Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002131
UniProt IDO00764
Primary gene name(s)PDXK
Synonym gene name(s)C21orf124, C21orf97, PKH, PNK
Protein namePyridoxal kinase
Protein functionRequired for synthesis of pyridoxal-5-phosphate from vitamin B6.
Subcellular locationCytoplasm.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O00764
Gene Ontology
(Biological Process)
Complete annatation
cell proliferation [GO:0008283];
pyridoxal 5'-phosphate salvage [GO:0009443];
pyridoxal phosphate biosynthetic process [GO:0042823];
vitamin B6 metabolic process [GO:0042816]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
lithium ion binding [GO:0031403];
magnesium ion binding [GO:0000287];
potassium ion binding [GO:0030955];
protein homodimerization activity [GO:0042803];
pyridoxal kinase activity [GO:0008478];
pyridoxal phosphate binding [GO:0030170];
sodium ion binding [GO:0031402];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
extracellular exosome [GO:0070062];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction114135
Phylogenetic treeO00764
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.2411096954800680.4624431048542280.582252926544168
AZA vs. DISU0.2924132821209860.2498160919279630.831286483382367
AZA vs. IL7-0.01603402377889330.9335765800835470.999311006273513
AZA vs. SAHA-0.2133097613784770.3822611350012580.746235160409375
DISU vs. CD30.03914071366652670.9150585858684020.945025563399582
DISU vs. IL7-0.3175387560280810.2094057310339150.588456126390143
DISU vs. SAHA-0.5039756279535990.08513638824679910.371888474921146
DMSO vs. AZA-0.00570438946172420.9728733057108471
DMSO vs. CD3-0.2591882140129890.4190800618758860.533410031443074
DMSO vs. DISU-0.3002569877808310.220067076304670.732261170216687
DMSO vs. IL7-0.002790615011004470.9876260810001550.998499429186965
DMSO vs. SAHA-0.2135601931479030.3652621443957540.713250295178596
HIV vs. Mock in Activation-0.02045531607154690.9737703046088550.999983755607037
HIV vs. Mock in Latency-0.04287350484776540.7950560612671290.999834320637052
IL7 vs. CD3-0.2500425799195110.4376225467265230.572345739419425
SAHA vs. CD3-0.4785960914595530.1776321191379530.27560136264892
SAHA vs. IL7-0.2003670138471750.4108380202866020.65663356824132
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.337255 0.0135765
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.964 0.942 1.076 1.27 0.961
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
-1.03 <0.0001 -0.23 0.0716 0.27 0.3604 Cell growth and proliferation at 4 hpi
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00147 Pyridoxal approved, nutraceutical yes unknown
DB00165 Pyridoxine approved, nutraceutical, vet_approved yes ligand
DB03909 Adenosine-5&,39;-[Beta, Gamma-Methylene]Triphosphate experimental unknown unknown
DB04770 O6-(R)-ROSCOVITINE, R-2-(6-BENZYLOXY-9-ISOPROPYL-9H-PURIN-2-YLAMINO)-BUTAN-1-OL experimental unknown unknown
DB04776 (2R)-2-({6-[benzyl(methyl)amino]-9-isopropyl-9H-purin-2-yl}amino)butan-1-ol experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2AJP X-ray 2.5Å A/B=6-312.
2F7K X-ray 2.8Å A/B=1-312.
2YXT X-ray 2.0Å A/B=1-312.
2YXU X-ray 2.2Å A/B=1-312.
3FHX X-ray 2.5Å A/B=1-312.
3FHY X-ray 2.3Å A/B=1-312.
3KEU X-ray 2.1Å A/B=1-312.
4EN4 X-ray 2.1Å A/B=1-312.
4EOH X-ray 2.1Å A/B=1-312.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00750 Vitamin B6 metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)