Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002058
UniProt IDQ9H4L5
Primary gene name(s)OSBPL3
Synonym gene name(s)KIAA0704, ORP3, OSBP3
Protein nameOxysterol-binding protein-related protein 3
Protein functionPhosphoinositide-binding protein which associates with both cell and endoplasmic reticulum, ER membranes, PubMed:16143324. Can bind to the ER membrane protein VAPA and recruit VAPA to plasma membrane sites, thus linking these intracellular compartments, PubMed:25447204. The ORP3-VAPA complex stimulates RRAS signaling which in turn attenuates integrin beta-1, ITGB1 activation at the cell surface, PubMed:18270267, PubMed:25447204. With VAPA, may regulate ER morphology, PubMed:16143324. Has a role in regulation of the actin cytoskeleton, cell polarity and cell adhesion, PubMed:18270267. Binds to phosphoinositides with preference for PI(3,4P2 and PI(3,4,5P3, PubMed:16143324. Also binds 25-hydroxycholesterol and cholesterol, PubMed:17428193. {ECO:0000269|PubMed:16143324, ECO:0000269|PubMed:17428193, ECO:0000269|PubMed:18270267, ECO:0000269|PubMed:25447204}.
Subcellular locationEndoplasmic reticulum membrane {ECO:0000269|PubMed:16143324};
Peripheral membrane protein {ECO:0000269|PubMed:16143324}. Cytoplasm, cytosol {ECO:0000269|PubMed:16143324}. Cell membrane {ECO:0000269|PubMed:14593528, ECO:0000269|PubMed:16143324, ECO:0000269|PubMed:18270267, ECO:0000269|PubMed:25447204};
Peripheral membrane protein {ECO:0000269|PubMed:14593528, ECO:0000269|PubMed:16143324, ECO:0000269|PubMed:18270267, ECO:0000269|PubMed:25447204}. Cell projection, filopodium tip {ECO:0000269|PubMed:18270267}. Nucleus membrane {ECO:0000269|PubMed:16143324};
Peripheral membrane protein {ECO:0000305}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9H4L5
Gene Ontology
(Biological Process)
Complete annatation
lipid transport [GO:0006869]
Gene Ontology
(Molecular Function)
Complete annatation
cholesterol binding [GO:0015485]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
endoplasmic reticulum membrane [GO:0005789];
filopodium tip [GO:0032433];
membrane [GO:0016020];
nuclear membrane [GO:0031965];
perinuclear endoplasmic reticulum [GO:0097038];
plasma membrane [GO:0005886]
Protein-protein interaction117497
Phylogenetic treeQ9H4L5
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.009853612612440.002234207203084650.00728473430726578
AZA vs. DISU0.2064887070687660.4143023791524340.910230653368199
AZA vs. IL70.1849492131542120.335694404752230.999311006273513
AZA vs. SAHA-0.1849821325039650.4480708870282120.790855173834205
DISU vs. CD3-0.8170166984382310.02486121444291540.0614458020187748
DISU vs. IL7-0.03015867732649290.9046114276835260.982189124739072
DISU vs. SAHA-0.3897801989879380.1812927577337970.5470274332007
DMSO vs. AZA0.01452499520501210.9308521431700071
DMSO vs. CD3-1.00802063886610.001772322938756730.00561279063955648
DMSO vs. DISU-0.1939649104589560.4263708081622760.880629497839006
DMSO vs. IL70.1777543666921540.3224430718635120.799721223986312
DMSO vs. SAHA-0.2058029081499520.3824258723854760.729949905970682
HIV vs. Mock in Activation-0.05201027325984610.9335849925121850.999983755607037
HIV vs. Mock in Latency-0.06019157119408350.7153529509357190.999834320637052
IL7 vs. CD3-0.8168700504908380.01140986853898670.0332073659950081
SAHA vs. CD3-1.219434050157710.0006551372168128730.00248229768718292
SAHA vs. IL7-0.3734996278557130.1251924306011880.328542948969762
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
0.4371 0.03139

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.416751 0.0017721
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.994 1.075 1.181 1.195 1.326
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Pol interacts with 22190034
retropepsin interacts with 22190034
HIV-1 virus replication enhanced by expression of human gene 18854154

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found