Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002109
UniProt IDQ6UW60
Primary gene name(s)PCSK4
Synonym gene name(s)PC4
Protein nameProprotein convertase subtilisin/kexin type 4
Protein functionProprotein convertase involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues, By similarity. In males, important for ADAM2 processing as well as other acrosomal proteins with roles in fertilization and critical for normal fertilization events such as sperm capacitation, acrosome reaction and binding of sperm to zona pellucida, By similarity. Plays also a role in female fertility, involved in the regulation of trophoblast migration and placental development, may be through the proteolytical processing and activation of proteins such as IGF2, PubMed:16040806. May also participate in folliculogenesis in the ovaries, By similarity. {ECO:0000250|UniProtKB:P29121, ECO:0000269|PubMed:16040806}.
Subcellular locationMembrane {ECO:0000255};
Single-pass membrane protein {ECO:0000255}. Cytoplasmic vesicle, secretory vesicle, acrosome membrane {ECO:0000250|UniProtKB:P29121}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q6UW60
Gene Ontology
(Biological Process)
Complete annatation
acrosome reaction [GO:0007340];
binding of sperm to zona pellucida [GO:0007339];
fertilization [GO:0009566];
protein processing [GO:0016485];
reproductive process [GO:0022414];
sperm capacitation [GO:0048240]
Gene Ontology
(Molecular Function)
Complete annatation
serine-type endopeptidase activity [GO:0004252]
Gene Ontology
(Cellular Component)
Complete annatation
acrosomal membrane [GO:0002080];
extracellular space [GO:0005615];
integral component of membrane [GO:0016021]
Protein-protein interaction120136
Phylogenetic treeQ6UW60
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-3.27038494319441.23477542857131e-071.1660736378087e-06
AZA vs. DISU-0.0642550690222090.8239558494390480.98478550430575
AZA vs. IL7-0.5785582137464010.2234090055382060.989265982093417
AZA vs. SAHA1.246906481098220.0004654488423099230.0122660416940206
DISU vs. CD33.163394091332244.22790940035611e-085.66432693158143e-07
DISU vs. IL7-0.5268838228413320.2372776640906660.622237862215922
DISU vs. SAHA1.310998510972773.45485359352082e-050.00170715077075254
DMSO vs. AZA-0.08851064622887470.7695506285081911
DMSO vs. CD33.171542011614741.55868193130182e-071.32067172638144e-06
DMSO vs. DISU-0.02784244508971220.9205120257667980.988899823982894
DMSO vs. IL7-0.4812722453864070.2989805694458170.784531259250773
DMSO vs. SAHA1.331172283116969.23262400941427e-050.00333680758863027
HIV vs. Mock in Activationunknownunknownunknown
HIV vs. Mock in Latency0.04790148907178040.8111000473597010.999834320637052
IL7 vs. CD3unknownunknownunknown
SAHA vs. CD34.503481383209582.38012942688215e-115.71155262151497e-10
SAHA vs. IL71.822390089075530.0003462435272536530.00518814605350057
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -1.0396 0.0317981
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found