Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002051
UniProt IDQ96CV9
Primary gene name(s)OPTN
Synonym gene name(s)FIP2, GLC1E, HIP7, HYPL, NRP
Protein nameOptineurin
Protein functionPlays an important role in the maintenance of the Golgi complex, in membrane trafficking, in exocytosis, through its interaction with myosin VI and Rab8. Links myosin VI to the Golgi complex and plays an important role in Golgi ribbon formation. Negatively regulates the induction of IFNB in response to RNA virus infection. Plays a neuroprotective role in the eye and optic nerve. Probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. May act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin, HD. Mediates the interaction of Rab8 with the probable GTPase-activating protein TBC1D17 during Rab8-mediated endocytic trafficking, such as of transferrin receptor, TFRC/TfR; regulates Rab8 recruitnment to tubules emanating from the endocytic recycling compartment. Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family; targets ubiquitin-coated bacteria, xenophagy, such as cytoplasmic Salmonella enterica, and appears to function in the same pathway as SQSTM1 and CALCOCO2/NDP52. May constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. {ECO:0000269|PubMed:11834836, ECO:0000269|PubMed:15837803, ECO:0000269|PubMed:20085643, ECO:0000269|PubMed:20174559, ECO:0000269|PubMed:21617041, ECO:0000269|PubMed:22854040}.
Subcellular locationCytoplasm, perinuclear region. Golgi apparatus. Golgi apparatus, trans-Golgi network. Cytoplasmic vesicle, autophagosome. Cytoplasmic vesicle. Recycling endosome. Note=Found in the perinuclear region and associates with the Golgi apparatus. Colocalizes with MYO6 and RAB8 at the Golgi complex and in vesicular structures close to the plasma membrane. Localizes to LC3-positive cytoplasmic vesicles upon induction of autophagy.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q96CV9
Gene Ontology
(Biological Process)
Complete annatation
cell death [GO:0008219];
defense response to Gram-negative bacterium [GO:0050829];
G2/M transition of mitotic cell cycle [GO:0000086];
Golgi organization [GO:0007030];
Golgi ribbon formation [GO:0090161];
Golgi to plasma membrane protein transport [GO:0043001];
macroautophagy [GO:0016236];
negative regulation of I-kappaB kinase/NF-kappaB signaling [GO:0043124];
negative regulation of receptor recycling [GO:0001920];
parkin-mediated mitophagy in response to mitochondrial depolarization [GO:0061734];
protein targeting to Golgi [GO:0000042];
regulation of I-kappaB kinase/NF-kappaB signaling [GO:0043122];
signal transduction [GO:0007165];
xenophagy [GO:0098792]
Gene Ontology
(Molecular Function)
Complete annatation
identical protein binding [GO:0042802];
K63-linked polyubiquitin binding [GO:0070530];
polyubiquitin binding [GO:0031593];
protein binding, bridging [GO:0030674];
protein C-terminus binding [GO:0008022];
Rab GTPase binding [GO:0017137]
Gene Ontology
(Cellular Component)
Complete annatation
autophagosome [GO:0005776];
cytoplasm [GO:0005737];
cytoplasmic, membrane-bounded vesicle [GO:0016023];
cytosol [GO:0005829];
Golgi apparatus [GO:0005794];
Golgi membrane [GO:0000139];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
perinuclear region of cytoplasm [GO:0048471];
recycling endosome [GO:0055037];
trans-Golgi network [GO:0005802]
Protein-protein interaction115436
Phylogenetic treeQ96CV9
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.537495832177364.59704979538511e-063.07258246190884e-05
AZA vs. DISU0.387788593531160.1251244725353120.6876143999261
AZA vs. IL7-0.3513808307331720.06763285420008280.741943239707313
AZA vs. SAHA0.05576627642200450.8191396860533190.95496609648629
DISU vs. CD31.913024609900553.45849098470907e-073.72646841340276e-06
DISU vs. IL7-0.7486455712165910.003079788811006010.0560722419395116
DISU vs. SAHA-0.3306902668831640.2567748989101630.634850298482705
DMSO vs. AZA-0.104550614027080.5318648588085151
DMSO vs. CD31.422905448764831.30598147475292e-057.34401303365161e-05
DMSO vs. DISU-0.4939042634963830.04307088945724810.414926764252068
DMSO vs. IL7-0.239821690202590.1820076228195250.692439484501877
DMSO vs. SAHA0.1531615348857850.5161953646751770.821931361967205
HIV vs. Mock in Activation0.09937050387837760.8731233873687560.999983755607037
HIV vs. Mock in Latency0.1910847457782780.2460054483164960.999834320637052
IL7 vs. CD31.193357799566580.000249742702804090.00128828602305477
SAHA vs. CD31.568381529378771.65304403243471e-059.72720561061739e-05
SAHA vs. IL70.4035193800778210.09869445434534060.284894715588216
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.295704 0.0259706
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.044 1.178 1.424 1.601 1.204
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2LO4 NMR - A=550-577.
2LUE NMR - B=169-185.
3VTV X-ray 1.7Å A=170-181.
3VTW X-ray 2.5Å A/B/C=170-181.
5AAZ NMR - A=548-577.
5B83 X-ray 2.6Å B/C/E/F=416-510.
5EOA X-ray 2.5Å A/B=26-103.
5EOF X-ray 2.0Å A/B=26-103.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found