Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002121
UniProt IDO60658
Primary gene name(s)PDE8A
Synonym gene name(s)unknown
Protein nameHigh affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A
Protein functionHydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development. {ECO:0000269|PubMed:18983167}.
Subcellular locationunknown
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O60658
Gene Ontology
(Biological Process)
Complete annatation
cAMP catabolic process [GO:0006198];
cellular response to epidermal growth factor stimulus [GO:0071364];
cyclic nucleotide metabolic process [GO:0009187];
negative regulation of cell death [GO:0060548];
negative regulation of hydrogen peroxide-induced cell death [GO:1903206];
positive regulation of ERK1 and ERK2 cascade [GO:0070374];
positive regulation of protein phosphorylation [GO:0001934];
regulation of transcription, DNA-templated [GO:0006355];
signal transduction [GO:0007165]
Gene Ontology
(Molecular Function)
Complete annatation
3',5'-cyclic-AMP phosphodiesterase activity [GO:0004115];
3',5'-cyclic-nucleotide phosphodiesterase activity [GO:0004114];
metal ion binding [GO:0046872]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
extracellular exosome [GO:0070062]
Protein-protein interaction111177
Phylogenetic treeO60658
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      Yes - Two siRNA pools inhibit HIV replication and inhibition of Tat-mediated transactivation of the HIV LTR is also observed
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.5512125956264590.0950283538507410.168720385114662
AZA vs. DISU-0.02810909639108110.9126876428283940.994271142470137
AZA vs. IL70.1040937666616880.5974897109018350.999311006273513
AZA vs. SAHA0.2908904296140990.2402711965286780.611488775362705
DISU vs. CD3-0.5929075709837790.1042801788215570.192731659582656
DISU vs. IL70.123789793524160.6275534612245640.887792724020691
DISU vs. SAHA0.3201328563019690.2764306148021120.656246952222379
DMSO vs. AZA0.004791892485905650.9779586376030871
DMSO vs. CD3-0.558948280876530.08285541874575620.146533253117976
DMSO vs. DISU0.03105232742399280.9000501376139110.988283279918411
DMSO vs. IL70.1066268790530130.5632158330553310.902098850651192
DMSO vs. SAHA0.2792737913834910.243182906062120.591275817641076
HIV vs. Mock in Activation-0.1147927636691310.8540061489212320.999983755607037
HIV vs. Mock in Latency-0.2169172967377120.2011857905881940.999834320637052
IL7 vs. CD3-0.4385821118753050.1750476107604630.291675064889726
SAHA vs. CD3-0.2857749608641620.4209766083463790.53563977438196
SAHA vs. IL70.1820403696413720.4611772172245270.697480221976776
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.187101 0.293428
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
212521_s_at 1.46 No downregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00920 Ketotifen approved yes inhibitor
DB07954 3-isobutyl-1-methyl-7H-xanthine experimental unknown unknown
DB00201 Caffeine approved unknown inhibitor, competitive

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1LHQ Model - A=482-821.
1LXX Model - A=488-821.
3ECM X-ray 1.9Å A=482-819.
3ECN X-ray 2.1Å A/B=482-819.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat activates 11156964
HIV-1 virus replication enhanced by expression of human gene 18976975

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00230 Purine metabolism - Homo sapiens (human)
hsa05032 Morphine addiction - Homo sapiens (human)