Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002087
UniProt IDQ58A45
Primary gene name(s)PAN3
Synonym gene name(s)unknown
Protein namePAB-dependent poly(A-specific ribonuclease subunit PAN3
Protein functionRegulatory subunit of the poly(A-nuclease, PAN deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A tails of RNA when the poly(A stretch is bound by poly(A-binding protein, PABP, which is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. PAN3 acts as a positive regulator for PAN activity, recruiting the catalytic subunit PAN2 to mRNA via its interaction with PABP and to miRNA targets via its interaction with GW182 family proteins. {ECO:0000255|HAMAP-Rule:MF_03181, ECO:0000269|PubMed:14583602, ECO:0000269|PubMed:23932717}.
Subcellular locationCytoplasm, P-body {ECO:0000255|HAMAP-Rule:MF_03181, ECO:0000269|PubMed:14583602, ECO:0000269|PubMed:18625844}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q58A45
Gene Ontology
(Biological Process)
Complete annatation
deadenylation-dependent decapping of nuclear-transcribed mRNA [GO:0000290];
mRNA processing [GO:0006397];
nuclear-transcribed mRNA poly(A tail shortening [GO:0000289];
positive regulation of cytoplasmic mRNA processing body assembly [GO:0010606];
protein targeting [GO:0006605];
RNA phosphodiester bond hydrolysis, exonucleolytic [GO:0090503]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
metal ion binding [GO:0046872];
poly(A RNA binding [GO:0044822];
protein kinase activity [GO:0004672]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasmic mRNA processing body [GO:0000932];
cytosol [GO:0005829];
PAN complex [GO:0031251]
Protein-protein interaction129129
Phylogenetic treeQ58A45
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.288932849484610.3779102355826510.499593287492476
AZA vs. DISU0.306642470295990.2271681262931120.814317774683638
AZA vs. IL7-0.1112909555427020.5635205574532770.999311006273513
AZA vs. SAHA-0.4257273517848270.0839670979313240.361634295490508
DISU vs. CD30.5821964662524580.1109623733713440.202303678785635
DISU vs. IL7-0.4265740085824880.09149113111229590.4011718445369
DISU vs. SAHA-0.7315311686882630.01257010457678340.120129388761384
DMSO vs. AZA-0.03820650301432640.8200801106663851
DMSO vs. CD30.2398113643571160.4535502702500120.565155899199043
DMSO vs. DISU-0.3464932466110840.1571069468783060.66134125403801
DMSO vs. IL7-0.06591086746920230.7145495276514060.942196722996068
DMSO vs. SAHA-0.3948847867531980.09688573429978240.366189710666626
HIV vs. Mock in Activation-0.03233421677381160.9586382691427590.999983755607037
HIV vs. Mock in Latency0.01593810176384270.9231620592356380.999834320637052
IL7 vs. CD30.1860155532708250.5630015012192080.684309461162578
SAHA vs. CD3-0.1622009956929820.6493965873952060.738987130714019
SAHA vs. IL7-0.318874760373980.193358843174490.424325404281884
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 1.00371 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.987 1.016 0.926 0.884 0.932
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03018 RNA degradation - Homo sapiens (human)