Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002119
UniProt IDQ13370
Primary gene name(s)PDE3B
Synonym gene name(s)unknown
Protein namecGMP-inhibited 3',5'-cyclic phosphodiesterase B
Protein functionCyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. May play a role in fat metabolism. Regulates cAMP binding of RAPGEF3. Through simultaneous binding to RAPGEF3 and PIK3R6 assembles a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis. {ECO:0000269|PubMed:15147193, ECO:0000269|PubMed:21393242}.
Subcellular locationMembrane {ECO:0000305};
Multi-pass membrane protein {ECO:0000305}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q13370
Gene Ontology
(Biological Process)
Complete annatation
angiogenesis [GO:0001525];
cAMP catabolic process [GO:0006198];
cellular response to insulin stimulus [GO:0032869];
negative regulation of angiogenesis [GO:0016525];
negative regulation of cAMP-mediated signaling [GO:0043951];
negative regulation of cell adhesion [GO:0007162];
negative regulation of cell adhesion mediated by integrin [GO:0033629];
negative regulation of lipid catabolic process [GO:0050995];
signal transduction [GO:0007165]
Gene Ontology
(Molecular Function)
Complete annatation
3',5'-cyclic-AMP phosphodiesterase activity [GO:0004115];
3',5'-cyclic-GMP phosphodiesterase activity [GO:0047555];
3',5'-cyclic-nucleotide phosphodiesterase activity [GO:0004114];
cGMP-inhibited cyclic-nucleotide phosphodiesterase activity [GO:0004119];
metal ion binding [GO:0046872];
protein kinase B binding [GO:0043422]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
endoplasmic reticulum [GO:0005783];
Golgi apparatus [GO:0005794];
guanyl-nucleotide exchange factor complex [GO:0032045];
integral component of membrane [GO:0016021];
membrane [GO:0016020]
Protein-protein interaction111166
Phylogenetic treeQ13370
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.208689152505630.0002669189166494280.00114419849594431
AZA vs. DISU0.06183035995798130.8064218130288060.983438610789082
AZA vs. IL7-0.1842935209068680.3366161732715710.999311006273513
AZA vs. SAHA-0.4204779531792450.08598729569689670.366564619721442
DISU vs. CD31.257253389336850.000618914289891470.00273382589651517
DISU vs. IL7-0.2555144915386660.3096148979215170.693116214161995
DISU vs. SAHA-0.4809121597917010.09989720677091130.404339262293689
DMSO vs. AZA-0.07327129627575110.6603439032009661
DMSO vs. CD31.126016977983430.0004962340623235750.00185273014842742
DMSO vs. DISU-0.1364134156914830.575311084782090.931469777531738
DMSO vs. IL7-0.1041344828460650.5616361560795290.901842672763832
DMSO vs. SAHA-0.354515465873040.134340332122270.439212575170673
HIV vs. Mock in Activation0.3845319182521360.5371166936459990.999983755607037
HIV vs. Mock in Latency0.1850100477652510.2602677407578030.999834320637052
IL7 vs. CD31.031316417831120.001456466645644250.00586673483425863
SAHA vs. CD30.7635298876152010.03169213627901590.0679181589483461
SAHA vs. IL7-0.2400635444245760.3238082090205320.573705329428243
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
0.3395 0.04557

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
1.2 0.346941876 -1.4 0.075246831 -1.9 0.043753738
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.614676 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.938 1.615 1.281 1.204 1.511
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB01640 (5r)-6-(4-{[2-(3-Iodobenzyl)-3-Oxocyclohex-1-En-1-Yl]Amino}Phenyl)-5-Methyl-4,5-Dihydropyridazin-3(2h)-One experimental unknown unknown
DB01970 Hg9a-9, Nonanoyl-N-Hydroxyethylglucamide experimental unknown unknown
DB07954 3-isobutyl-1-methyl-7H-xanthine experimental unknown unknown
DB00201 Caffeine approved unknown inhibitor, competitive

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1SO2 X-ray 2.4Å A/B/C/D=654-1073.
1SOJ X-ray 2.9Å A/B/C/D/E/F/G/H/I/J/K/L=654-1073.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat activates 11156964

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00230 Purine metabolism - Homo sapiens (human)
hsa04022 cGMP-PKG signaling pathway - Homo sapiens (human)
hsa04024 cAMP signaling pathway - Homo sapiens (human)
hsa04910 Insulin signaling pathway - Homo sapiens (human)
hsa04914 Progesterone-mediated oocyte maturation - Homo sapiens (human)
hsa04922 Glucagon signaling pathway - Homo sapiens (human)
hsa04923 Regulation of lipolysis in adipocytes - Homo sapiens (human)
hsa04924 Renin secretion - Homo sapiens (human)
hsa05032 Morphine addiction - Homo sapiens (human)