Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002127
UniProt IDQ15120
Primary gene name(s)PDK3
Synonym gene name(s)PDHK3
Protein name[Pyruvate dehydrogenase
Protein functionInhibits pyruvate dehydrogenase activity by phosphorylation of the E1 subunit PDHA1, and thereby regulates glucose metabolism and aerobic respiration. Can also phosphorylate PDHA2. Decreases glucose utilization and increases fat metabolism in response to prolonged fasting, and as adaptation to a high-fat diet. Plays a role in glucose homeostasis and in maintaining normal blood glucose levels in function of nutrient levels and under starvation. Plays a role in the generation of reactive oxygen species. {ECO:0000269|PubMed:10748134, ECO:0000269|PubMed:11486000, ECO:0000269|PubMed:15861126, ECO:0000269|PubMed:16436377, ECO:0000269|PubMed:17683942, ECO:0000269|PubMed:18718909, ECO:0000269|PubMed:22865452}.
Subcellular locationMitochondrion matrix.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q15120
Gene Ontology
(Biological Process)
Complete annatation
cellular response to fatty acid [GO:0071398];
cellular response to glucose stimulus [GO:0071333];
glucose metabolic process [GO:0006006];
hypoxia-inducible factor-1alpha signaling pathway [GO:0097411];
peptidyl-serine phosphorylation [GO:0018105];
peroxisome proliferator activated receptor signaling pathway [GO:0035357];
regulation of acetyl-CoA biosynthetic process from pyruvate [GO:0010510];
regulation of glucose metabolic process [GO:0010906];
regulation of reactive oxygen species metabolic process [GO:2000377]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
protein kinase activity [GO:0004672];
protein serine/threonine kinase activity [GO:0004674];
pyruvate dehydrogenase, acetyl-transferring kinase activity [GO:0004740]
Gene Ontology
(Cellular Component)
Complete annatation
mitochondrial matrix [GO:0005759];
mitochondrion [GO:0005739]
Protein-protein interaction111191
Phylogenetic treeQ15120
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.7009408635957310.1774436666121370.277978478658988
AZA vs. DISU0.1694903451758710.5112932007727140.938109377229618
AZA vs. IL7-0.07340587988508440.7138983970823520.999311006273513
AZA vs. SAHA0.1975222611629660.4300930667374820.779727356110615
DISU vs. CD30.857722418645290.09296123542186070.176020306839484
DISU vs. IL7-0.2513046570299150.3276691210284460.708086582497682
DISU vs. SAHA0.02928624446932820.9214287162311440.982027592272086
DMSO vs. AZA-0.02907930704009910.8691516456247091
DMSO vs. CD30.6605431780430680.2021679998967950.301789627273604
DMSO vs. DISU-0.2002257038437020.4211418650027780.877266532419223
DMSO vs. IL7-0.03744935550005820.8417973350390540.9650719278386
DMSO vs. SAHA0.2193471053177760.3642284482422170.712616765345768
HIV vs. Mock in Activation0.1415280108569180.8999971343442720.999983755607037
HIV vs. Mock in Latency0.04382096951355080.7997715507719020.999834320637052
IL7 vs. CD30.6373692708038660.2204557101283230.34733624660398
SAHA vs. CD30.8729870294059980.1232594211190540.206024786630894
SAHA vs. IL70.267006295383660.2877698782930920.534743977488914
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.659346 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.026 0.919 1.024 0.939 0.884
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB03758 Radicicol experimental unknown unknown
DB03760 Dihydrolipoic Acid experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1Y8N X-ray 2.6Å A=9-406.
1Y8O X-ray 2.4Å A=9-406.
1Y8P X-ray 2.6Å A=9-406.
2PNR X-ray 2.5Å A/B/E/F=9-406.
2Q8I X-ray 2.6Å A=9-406.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found