Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002045
UniProt IDQ01968
Primary gene name(s)OCRL
Synonym gene name(s)INPP5F, OCRL1
Protein nameInositol polyphosphate 5-phosphatase OCRL-1
Protein functionConverts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol 4-phosphate. Also converts inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate and inositol 1,3,4,5-tetrakisphosphate to inositol 1,3,4-trisphosphate, PubMed:25869668, PubMed:7761412, PubMed:9430698. May function in lysosomal membrane trafficking by regulating the specific pool of phosphatidylinositol 4,5-bisphosphate that is associated with lysosomes. Involved in primary cilia assembly, PubMed:22228094, PubMed:22543976. {ECO:0000269|PubMed:22228094, ECO:0000269|PubMed:22543976, ECO:0000269|PubMed:25869668, ECO:0000269|PubMed:7761412, ECO:0000269|PubMed:9430698}.
Subcellular locationCytoplasmic vesicle, phagosome membrane {ECO:0000250|UniProtKB:D3ZGS3}. Early endosome membrane {ECO:0000269|PubMed:25869668}. Membrane, clathrin-coated pit {ECO:0000269|PubMed:25869668}. Cell projection, cilium, photoreceptor outer segment {ECO:0000269|PubMed:22543976}. Cell projection, cilium {ECO:0000269|PubMed:22543976}. Cytoplasmic vesicle {ECO:0000250|UniProtKB:D3ZGS3}. Endosome {ECO:0000269|PubMed:25869668}. Golgi apparatus, trans-Golgi network {ECO:0000250|UniProtKB:D3ZGS3}. Note=Also found on macropinosomes. {ECO:0000269|PubMed:25869668}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q01968
Gene Ontology
(Biological Process)
Complete annatation
cilium assembly [GO:0060271];
inositol phosphate metabolic process [GO:0043647];
in utero embryonic development [GO:0001701];
lipid metabolic process [GO:0006629];
phosphatidylinositol biosynthetic process [GO:0006661];
phosphatidylinositol dephosphorylation [GO:0046856];
regulation of GTPase activity [GO:0043087];
regulation of small GTPase mediated signal transduction [GO:0051056];
signal transduction [GO:0007165]
Gene Ontology
(Molecular Function)
Complete annatation
GTPase activator activity [GO:0005096];
inositol-1,3,4,5-tetrakisphosphate 5-phosphatase activity [GO:0052659];
inositol-1,4,5-trisphosphate 5-phosphatase activity [GO:0052658];
inositol phosphate phosphatase activity [GO:0052745];
phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity [GO:0004439];
Rac GTPase binding [GO:0048365]
Gene Ontology
(Cellular Component)
Complete annatation
clathrin-coated vesicle [GO:0030136];
coated pit [GO:0005905];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
early endosome [GO:0005769];
early endosome membrane [GO:0031901];
extracellular exosome [GO:0070062];
Golgi-associated vesicle [GO:0005798];
Golgi stack [GO:0005795];
nucleus [GO:0005634];
phagocytic vesicle membrane [GO:0030670];
photoreceptor outer segment [GO:0001750];
plasma membrane [GO:0005886];
trans-Golgi network [GO:0005802]
Protein-protein interaction111006
Phylogenetic treeQ01968
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.5077264067308180.1722114466762670.271613686737846
AZA vs. DISU-0.04702134354150250.9165436380261430.994271142470137
AZA vs. IL7-0.1360510755743780.7490563652788940.999311006273513
AZA vs. SAHA0.9861732202365490.03277433596701880.209508822349925
DISU vs. CD3-0.5719492484990090.1255227335515790.222734512720011
DISU vs. IL7-0.0982079420947290.7740717501851580.947631284232683
DISU vs. SAHA1.034580798018220.007574386882763020.0858379332543203
DMSO vs. AZA-0.0557552276512080.9007256399577791
DMSO vs. CD3-0.5764176892040340.08281258128041420.146480161675407
DMSO vs. DISU-0.01091617593419690.9762349756414410.995569325920738
DMSO vs. IL7-0.07341071582375370.8279480107516820.963051812112721
DMSO vs. SAHA1.033893619008240.007001954448117020.0707756240694645
HIV vs. Mock in Activation-0.03584035890138020.9549360625160310.999983755607037
HIV vs. Mock in Latency-0.2131043696580860.2396241031472330.999834320637052
IL7 vs. CD3-0.6381532692170020.05629682633175980.121153921087752
SAHA vs. CD30.4496488481985510.2152124886160690.319784283518453
SAHA vs. IL71.117330514484350.001925606153852620.0191847899293949
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.224686 0.297603
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2KIE NMR - A=1-119.
2QV2 X-ray 2.4Å A=564-901.
3QBT X-ray 2.0Å B/D/F/H=540-678.
3QIS X-ray 2.3Å A=536-901.
4CMN X-ray 3.1Å A=215-560.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00562 Inositol phosphate metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa04070 Phosphatidylinositol signaling system - Homo sapiens (human)