Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002070
UniProt IDQ99571
Primary gene name(s)P2RX4
Synonym gene name(s)unknown
Protein nameP2X purinoceptor 4
Protein functionReceptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin. {ECO:0000269|PubMed:10515189}.
Subcellular locationMembrane {ECO:0000269|PubMed:10515189};
Multi-pass membrane protein {ECO:0000269|PubMed:10515189}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q99571
Gene Ontology
(Biological Process)
Complete annatation
apoptotic signaling pathway [GO:0097190];
blood coagulation [GO:0007596];
cellular response to ATP [GO:0071318];
endothelial cell activation [GO:0042118];
ion transmembrane transport [GO:0034220];
membrane depolarization [GO:0051899];
negative regulation of cardiac muscle hypertrophy [GO:0010614];
neuronal action potential [GO:0019228];
positive regulation of calcium ion transport [GO:0051928];
positive regulation of calcium ion transport into cytosol [GO:0010524];
positive regulation of calcium-mediated signaling [GO:0050850];
positive regulation of nitric oxide biosynthetic process [GO:0045429];
positive regulation of prostaglandin secretion [GO:0032308];
protein homooligomerization [GO:0051260];
purinergic nucleotide receptor signaling pathway [GO:0035590];
regulation of blood pressure [GO:0008217];
regulation of cardiac muscle contraction [GO:0055117];
regulation of sodium ion transport [GO:0002028];
relaxation of cardiac muscle [GO:0055119];
response to ATP [GO:0033198];
response to fluid shear stress [GO:0034405];
sensory perception of pain [GO:0019233];
signal transduction [GO:0007165];
tissue homeostasis [GO:0001894];
transport [GO:0006810]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
cadherin binding [GO:0045296];
copper ion binding [GO:0005507];
drug binding [GO:0008144];
extracellular ATP-gated cation channel activity [GO:0004931];
protein homodimerization activity [GO:0042803];
purinergic nucleotide receptor activity [GO:0001614];
receptor binding [GO:0005102];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
cell junction [GO:0030054];
dendritic spine [GO:0043197];
extracellular exosome [GO:0070062];
integral component of nuclear inner membrane [GO:0005639];
integral component of plasma membrane [GO:0005887];
lysosomal membrane [GO:0005765];
membrane [GO:0016020];
neuronal cell body [GO:0043025];
perinuclear region of cytoplasm [GO:0048471];
plasma membrane [GO:0005886];
postsynaptic density [GO:0014069];
terminal bouton [GO:0043195]
Protein-protein interaction111064
Phylogenetic treeQ99571
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.766804371781171.87011320873509e-071.69389100252736e-06
AZA vs. DISU-0.4503088194205460.07625776185212030.590222266291186
AZA vs. IL7-0.1674210648148630.3859660421307440.999311006273513
AZA vs. SAHA-1.105441274936750.0001273745500625090.00454986722190109
DISU vs. CD31.304175052808780.0004385229166676830.00204021433513847
DISU vs. IL70.2738200740781480.2785608495402840.665801817787118
DISU vs. SAHA-0.6536765791496960.02904766521434840.202956588987225
DMSO vs. AZA0.06134415431577770.7156470572780711
DMSO vs. CD31.811602707839664.58939874992126e-084.28107072244977e-07
DMSO vs. DISU0.5085677460409810.03799511710271430.393259900232077
DMSO vs. IL7-0.2208958984169750.2213959331472070.729446701738132
DMSO vs. SAHA-1.171743959553238.93894928469807e-050.00326195895083982
HIV vs. Mock in Activation-0.09566521152333760.8783248597882830.999983755607037
HIV vs. Mock in Latency-0.1156575951815830.4852753025988160.999834320637052
IL7 vs. CD31.606935097369091.18839166429741e-061.14057577665756e-05
SAHA vs. CD30.6368179843957790.07483816467021430.138042979385573
SAHA vs. IL7-0.9419656434105360.0009047939015699450.0107902399305897
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.29579 0.0500226
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB09119 Eslicarbazepine acetate approved yes antagonist

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04020 Calcium signaling pathway - Homo sapiens (human)
hsa04080 Neuroactive ligand-receptor interaction - Homo sapiens (human)