Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002065
UniProt IDQ6GQQ9
Primary gene name(s)OTUD7B
Synonym gene name(s)ZA20D1
Protein nameOTU domain-containing protein 7B
Protein functionNegative regulator of the non-canonical NF-kappa-B pathway that acts by mediating deubiquitination of TRAF3, an inhibitor of the NF-kappa-B pathway, thereby acting as a negative regulator of B-cell responses. In response to non-canonical NF-kappa-B stimuli, deubiquitinates 'Lys-48'-linked polyubiquitin chains of TRAF3, preventing TRAF3 proteolysis and over-activation of non-canonical NF-kappa-B. Negatively regulates mucosal immunity against infections. Mediates deubiquitination of EGFR. Has deubiquitinating activity toward 'Lys-11', 'Lys-48' or 'Lys-63'-linked polyubiquitin chains. In vitro, has preference for 'Lys-11'-linked polyubiquitin chains; however such data are unsure in vivo. Hydrolyzes both linear and branched forms of polyubiquitin. {ECO:0000269|PubMed:11463333, ECO:0000269|PubMed:12682062, ECO:0000269|PubMed:18178551, ECO:0000269|PubMed:20622874, ECO:0000269|PubMed:22179831, ECO:0000269|PubMed:23827681}.
Subcellular locationCytoplasm. Nucleus. Note=Shuttles be cytoplasm and the nucleus in a XPO1/CRM1-dependent manner.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q6GQQ9
Gene Ontology
(Biological Process)
Complete annatation
immune response [GO:0006955];
mucosal immune response [GO:0002385];
negative regulation of I-kappaB kinase/NF-kappaB signaling [GO:0043124];
negative regulation of interleukin-8 production [GO:0032717];
negative regulation of protein localization to nucleus [GO:1900181];
negative regulation of transcription from RNA polymerase II promoter [GO:0000122];
protein deubiquitination involved in ubiquitin-dependent protein catabolic process [GO:0071947];
protein K11-linked deubiquitination [GO:0035871];
protein K48-linked deubiquitination [GO:0071108];
protein K63-linked deubiquitination [GO:0070536]
Gene Ontology
(Molecular Function)
Complete annatation
cysteine-type peptidase activity [GO:0008234];
DNA binding [GO:0003677];
K63-linked polyubiquitin binding [GO:0070530];
Lys48-specific deubiquitinase activity [GO:1990380];
thiol-dependent ubiquitin-specific protease activity [GO:0004843];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
nucleus [GO:0005634]
Protein-protein interaction121281
Phylogenetic treeQ6GQQ9
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.658810189999321.01610428304699e-067.85773409650296e-06
AZA vs. DISU0.2456776768493880.3372240544632870.880262543573058
AZA vs. IL7-0.0662612484551390.736383218258870.999311006273513
AZA vs. SAHA0.5099981783951620.03958000521186330.235078407629483
DISU vs. CD3-1.424888606680850.0001471122771063980.000794258762572294
DISU vs. IL7-0.3214577441026740.2069108599544480.585871753158022
DISU vs. SAHA0.2656542149690150.3677512150328420.739865038264888
DMSO vs. AZA-0.1000800499999050.5629267474233261
DMSO vs. CD3-1.767664533179071.1255211318506e-079.83427696491882e-07
DMSO vs. DISU-0.347068815770240.1598806609927680.665396730505664
DMSO vs. IL70.04064508701079860.8254890684744010.963051812112721
DMSO vs. SAHA0.6025390781244210.01205966567720380.101714680261655
HIV vs. Mock in Activation0.1917298443423080.7580280576260860.999983755607037
HIV vs. Mock in Latency0.1359766977874970.4220275547935340.999834320637052
IL7 vs. CD3-1.718500288565572.90117621148944e-073.19659568975003e-06
SAHA vs. CD3-1.174023786849640.001077861456120880.00381568525810233
SAHA vs. IL70.5729447477552310.0208878499693380.103393619810645
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.13757 0.401232
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.862 0.98 0.943 0.898 0.918
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
5LRU X-ray 2.2Å A=129-438.
5LRV X-ray 2.8Å A=129-438.
5LRW X-ray 2.0Å A/C=129-266# A/C=292-438.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found