Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002128
UniProt IDQ9NR12
Primary gene name(s)PDLIM7
Synonym gene name(s)ENIGMA
Protein namePDZ and LIM domain protein 7
Protein functionMay function as a scaffold on which the coordinated assembly of proteins can occur. May play a role as an adapter that, via its PDZ domain, localizes LIM-binding proteins to actin filaments of both skeletal muscle and nonmuscle tissues. Involved in both of the two fundamental mechanisms of bone formation, direct bone formation, e.g. embryonic flat bones mandible and cranium, and endochondral bone formation, e.g. embryonic long bone development. Plays a role during fracture repair. Involved in BMP6 signaling pathway, By similarity. {ECO:0000250, ECO:0000269|PubMed:11874232, ECO:0000269|PubMed:7929196}.
Subcellular locationCytoplasm {ECO:0000250}. Cytoplasm, cytoskeleton {ECO:0000250}. Note=Colocalizes with RET to the cell periphery and in some cytoskeletal components. Colocalizes with TPM2 near the Z line in muscle. Colocalizes with TBX4 and TBX5 to actin filaments, By similarity. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9NR12
Gene Ontology
(Biological Process)
Complete annatation
actin cytoskeleton organization [GO:0030036];
cell differentiation [GO:0030154];
multicellular organism development [GO:0007275];
ossification [GO:0001503];
positive regulation of osteoblast differentiation [GO:0045669];
receptor-mediated endocytosis [GO:0006898]
Gene Ontology
(Molecular Function)
Complete annatation
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
actin cytoskeleton [GO:0015629];
cytoplasm [GO:0005737];
focal adhesion [GO:0005925];
nucleus [GO:0005634];
ruffle [GO:0001726];
stress fiber [GO:0001725]
Protein-protein interaction114682
Phylogenetic treeQ9NR12
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.2606642993775680.4513121710799540.572236648651221
AZA vs. DISU0.1176053092715370.6550792150347130.960323430331341
AZA vs. IL7-0.009797197203041810.9619076760208590.999311006273513
AZA vs. SAHA0.6579481389170150.009441101469871430.0939994475276367
DISU vs. CD3-0.1575718324231890.7103472450297820.79688727927208
DISU vs. IL7-0.1385608636544950.6147383849653080.882840921534916
DISU vs. SAHA0.5435912811421380.06810569822481660.330038899661161
DMSO vs. AZA0.02800681249117020.8779340689649771
DMSO vs. CD3-0.2468671859837890.4674706790393640.578094846847776
DMSO vs. DISU-0.09276217302755070.7144712640590650.962685308322123
DMSO vs. IL7-0.02994008344870110.8761913005898750.97288360624247
DMSO vs. SAHA0.6254776601782830.01047603655085390.0924139386702092
HIV vs. Mock in Activation0.3579945267762870.6523653121037980.999983755607037
HIV vs. Mock in Latency-0.1862827496577040.2934966013299950.999834320637052
IL7 vs. CD3-0.2651650586523040.4603416960355090.593358165242281
SAHA vs. CD30.3734429438647110.3235220689077580.438481660080935
SAHA vs. IL70.6659682175615250.008646148014895720.0565431822891705
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.0123892 0.967726
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.007 1.008 0.905 1.009 1.024
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2Q3G X-ray 1.1Å A/B=1-84.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found