mitosis and normal cytokinesis. Does not dephosphorylate phospho-threonines in LIMK1. Does not dephosphorylate peptides containing phospho-tyrosine. Pyridoxal phosphate phosphatase. Has some activity towards pyridoxal 5'-phosphate, PLP, pyridoxine 5'-phosphate, PMP and pyridoxine 5'-phosphate, PNP, with a highest activity with PLP followed by PNP. {ECO:0000269|PubMed:14522954, ECO:0000269|PubMed:15580268}.
Subcellular locationCytoplasm, cytosol {ECO:0000269|PubMed:15580268}. Cytoplasm, cytoskeleton {ECO:0000269|PubMed:15580268}. Cell projection, ruffle membrane {ECO:0000269|PubMed:15580268};
Peripheral membrane protein {ECO:0000269|PubMed:15580268};
Cytoplasmic side {ECO:0000269|PubMed:15580268}. Cell projection, lamellipodium membrane {ECO:0000269|PubMed:15580268};
Peripheral membrane protein {ECO:0000269|PubMed:15580268};
Cytoplasmic side {ECO:0000269|PubMed:15580268}. Cell membrane {ECO:0000269|PubMed:15580268};
Peripheral membrane protein {ECO:0000269|PubMed:15580268};
Cytoplasmic side {ECO:0000269|PubMed:15580268}. Note=Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Diffusely distributed throughout the cytosol during pro-metaphase and metaphase. Detected at the dynamic cell poles during telophase. Detected at the cleavage furrow and contractile ring during cytokinesis. Transiently detected at the plasma membrane in late stages of cytokinesis. Detected at the midbody. {ECO:0000269|PubMed:15580268}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q96GD0
Gene Ontology
(Biological Process)
Complete annatation
actin rod assembly [GO:0031247];
cellular response to ATP [GO:0071318];
positive regulation of actin filament depolymerization [GO:0030836];
protein dephosphorylation [GO:0006470];
pyridoxal phosphate catabolic process [GO:0032361];
regulation of cytokinesis [GO:0032465];
regulation of mitotic nuclear division [GO:0007088]
Gene Ontology
(Molecular Function)
Complete annatation
heat shock protein binding [GO:0031072];
magnesium ion binding [GO:0000287];
phosphoprotein phosphatase activity [GO:0004721];
phosphoserine phosphatase activity [GO:0004647];
pyridoxal phosphatase activity [GO:0033883]
Gene Ontology
(Cellular Component)
Complete annatation
cytoskeleton [GO:0005856];
cytosol [GO:0005829];
extracellular exosome [GO:0070062];
lamellipodium membrane [GO:0031258];
ruffle membrane [GO:0032587]
Protein-protein interaction121330
Phylogenetic treeQ96GD0
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.7836618767607910.01904111916710870.0447021414465206
AZA vs. DISU-0.1920919885961540.4589918140069550.923374163131035
AZA vs. IL70.09251505108906510.6430077427237030.999311006273513
AZA vs. SAHA-0.2661092412721610.2888893166240510.661026159742352
DISU vs. CD3-0.9876483721089530.007309776588258820.0223142106705981
DISU vs. IL70.2749932783061640.2857407848488170.672843225803799
DISU vs. SAHA-0.07174750696450820.8098912563421710.950589679896226
DMSO vs. AZA-0.01101022674297830.95027694941161
DMSO vs. CD3-0.8082666373603890.01319731240358340.0315483197968872
DMSO vs. DISU0.178698298467480.4748565826558190.898216229524689
DMSO vs. IL70.1112767836791130.5516867868109260.898517535779463
DMSO vs. SAHA-0.2594511401433270.2842558399839340.634585512654582
HIV vs. Mock in Activation0.232706500435930.7098285415874070.999983755607037
HIV vs. Mock in Latency-0.09620002751333110.5827612631134710.999834320637052
IL7 vs. CD3-0.6856908448870130.03574782376584520.0837858727449206
SAHA vs. CD3-1.073228598876890.003166878754786720.0096297140505722
SAHA vs. IL7-0.3605107202978690.149436455860240.365675517248203
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.337478 0.0108507
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.013 0.879 1.022 1.072 0.977
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00114 Pyridoxal Phosphate nutraceutical unknown cofactor

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2CFR X-ray 2.4Å A=1-296.
2CFS X-ray 2.4Å A=1-296.
2CFT X-ray 1.8Å A=1-296.
2OYC X-ray 1.7Å A=2-296.
2P27 X-ray 1.9Å A=2-296.
2P69 X-ray 2.2Å A=2-296.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00750 Vitamin B6 metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)