Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002122
UniProt IDP08559
Primary gene name(s)PDHA1
Synonym gene name(s)PHE1A
Protein namePyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial
Protein functionThe pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2, and thereby links the glycolytic pathway to the tricarboxylic cycle. {ECO:0000269|PubMed:19081061, ECO:0000269|PubMed:7782287}.
Subcellular locationMitochondrion matrix.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P08559
Gene Ontology
(Biological Process)
Complete annatation
acetyl-CoA biosynthetic process from pyruvate [GO:0006086];
glucose metabolic process [GO:0006006];
glyoxylate metabolic process [GO:0046487];
mitochondrial acetyl-CoA biosynthetic process from pyruvate [GO:0061732];
pyruvate metabolic process [GO:0006090];
regulation of acetyl-CoA biosynthetic process from pyruvate [GO:0010510];
tricarboxylic acid cycle [GO:0006099]
Gene Ontology
(Molecular Function)
Complete annatation
pyruvate dehydrogenase, acetyl-transferring activity [GO:0004739];
pyruvate dehydrogenase, NAD+ activity [GO:0034604];
pyruvate dehydrogenase activity [GO:0004738]
Gene Ontology
(Cellular Component)
Complete annatation
mitochondrial matrix [GO:0005759];
mitochondrion [GO:0005739];
myelin sheath [GO:0043209];
nucleus [GO:0005634];
pyruvate dehydrogenase complex [GO:0045254]
Protein-protein interaction111186
Phylogenetic treeP08559
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.019615832259240.002049358974864130.00675441471007047
AZA vs. DISU0.03804433703262630.8806984219103630.991771899371402
AZA vs. IL70.1450264607125090.4529132290785490.999311006273513
AZA vs. SAHA0.1464763304730910.5500314165182110.84724847461634
DISU vs. CD3-0.9944684183196620.006542974393680460.0203442235994924
DISU vs. IL70.09773558410515050.6985235128665580.922653286293118
DISU vs. SAHA0.1105667102588040.7051279601774750.91288546904155
DMSO vs. AZA0.03551181068772540.8333470737095171
DMSO vs. CD3-0.9953618172244480.002048741822632930.00637033964044686
DMSO vs. DISU-0.004147638415597040.986464158229810.997977034037055
DMSO vs. IL70.116548982486610.5189785800253510.889753450913474
DMSO vs. SAHA0.1049391913400840.6574182315994890.891884973304159
HIV vs. Mock in Activation0.08721013235508650.8884917304898810.999983755607037
HIV vs. Mock in Latency-0.007242349044717410.965256531579070.999834320637052
IL7 vs. CD3-0.8678359067911070.007297164532127030.0230249447336719
SAHA vs. CD3-0.8970787251143810.01198254440123390.0298869487134385
SAHA vs. IL7-0.0007138467433107020.9976715727188990.998625268251198
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.106725 0.511565
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.033 0.911 0.983 0.991 0.784
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00157 NADH nutraceutical unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1NI4 X-ray 1.9Å A/C=30-390.
2OZL X-ray 1.9Å A/C=30-390.
3EXE X-ray 1.9Å A/C/E/G=30-390.
3EXF X-ray 3.0Å A/C/E/G=30-390.
3EXG X-ray 3.0Å 1/3/5/A/C/E/G/I/K/M/O/Q/S/U/W/Y=30-390.
3EXH X-ray 2.4Å A/C/E/G=30-390.
3EXI X-ray 2.2Å A=30-390.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vpr induces phosphorylation of 23842279

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00010 Glycolysis / Gluconeogenesis - Homo sapiens (human)
hsa00020 Citrate cycle (TCA cycle) - Homo sapiens (human)
hsa00620 Pyruvate metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa01200 Carbon metabolism - Homo sapiens (human)
hsa04066 HIF-1 signaling pathway - Homo sapiens (human)
hsa04922 Glucagon signaling pathway - Homo sapiens (human)
hsa05230 Central carbon metabolism in cancer - Homo sapiens (human)