Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002038
UniProt IDP61970
Primary gene name(s)NUTF2
Synonym gene name(s)NTF2
Protein nameNuclear transport factor 2
Protein functionMediates the import of GDP-bound RAN from the cytoplasm into the nucleus which is essential for the function of RAN in cargo receptor-mediated nucleocytoplasmic transport. Thereby, plays indirectly a more general role in cargo receptor-mediated nucleocytoplasmic transport. Interacts with GDP-bound RAN in the cytosol, recruits it to the nuclear pore complex via its interaction with nucleoporins and promotes its nuclear import. {ECO:0000269|PubMed:10679025, ECO:0000269|PubMed:7744965}.
Subcellular locationCytoplasm, cytosol {ECO:0000269|PubMed:10679025, ECO:0000269|PubMed:7744965}. Nucleus outer membrane {ECO:0000250|UniProtKB:P61972}. Nucleus, nuclear pore complex {ECO:0000250|UniProtKB:P61972}. Nucleus inner membrane {ECO:0000250|UniProtKB:P61972}. Nucleus, nucleoplasm {ECO:0000269|PubMed:10679025}. Note=At steady state it is essentially nucleoplasmic, enriched in nucleoplasmic foci. {ECO:0000269|PubMed:10679025}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P61970
Gene Ontology
(Biological Process)
Complete annatation
mRNA transport [GO:0051028];
negative regulation of vascular endothelial growth factor production [GO:1904046];
positive regulation of protein import into nucleus [GO:0042307];
protein export from nucleus [GO:0006611];
protein import into nucleus [GO:0006606];
protein import into nucleus, translocation [GO:0000060];
protein localization to nuclear pore [GO:0090204]
Gene Ontology
(Molecular Function)
Complete annatation
Ran GTPase binding [GO:0008536];
transporter activity [GO:0005215]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
extracellular exosome [GO:0070062];
nuclear outer membrane [GO:0005640];
nuclear pore [GO:0005643];
nuclear pore central transport channel [GO:0044613]
Protein-protein interaction115499
Phylogenetic treeP61970
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.360738849612914.42785789077638e-050.000234657785266461
AZA vs. DISU-0.09208900922534460.7163665505087290.973233942294547
AZA vs. IL70.3577150718767530.06412263567321930.725817192830414
AZA vs. SAHA-0.1135824133288490.6424318417053880.889518845302618
DISU vs. CD3-1.46503630847177.3960585184718e-050.000435555919527929
DISU vs. IL70.4402315387065260.08153467022509770.377730532773002
DISU vs. SAHA-0.01913740985339180.9476947679591270.988354290216041
DMSO vs. AZA0.03193027073150870.8498171112868231
DMSO vs. CD3-1.340138562739753.75177178381092e-050.000187588589190546
DMSO vs. DISU0.1222229806846320.6171186540317850.940058258149852
DMSO vs. IL70.3330515473402630.06523838357052850.488496279171841
DMSO vs. SAHA-0.1509283667139080.5231561196924350.82614275569918
HIV vs. Mock in Activation-0.06799155593664520.9130328404239930.999983755607037
HIV vs. Mock in Latency-0.1116515660006410.5024003225478980.999834320637052
IL7 vs. CD3-0.9968180606075170.002095808056532870.00802390521618489
SAHA vs. CD3-1.497566621163773.30328576769734e-050.000180757140592589
SAHA vs. IL7-0.4731814604178340.0529988877548120.191767939034094
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.184088 0.499697
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.03 0.869 0.955 0.974 0.908
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1GY5 X-ray 2.3Å A/B=1-127.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
integrase inhibited by 19369352
matrix complexes with 9562972

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found