Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002072
UniProt IDQ9UQ80
Primary gene name(s)PA2G4
Synonym gene name(s)EBP1
Protein nameProliferation-associated protein 2G4
Protein functionMay play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor, AR and is regulated by the ERBB3 ligand neuregulin-1/heregulin, HRG. Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase, HAT activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs, internal ribosomal entry site, By similarity. Regulates cell proliferation, differentiation, and survival. Isoform 1 suppresses apoptosis whereas isoform 2 promotes cell differentiation, By similarity. {ECO:0000250|UniProtKB:P50580, ECO:0000250|UniProtKB:Q6AYD3, ECO:0000269|PubMed:11268000, ECO:0000269|PubMed:12682367, ECO:0000269|PubMed:15064750, ECO:0000269|PubMed:15583694, ECO:0000269|PubMed:16832058}.
Subcellular locationIsoform 1: Cytoplasm {ECO:0000269|PubMed:16832058, ECO:0000269|PubMed:19037095}. Nucleus, nucleolus {ECO:0000269|PubMed:16832058, ECO:0000269|PubMed:19037095}. Note=Tranlocates to the nucleus upon treatment with HRG. Phosphorylation at Ser-361 by PKC/PRKCD regulates its nucleolar localization. {ECO:0000269|PubMed:16832058}.;
SUBCELLULAR LOCATION: Isoform 2: Cytoplasm {ECO:0000269|PubMed:16832058, ECO:0000269|PubMed:19037095}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9UQ80
Gene Ontology
(Biological Process)
Complete annatation
cell cycle arrest [GO:0007050];
cell proliferation [GO:0008283];
negative regulation of apoptotic process [GO:0043066];
negative regulation of transcription, DNA-templated [GO:0045892];
positive regulation of cell differentiation [GO:0045597];
regulation of translation [GO:0006417];
rRNA processing [GO:0006364];
transcription, DNA-templated [GO:0006351]
Gene Ontology
(Molecular Function)
Complete annatation
DNA binding [GO:0003677];
poly(A RNA binding [GO:0044822];
transcription factor activity, sequence-specific DNA binding [GO:0003700];
ubiquitin protein ligase binding [GO:0031625]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
extracellular exosome [GO:0070062];
intracellular ribonucleoprotein complex [GO:0030529];
membrane [GO:0016020];
nucleolus [GO:0005730];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction111075
Phylogenetic treeQ9UQ80
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.676613862633885.87704744647866e-074.81668619430812e-06
AZA vs. DISU-0.1491164494599740.5551019827656180.948600758214366
AZA vs. IL70.4383305837648540.02252372480590310.478342905010373
AZA vs. SAHA-0.07396876131132340.7616313179711560.936140776834411
DISU vs. CD3-1.83733609376988.92137127284087e-078.70989317142508e-06
DISU vs. IL70.5781971765360740.02198932246076930.187918954703003
DISU vs. SAHA0.07668624351907510.7927906763714830.944741700292134
DMSO vs. AZA-0.01443673241904220.9311501001273981
DMSO vs. CD3-1.701166405512.13953827987723e-071.75365311133572e-06
DMSO vs. DISU0.1331398182353080.5849522256616040.933630558826625
DMSO vs. IL70.459972012864970.01047973217153310.212913696072589
DMSO vs. SAHA-0.06605834954566160.7791693639720880.937553811131604
HIV vs. Mock in Activation-0.0931719308045430.8808091474664620.999983755607037
HIV vs. Mock in Latency-0.004224508229252050.9795885870383020.999834320637052
IL7 vs. CD3-1.231013043116330.0001548026510242990.000853140899045435
SAHA vs. CD3-1.774508418718731.02801606727265e-068.31572846688077e-06
SAHA vs. IL7-0.5147000315746160.03486971303343870.144071064314803
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change -0.967110976
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.459795 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.011 0.953 0.864 0.823 0.864
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2Q8K X-ray 1.6Å A=2-394.
3J2I EM 11.9Å A=1-394.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
retropepsin cleaves 22944692
Vpr downregulates 23828502

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found