Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002080
UniProt IDP43034
Primary gene name(s)PAFAH1B1
Synonym gene name(s)LIS1, MDCR, MDS, PAFAHA
Protein namePlatelet-activating factor acetylhydrolase IB subunit alpha
Protein functionRequired for proper activation of Rho GTPases and actin polymerization at the leading edge of locomoting cerebellar neurons and postmigratory hippocampal neurons in response to calcium influx triggered via NMDA receptors. Non-catalytic subunit of an acetylhydrolase complex which inactivates platelet-activating factor, PAF by removing the acetyl group at the SN-2 position, By similarity. Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. Required during brain development for the proliferation of neuronal precursors and the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Neuronal migration involves a process called nucleokinesis, whereby migrating cells extend an anterior process into which the nucleus subsequently translocates. During nucleokinesis dynein at the nuclear surface may translocate the nucleus towards the centrosome by exerting force on centrosomal microtubules. May also play a role in other forms of cell locomotion including the migration of fibroblasts during wound healing. Required for dynein recruitment to microtubule plus ends and BICD2-bound cargos, PubMed:22956769. {ECO:0000250|UniProtKB:P63005, ECO:0000269|PubMed:15173193, ECO:0000269|PubMed:22956769}.
Subcellular locationCytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle {ECO:0000255|HAMAP-Rule:MF_03141}. Nucleus membrane {ECO:0000255|HAMAP-Rule:MF_03141}. Note=Redistributes to axons during neuronal development. Also localizes to the microtubules of the manchette in elongating spermatids and to the meiotic spindle in spermatocytes, By similarity. Localizes to the plus end of microtubules and to the centrosome. May localize to the nuclear membrane. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P43034
Gene Ontology
(Biological Process)
Complete annatation
acrosome assembly [GO:0001675];
actin cytoskeleton organization [GO:0030036];
adult locomotory behavior [GO:0008344];
ameboidal-type cell migration [GO:0001667];
auditory receptor cell development [GO:0060117];
brain morphogenesis [GO:0048854];
cerebral cortex development [GO:0021987];
cerebral cortex neuron differentiation [GO:0021895];
chemical synaptic transmission [GO:0007268];
cochlea development [GO:0090102];
corpus callosum morphogenesis [GO:0021540];
cortical microtubule organization [GO:0043622];
establishment of centrosome localization [GO:0051660];
establishment of mitotic spindle orientation [GO:0000132];
establishment of planar polarity of embryonic epithelium [GO:0042249];
G2/M transition of mitotic cell cycle [GO:0000086];
germ cell development [GO:0007281];
hippocampus development [GO:0021766];
layer formation in cerebral cortex [GO:0021819];
learning or memory [GO:0007611];
lipid catabolic process [GO:0016042];
maintenance of centrosome location [GO:0051661];
microtubule-based process [GO:0007017];
microtubule cytoskeleton organization [GO:0000226];
microtubule cytoskeleton organization involved in establishment of planar polarity [GO:0090176];
microtubule organizing center organization [GO:0031023];
mitotic nuclear division [GO:0007067];
negative regulation of JNK cascade [GO:0046329];
negative regulation of neuron projection development [GO:0010977];
neuroblast proliferation [GO:0007405];
neuromuscular process controlling balance [GO:0050885];
neuron migration [GO:0001764];
nuclear envelope disassembly [GO:0051081];
nuclear migration [GO:0007097];
osteoclast development [GO:0036035];
platelet activating factor metabolic process [GO:0046469];
positive regulation of axon extension [GO:0045773];
positive regulation of cytokine-mediated signaling pathway [GO:0001961];
positive regulation of dendritic spine morphogenesis [GO:0061003];
positive regulation of embryonic development [GO:0040019];
positive regulation of mitotic cell cycle [GO:0045931];
protein secretion [GO:0009306];
regulation of GTPase activity [GO:0043087];
regulation of microtubule cytoskeleton organization [GO:0070507];
regulation of microtubule motor activity [GO:2000574];
retrograde axonal transport [GO:0008090];
sister chromatid cohesion [GO:0007062];
stem cell division [GO:0017145];
transmission of nerve impulse [GO:0019226];
vesicle transport along microtubule [GO:0047496]
Gene Ontology
(Molecular Function)
Complete annatation
dynactin binding [GO:0034452];
dynein binding [GO:0045502];
heparin binding [GO:0008201];
microtubule binding [GO:0008017];
phospholipase A2 activity [GO:0004623];
phospholipase binding [GO:0043274];
phosphoprotein binding [GO:0051219];
protein homodimerization activity [GO:0042803]
Gene Ontology
(Cellular Component)
Complete annatation
astral microtubule [GO:0000235];
axon cytoplasm [GO:1904115];
cell cortex [GO:0005938];
cell leading edge [GO:0031252];
centrosome [GO:0005813];
cytosol [GO:0005829];
extracellular exosome [GO:0070062];
growth cone [GO:0030426];
kinesin complex [GO:0005871];
kinetochore [GO:0000776];
microtubule associated complex [GO:0005875];
motile primary cilium [GO:0031512];
neuronal cell body [GO:0043025];
nuclear envelope [GO:0005635];
nuclear membrane [GO:0031965];
perinuclear region of cytoplasm [GO:0048471]
Protein-protein interaction111085
Phylogenetic treeP43034
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.05341490349767710.8703512479495490.914514302439844
AZA vs. DISU0.0003614314105308870.9988577749760870.999781898394592
AZA vs. IL70.08414292188915810.660817029780820.999311006273513
AZA vs. SAHA-0.1579848747387290.5164583854333990.831295919695773
DISU vs. CD30.04161451181514590.908453850491160.940201396656625
DISU vs. IL70.07472953192575270.7662654167225340.945255705517515
DISU vs. SAHA-0.1572651301176510.5888092633553240.868538144966454
DMSO vs. AZA-0.03418516572224770.8377978861152951
DMSO vs. CD30.008921688322068260.9777189201491980.984515442286456
DMSO vs. DISU-0.03613203371324130.8820367090083630.987443803738299
DMSO vs. IL70.1254758168013210.4841327157274640.881947455392084
DMSO vs. SAHA-0.1310539456153440.577539240240320.853735167863326
HIV vs. Mock in Activation0.009039992567060960.9884180339085610.999983755607037
HIV vs. Mock in Latency-0.001525094191732670.9926036438443150.999834320637052
IL7 vs. CD30.145406703911570.6504002592965910.758582300649097
SAHA vs. CD3-0.1295300673071480.7137051750854040.79184314897572
SAHA vs. IL7-0.2458626615060190.3119644567926430.560834900097858
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.381996 0.00420274
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.027 0.94 1.025 1.095 0.927
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
-1.53 0.0006 -0.3 0.1413 -0.07 0.9947 Cell growth and proliferation at 4 hpi
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat interacts with 15698475

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00565 Ether lipid metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)